Identification of phosphopeptide ligands for the Src-homology 2 (SH2) domain of Grb2 by phage display

European Journal of Biochemistry

Volumn 246, Issue 3, 1997, Pages 633-637

  Gram, Hermann ^a   Schmitz, Rita ^a   Zuber, Jean François ^a   Baumann, Götz ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Grb2; Peptide library; Phage display; SH2 domain

Indexed keywords

LIGAND; PHOSPHOPEPTIDE; PHOSPHOTYROSINE;

ARTICLE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

TRIXIS;

EID: 0030917459     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00633.x     Document Type: Article

References (15)

1. Baumann, G., Maier, D., Freuler, F., Tschopp, C., Baudisch, K. & Wienands, J. (1994) In vitro characterization of major ligands for Src homology 2 domains derived from protein tyrosine kinases, from the adaptor protein Shc and from GTPase-activating protein in Ramos B cells, Eur. J. Immunol. 24, 1799-1807.
2. Borrello, M. G., Pelicci, G., Arighi, E., De Filippis, L., Greco, A., Bongarzone, I., Rizzetti, M., Pelicci, P. G. & Pierotti, M. A. (1994) The oncogenic versions of the Ret and Trk tyrosine kinases bind Shc and Grb2 adaptor proteins, Oncogene 9, 1661-1668.
3. Eck, M. J., Shoelson, S. E. & Harrison, S. C. (1993) Recognition of a high-affinity phosphotyrosyl peptide by the Src homology-2 domain of p561ck, Nature 362, 87-91.
4. Lowenstein, E. J., Daly, R. J., Batzer, A. G., Li, W., Margolis, B., Lammers, R., Ullrich, A., Skolnik, E. Y., Bar Sagi, D. & Schlessinger, J. (1992) The SH2 and SH3 domain-containing protein Grb2 links receptor tyrosine kinases to ras signaling, Cell 70, 431-442.
5. Müller, K., Gombert, F. O., Manning, U., Grossmüller, F., Graff, P., Zaegel, H., Zuber, J., Freuler, F., Tschopp, C. & Baumann, G. (1996) Rapid identification of phosphopeptide ligands for SH2 domains, J. Biol. Chem. 271, 16 500-16 505.
6. Overduin, M., Rios, C. B., Mayer, B. J., Baltimore, D. & Cowburn, D. (1992) Three-dimensional solution structure of the src homology 2 domain of c-abl, Cell 70, 697-704.
7. Rozakis-Adcock, M., McGlade, J., Mbamalu, G., Pelicci, G., Daly, R., Li, W., Batzer, A., Thomas, S., Brugge, J., Pelicci, P. G., Schlessinger, J. & Pawson, T. (1992) Association of the Shc and Grb2/ Sem5 SH2-containing proteins is implicated in activation of the Ras pathway by tyrosine kinases, Nature 360, 689-692.
8. Russell, R. B., Breed, J. & Barton, G. J. (1992) Conservation analysis and structure prediction of the SH2 family of phosphotyrosine binding domains, FEBS Lett. 304, 15-20.
9. Salcini, A. E., McGlade, J., Pelicci, G., Nicoletti, I., Pawson, T. & Pelicci, P. (1994) Formation of Shc-Grb2 complexes is necessary to induce neoplastic transformation by overexpression of Shc proteins, Oncogene 9, 2827-2836.
10. Schmitz, R., Baumann, G. & Gram, H. (1996) Catalytic specificity of phosphotyrosine kinases Blk, Lyn, c-Src and Syk as assessed by phage display, J. Mol. Biol. 261, 664-677.
11. Sierke, S. L., Longo, G. M. & Koland, J. G. (1993) Structural basis of interactions between epidermal growth factor receptor and SH2 domain proteins, Biochem. Biophys. Res. Commun. 191, 45-54.
12. Smith, G. P. (1991) Surface presentation of protein epitopes using bacteriophage expression systems, Curr. Opin. Biotechnol. 2, 668-673.
13. Songyang, Z., Shoelson, S. E., McGlade, J., Olivier, P., Pawson, T., Bustelo, X. R., Barbacid, M., Sabe, H., Hanafusa, H., Yi, T., Ren, R., Baltimore, D., Ratnofsky, S., Feldman, R. A., Cantley, L. C. (1994) Specific motifs recognized by the SH2 domains of Csk, 3BP2, fps/ fes, GRB-2, HCP, SHC, Syk, and Vav, Mol. Cell. Biol. 14, 2777-2785.
14. Songyang, Z., Carraway, K. L. I., Eck, M. E., Harrison, S. C., Feldman, R. A., Mohammadi, M., Schlessinger, J., Hubbard, S. R., Smith, D. P., Eng, C., Lorenzo, M. J., Ponder, B. A. J., Mayer, B. & Cantley, L. C. (1995) Catalytic specificity ot protein-tyrosine kinases is critical for selective signalling, Nature 373, 536-539.
15. Wells, J. A. & Lowman, H. B. (1992) Rapid evolution of peptide and protein binding properties in vitro, Curr. Opin. Biotechnol. 3, 355-362.