Protein-protein interactions in the complex between the enhancer binding protein NIFA and the sensor NIFL from Azotobacter vinelandii

Journal of Bacteriology

Volumn 183, Issue 4, 2001, Pages 1359-1368

  Money, T ^a   Barrett, J ^a   Dixon, R ^a   Austin, S ^a  

^a JOHN INNES CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; BINDING PROTEIN; PHOSPHOTRANSFERASE; PROTEIN HISTIDINE KINASE; PROTEIN NIFA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; AZOTOBACTER VINELANDII; CARBOXY TERMINAL SEQUENCE; CHROMATOGRAPHY; COMPLEX FORMATION; ENHANCER REGION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; SENSOR; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION;

ADENOSINE TRIPHOSPHATE; AZOTOBACTER VINELANDII; BACTERIAL PROTEINS; BINDING SITES; CHYMOTRYPSIN; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); MODELS, CHEMICAL; PROTEIN BINDING; SERINE ENDOPEPTIDASES; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS; TRYPSIN;

EID: 0035143627     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.4.1359-1368.2001     Document Type: Article

References (24)

1. C-terminal DNA binding stimulates N-terminal phosphorylation of the outer membrane protein regulator OmpR from Escherichia coli
2. Purification and in vitro activities of the native nitrogen fixation control proteins NIFA and NIFL
3. The isolated catalytic domain of NifA, a bacterial enhancer-binding protein, activates transcription in vitro: Activation is inhibited by NifL
4. Sequence and molecular analysis of the nifL gene of Azotobacter vinelandii
5. The oxygen-responsive NIFL-NIFA complex: A novel two-component regulatory system controlling nitrogenase synthesis in gamma-proteobacteria
6. The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae
7. Sequence of nifL from Klebsiella pneumoniae: Mode of action and relationship to two families of regulatory proteins
8. Transcriptional activation of the nitrogenase promoter in vitro: Adenosine nucleotides are required for inhibition of NIFA activity by NIFL
9. Effector-induced self-association and conformational changes in the enhancer-binding protein NTRC
10. Mechanism of translational coupling in the nifLA operon of Klebsiella pneumoniae
11. Mechanism of coordinated synthesis of the antagonistic regulatory proteins NifL and NifA of Klebsiella pneumoniae
12. Transcription termination within the regulatory nifLA operon of Klebsiella pneumoniae
13. Role of metal ions in negative regulation of nitrogen fixation by the nifL gene product from Klebsiella pneumoniae
14. Azotobacter vinelandii NIFL is a flavoprotein that modulates transcriptional activation of nitrogen-fixation genes via a redox-sensitive switch
15. Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes
16. Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes
17. Isolation and properties of the complex between the enhancer binding protein NIFA and the sensor NIFL
18. Two-domain reconstitution of a functional protein histidine kinase
19. Communication modules in bacterial signalling proteins
20. NifL of Klebsiella pneumoniae carries an N-terminally bound FAD cofactor, which is not directly required for the inhibitory function of NifL
21. Iron is required to relieve inhibitory effects of NifL on transcriptional activation by NifA in Klebsiella pneumoniae
22. The redox and fixed nitrogen-responsive regulatory protein NIFL from Azotobacter vinelandii comprises discrete flavin and nucleotide-binding domains
23. Redundancy of the conserved His residue in Azotobacter vinelandii NifL, a histidine autokinase homologue which regulates transcription of nitrogen fixation genes
24. The Q linker: A class of interdomain sequences found in bacterial multidomain regulatory proteins