Evaluation of interleukin-4 concentration by ELISA is influenced by the consumption of IL-4 by cultured cells

Journal of Interferon and Cytokine Research

Volumn 21, Issue 1, 2001, Pages 39-43

  Ewen, C ^a   Baca Estrada, M E ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 4;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOKINE RELEASE; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HELPER CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY;

ANIMALS; BINDING SITES, ANTIBODY; BINDING, COMPETITIVE; CELLS, CULTURED; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; EXOTOXINS; FEMALE; IMMUNE SERA; INTERFERON TYPE II; INTERLEUKIN-4; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; RECEPTORS, INTERLEUKIN-4; SPLEEN;

ANIMALIA;

EID: 0035135080     PISSN: 10799907     EISSN: None     Source Type: Journal    
DOI: 10.1089/107999001459141     Document Type: Article

References (23)

1. Revisiting the Th1/Th2 paradigm
2. IL-4: Role in disease and regulation of production
3. Competitive RT-PCR ELISA: A rapid, sensitive and non-radioactive method to quantitate cytokine mRNA
4. Differential spontaneous expression of mRNA for IL-4, IL-10, IL-13, IL-2 and interferon-gamma (IFN-gamma) in peripheral blood mononuclear cells (PBMC) from atopic patients
5. Quantitative polymerase chain reaction with oligodeoxynucleotide ligation assay/enzyme-linked immunosorbent assay detection
6. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations
7. The detection and enumeration of cytokine-secreting cells in mice and man and the clinical application of these assays
8. Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells
9. Incorporation of cells into an ELISA system enhances antigen-driven lymphokine detection
10. Direct comparison of ELISPOT and ELISA-based assays for detection of individual cytokine-secreting cells
11. Induction of cytokines in mice with parainfluenza pneumonia
12. Detection of IL-2 at mRNA and protein levels in synovial infiltrates from inflammatory arthropathies using biotinylated oligonucleotide probes in situ
13. Effect of IL-4 and IL-12 liposomal formulations on the induction of immune response to bovine herpesvirus type-1 glycoprotein D
14. Effects of IL-12 on immune responses induced by transcutaneous immunization with antigens formulated in a novel lipid-based biphasic delivery system
15. IL-4-dependent upregulation of IL-4 receptor expression in murine T and B cells
16. Production of soluble IL-4 receptors by murine spleen cells is regulated by T cell activation and IL-4
17. Two distinct stimulus-dependent pathways lead to production of soluble murine interleukin-4 receptor
18. Monoclonal antibodies block murine IL-4 receptor function
19. Induction of B cell activities by interleukin 4 is inhibited by a receptor-specific monoclonal antibody in vitro
20. -) thymocytes
21. Induction and regulation of IL-4 receptor expression on murine macrophage cell lines and bone marrow-derived macrophages by IFN-gamma
22. Subsets of murine lung fibroblasts express membrane-bound and soluble IL-4 receptors. Role of IL-4 in enhancing fibroblast proliferation and collagen synthesis
23. Comparison of cytokine measurements using ELISA, ELISPOT and semiquantitative RT-PCR