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note
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The centers of the Gaussians (Table 1) used for PA azurin are in very good agreement with those obtained by Solomon and co-workers.6 However, the widths of the Gaussians reported here are narrower than those in ref 6 because the absorption spectrum that we obtain of PA azurin in 50% glycerol-buffer glass (pH 8.8) at 77 K is narrower than that of the protein in a film shown in ref 6. In the course of the fitting, we obtained two separate sets of Gaussians that gave very good fits to the 77 K absorption spectrum. However, these were rejected because they did not agree with the published MCD data. Nevertheless, the |Δμ| and (Act) values obtained from fitting the electroabsorption signal using any of these three different sets of Gaussians lie within the limits of experimental error.
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33645940207
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The error margins on these measurements, which are due to the weakness of the Stark signal and the complexity of the fitting required (see below), suggest that the maximum angle between m and Aft is ~20°.
-
The error margins on these measurements, which are due to the weakness of the Stark signal and the complexity of the fitting required (see below), suggest that the maximum angle between m and Aft is ~20°.
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40
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33645899244
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Our equations differ slightly from those of ref
-
Our equations differ slightly from those of ref 15 because we report the average value of Act rather than its trace.
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