Amplification and direct sequence analysis of the 23S rRNA gene from thermophilic bacteria

BioTechniques

Volumn 30, Issue 2, 2001, Pages 414-420

  Ibrahim, A ^a   Hofman Bang, J ^a   Ahring, B K ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; EXODEOXYRIBONUCLEASE III; PARATHION; PRIMER DNA; RIBOSOME RNA; RNA 23S;

ANALYTIC METHOD; ARTICLE; DNA PURIFICATION; GENE AMPLIFICATION; GENE SEQUENCE; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; NONHUMAN; POLYMERASE CHAIN REACTION; QUALITY CONTROL; SEQUENCE ANALYSIS; THERMOPHILIC BACTERIUM;

BACTERIA (MICROORGANISMS); DECAPODA (CRUSTACEA); NEGIBACTERIA; POSIBACTERIA;

EID: 0035132147     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01302rr05     Document Type: Article

References (13)

1. The oligonuleotide probe database
2. Comparative-analysis of 23S-ribosomal RNA gene-sequences of Bacillus-anthracis and emetic Bacillus-cereus determined by PCR-direct sequencing
3. Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli
4. Database on the structure of large subunit ribosomal RNA
5. Elimination of sequence ambiguities by a single-step modification of a solidphase, single-stranded sequencing protocol
6. A new version of the RDP (Ribosomal Database Project)
7. Phylogenetic analysis of Piscirickettsia salmonis by 16S, internal transcribed spacer (ITS) and 23S ribosomal DNA sequencing
8. Heat-soaked PCR - An efficient method for DNA amplification with applications to forensic analysis
9. Comparative analysis of 16S and 23S rRNA sequences of Listeria species
10. The EMBL nucleotide sequence database
11. Amplification and sequencing of variable regions in bacterial 23S ribosomal-RNA genes with conserved primer sequences
12. The universal ancestor
13. Rapid distinction between Leptospiral interrogans and Leptospira biflexa by PCR amplification of 23S ribosomal DNA