Elimination of sequence ambiguities by a single-step modification of a solid-phase, single-stranded sequencing protocol

BioTechniques

Volumn 21, Issue 3, 1996, Pages 356-358

  Lawson, Victoria Alice ^a   McPhee, Dale Alan ^a   Deacon, Nicholas John ^a  

^a MACFARLANE BURNET INSTITUTE FOR MEDICAL RESEARCH AND PUBLIC HEALTH   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; SINGLE STRANDED DNA;

ARTICLE; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION;

EID: 0029810989     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96213bm01     Document Type: Article

References (7)

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2. Bachman, B., W. Lüke and G. Hunsmann. 1990. Improvement of PCR amplified sequencing with the aid of detergents. Nucleic Acids Res. 18:1309.
3. Deacon, N.J., A. Tyskin, A. Solomon, K. Smith, M. Ludford-Menting, D.J. Hooker, D.A. McPhee, A.L. Greenway, et al. 1995. Genomic structure of an attenuated quasi species of HIV-1 from a blood transfusion donor and recipients. Science 270:988-991.
4. Hultman, T., S. Ståhl, E. Hornes and M. Uhlén. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res. 17:4937-4946.
5. Mitchell, L.G. and C.R. Merril. 1989. Affinity generation of single-stranded DNA for dideoxy sequencing following the polymerase chain reaction. Anal. Biochem. 178:239-242.
6. Mitchell, L.G., R. Vaghmar and J.J. Mutagh, Jr. 1994. Biotin-enhanced sequencing of PCR products and generation of single-stranded DNA probes, p. 31-48. In M. Uhlen, B. Hornes and Ø. Olsirik (Eds.), Advances in Biomagnetic Separation. Eaton Publishing, Natick, MA.
7. Myer, G., B. Korber, J.A. Berzofsky, R.F. Smith and G.N. Pavlakis. 1992. Human Retroviruses and AIDS. Theoretical Biology and Biophysics. Los Alamos National Laboratory, Los Alamos.