Transgenic mice for the preparation of puromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selection

Mammalian Genome

Volumn 12, Issue 2, 2001, Pages 169-171

  Linnell, Elizabeth R ^a   Lerner, Charles P ^b   Johnson, Kevin A ^b   Leach, Craig A ^b   Ulrich, Thomas R ^b   Rafferty, William C ^b   Simpson, Elizabeth M ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b JACKSON LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PUROMYCIN;

ANIMAL CELL; ARTICLE; DRUG TARGETING; EMBRYO; FIBROBLAST; GENE EXPRESSION; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TRANSGENE; TRANSGENIC MOUSE;

ANIMALS; CELL LINE; DRUG RESISTANCE; FEMALE; FIBROBLASTS; MALE; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; PLASMIDS; PUROMYCIN; SELECTION (GENETICS); STEM CELLS;

ANIMALIA; MUS MUSCULUS; RODENTIA;

EID: 0035124928     PISSN: 09388990     EISSN: None     Source Type: Journal    
DOI: 10.1007/s003350010238     Document Type: Article

References (12)

1. R in transgenic mice causes resistance to toxic effects of hygromycin-B in vivo
2. Inter-chromosomal recombination of Mll and Af9 genes mediated by cre-loxP in mouse development
3. Pac gene as efficient dominant marker and reporter gene in mammalian cells
4. Use of puromycin N-acetyltransferase (PAC) as a new reporter gene in transgenic animals
5. Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblasts feeder layers for embryonic stem cell selection
6. Murine PGK-1 promoter drives widespread but not uniform expression in transgenic mice
7. A series of mammalian expression vectors and characterisation of their expression of a receptor gene in stably and transiently transfected cells
8. Efficient production of Cre-mediated site-directed recombinants through the utilization of the puromycin resistance gene, pac: A transient gene-integration marker for ES cells
9. Germ-line passage is required for establishment of methylation and expression patterns of imprinted but not of nonimprinted genes
10. A transgenic mouse strain expressing four drug-selectable marker genes
11. Stable production of mutant mice from double gene converted ES cells with puromycin and neomycin
12. Expression of a mouse Zfy-1/lacZ transgene in the somatic cells of the embryonic gonad and germ cells of the adult testis