Multiplex PCR by multicolor fluorimetry and fluorescence melting curve analysis

Nature Medicine

Volumn 7, Issue 2, 2001, Pages 249-253

  Elenitoba Johnson, Kojo S J ^a,b   Bohling, Sandra D ^b   Wittwer, Carl T ^a,b   King, Thomas C ^c  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b ARUP INSTITUTE FOR CLINICAL AND EXPERIMENTAL PATHOLOGY   (United States)

^c BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ARTICLE; DNA SEQUENCE; FLUOROMETRY; GENE AMPLIFICATION; HOMOZYGOSITY; HUMAN; HUMAN CELL; MELTING POINT; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BASE SEQUENCE; COLOR; FLUORESCENCE; FLUOROMETRY; GENES, RAS; HL-60 CELLS; HUMANS; LEUKEMIA, MYELOCYTIC, ACUTE; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TEMPERATURE; TUMOR CELLS, CULTURED;

EID: 0035122455     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/84708     Document Type: Article

References (22)

1. Continuous fluorescence monitoring of rapid cycle DNA amplification
2. Integrated amplification and detection of the C677T point mutation in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves
3. Real-time quantitative PCR
4. Spectral genotyping of human alleles
5. Molecular beacons: Probes that fluoresce upon hybridization
6. Digital PCR
7. Detection of the hereditary hemochromatosis gene mutation by real-time fluorescence polymerase chain reaction and peptide nucleic acid clamping
8. Chemiluminescent and fluorescent probes for DNA hybridization systems
9. Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer
10. Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes
11. Seven-color, homogeneous detection of six PCR products
12. Multicolor molecular beacons for allele discrimination
13. A general method of site-specific mutagenesis using a modification of the Thermus aquaticus polymerase chain reaction
14. Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR
15. Color multiplexing hybridization probes using the apolipoprotein E locus as a model system for genotyping
16. Fluorescence spectral overlap compensation for any number of flow cytometry parameters
17. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction
18. Nucleic acid hybrids, formation and structure
19. Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs
20. Improved nearest-neighbor parameters for predicting DNA duplex stability
21. Nearest-neighbor thermodynamics and NMR of DNA sequences with internal A.A, C.C, G.G, and T.T mismatches
22. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest- neighbor thermodynamics