Targeted inactivation of the mecB gene, encoding cystathionine-γ-lyase, shows that the reverse transsulfuration pathway is required for high-level cephalosporin biosynthesis in Acremonium chrysogenum C10 but not for methionine induction of the cephalosporin genes

Journal of Bacteriology

Volumn 183, Issue 5, 2001, Pages 1765-1772

  Liu, G ^a   Casqueiro, J ^a   Banuelos O ^a   Cardoza, R E ^a   Gutierrez S ^a   Martin J F ^a  

^a UNIVERSITY OF LEÓN   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CEPHALOSPORIN; CYSTATHIONINE GAMMA LYASE; METHIONINE;

ACREMONIUM; ARTICLE; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME ACTIVITY; GENE DISRUPTION; GENE INACTIVATION; GENE INDUCTION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SOUTHERN BLOTTING; STRAIN DIFFERENCE; SULFATION; TECHNIQUE;

ACREMONIUM; ANTI-BACTERIAL AGENTS; BLOTTING, SOUTHERN; CEPHALOSPORINS; CINNAMATES; CYSTATHIONINE GAMMA-LYASE; DRUG RESISTANCE, MICROBIAL; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENE TARGETING; HYGROMYCIN B; METHIONINE; PHLEOMYCINS; SULFUR; TRANSFORMATION, GENETIC;

ACREMONIUM CHRYSOGENUM; FUNGI;

EID: 0035117592     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.5.1765-1772.2001     Document Type: Article

References (39)

1. Penicillin and cephalosporin biosynthetic genes: Structure, organization, regulation and evolution
2. Transformation by integration in Podospora anserina. I. Methodology and phenomenology
3. Role of methionine in cephalosporin synthesis
4. Intrachromosomal recombination between direct repeats in Penicillium chrysogenum: Gene conversion and deletion events
5. Gene targeting in Penicillium chrysogenum: Disruption of the lys2 gene leads to penicillin overproduction
6. Strain exchange between industry and academia
7. Biosynthesis of cephalosporins
8. Effect of primary metabolites on secondary metabolism
9. Transformation in fungi
10. A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids
11. Targeted transformation of Ascobolus inmersus and de novo methylation of the resulting duplicated sequences
12. Characterization of the Cephalosporium acremonium pcbAB gene encoding α-amino-adipyl-cysteinil-valine synthetase, a large multidomain peptide synthetase: Linkage to the pcbC gene as a cluster of early cephalosporin biosynthetic genes and evidence of multiple functional domains
13. Transcription of the pcbAB, pcbC and penDE genes of Penicillium chrysogenum AS-P-78 is repressed by glucose and the repression is not reversed by alkaline pHs
14. Gene disruption of the pcbAB gene encoding ACV synthetase in Cephalosporium acremonium
15. Mitotic stability of transforming DNA is determined by its chromosomal configuration in the fungus Cochiobolus heterostrophus
16. Effect of methionine on cephalosporin C and penicillin N production by a mutant of Cephalosporium acremonium
17. Sulphate and methionine as sulphur sources for cysteine and cephalosporin C synthesis in Cephalosporium acremonium
18. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: A general strategy for targeting mutations to non-selectable genes
19. Characterization of the reverse transsulfuration mecB gene of Acremonium chrysogenum encoding a functional cysthationine-γ-lyase
20. Regulation of the biosynthesis of β-lactam antibiotics
21. Penicillin and cephalosporin biosynthesis: Mechanism of carbon catabolite regulation of penicillin production
22. β-Lactams
23. The biosynthesis of sulfur-containing β-lactam antibiotics
24. The biosynthesis of cephalosporin C
25. Cysteine biosynthesis in Saccharomyces cerevisiae: A new outlook on pathway and regulation
26. Sulphur metabolism
27. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli
28. Coordinate increase of isopenicillin N synthetase, isopenicillin N epimerase and deacetoxycephalosporin C synthetase in a high cephalosporin-producing mutant of A. chrysogenum and simultaneous loss of the three enzymes in a non-producing mutant
29. Increase in activity of β-lactam synthetases after growth of Cephalosporium acremonium with methionine or norleucine
30. Intermediates in inorganic sulphate utilization by Penicillium chrysogenum
31. Levels of isopenicillin N synthetase and deacetoxycephalosporin C synthetase in Cephalosporium acremonium producing high and low levels of cephalosporin C
32. Transformation by integration in Aspergillus nidulans
33. Role of sulfur metabolism in cephalosporin C and penicillin biosynthesis
34. Exogenous methionine increases levels of mRNAs transcribed from pcbAB, pcbC, and cefEF genes, encoding enzymes of the cephalosporin biosynthetic pathway in Acremonium chrysogenum
35. Targeted integration into the Acremonium chrysogenum genome: Disruption of the pcbC gene
36. Transformation of Aspergillus nidulans by using a trpC plasmid
37. Methionine induction of ACV synthetase in Cephalosporium acremonium