The Escherichia coli SeqA protein binds specifically to two sites in fully and hemimethylated oriC and has the capacity to inhibit DNA replication and affect chromosome topology

Biochimie

Volumn 83, Issue 1, 2001, Pages 49-51

  Skarstad, Kirsten ^a   Torheim, Norunn ^a   Wold, Sture ^a   Lurz, Rudi ^b   Messer, Walter ^b   Fossum, Solveig ^a   Bach, Trond ^a  

^a INSTITUTE OF CANCER RESEARCH   (Norway)

^b MAX PLANCK INSTITUTE FOR MOLECULAR GENETICS   (Germany)

Author keywords

DnaA; Electron microscopy; In vitro replication; Initiation of DNA replication; SeqA; Supercoiling

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL DNA; DNA BINDING PROTEIN;

ARTICLE; BINDING SITE; CHROMOSOME MAP; DNA BINDING; DNA METHYLATION; DNA REPLICATION; DNA REPLICATION ORIGIN; DNA SUPERCOILING; DNA SYNTHESIS; DNA SYNTHESIS INHIBITION; ELECTRON MICROSCOPY; ESCHERICHIA COLI; NONHUMAN; PROTEIN DNA INTERACTION;

ESCHERICHIA COLI;

EID: 0035116562     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(00)01207-4     Document Type: Article

References (15)

1. Hemimethylation prevents DNA replication in E. coli
2. SeqA: A negative modulator of replication initiation in E. coli
3. SeqA limits DnaA activity in replication from oriC in Escherichia coli
4. E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration
5. A protein that binds to the P1 origin core and the oriC 13mer region in a methylation-specific fashion is the product of the host seqA gene
6. Binding of SeqA protein to DNA requires interaction between two or more complexes bound to separate hemimethylated GATC sequences
7. Coordinating DNA replication initiation with cell growth: Differential roles for DnaA and SeqA proteins
8. Escherichia coli SeqA protein affects DNA topology and inhibits open complex formation at oriC
9. Effects of purified SeqA protein on oriC-dependent DNA replication in vitro
10. Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli
11. Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli
12. The Escherichia coli SeqA protein binds specifically and cooperatively to two sites in hemimethylated and fully methylated oriC
13. DnaA protein binding to individual DnaA boxes in the Escherichia coli replication origin, oriC
14. Cell cycle-specific changes in nucleoprotein complexes at a chromosomal replication origin
15. The escherichia coli SeqA protein destabilizes mutant DnaA204 protein