A general method for the quantitative analysis of functional chimeras: Applications from site-directed mutagenesis and macromolecular association

Protein Science

Volumn 10, Issue 3, 2001, Pages 581-591

  Luong, Tinh N ^a,b   Kirsch, Jack F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Sunesis Pharmaceuticals Inc   (United States)

Author keywords

Aminotransferase; Chimera; Context dependence; Macromolecular interactions; Oncomodulin; Protein genetic engineering; Tat TAR

Indexed keywords

ASPARTIC ACID; DICARBOXYLIC ACID; ONCOMODULIN; TYROSINE AMINOTRANSFERASE;

ARTICLE; CALCIUM TRANSPORT; CALCULATION; CHIMERA; ENERGY; ENZYME PURIFICATION; GENE MUTATION; GENETIC ENGINEERING; KINETICS; MACROMOLECULE; PRIORITY JOURNAL; PROTEIN BINDING; QUANTITATIVE DIAGNOSIS; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ASPARTATE AMINOTRANSFERASES; CALCIUM-BINDING PROTEINS; ESCHERICHIA COLI; KINETICS; MACROMOLECULAR SUBSTANCES; MUTAGENESIS, SITE-DIRECTED; QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATION (GENETICS); TYROSINE TRANSAMINASE;

BOVINAE;

EID: 0035106702     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.24101     Document Type: Article

References (11)

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2. Escherichia coli aromatic amino acid aminotransferase: Characterization and comparison with aspartate aminotransferase
3. 2+-binding affinity
4. Reversible dissociation and unfolding of aspartate aminotransferase from Escherichia coli: Characterization of a monomeric intermediate
5. Strategy for analysing the co-operativity of intramolecular interactions in peptides and proteins
6. Alternating arginine-modulated substrate specificity in an engineered tyrosine aminotransferase
7. A continuous coupled spectrophotometric assay for tyrosine aminotransferase activity with aromatic and other nonpolar amino acids
8. Redesign of the substrate specificity of Escherichia coli aspartate aminotransferase to that of Escherichia coli tyrosine aminotransferase by homology modeling and site-directed mutagenesis
9. Energetic analysis of an antigen/ antibody interface: Alanine scanning mutagenesis and double mutant cycles in the HyHEL-10/lysozyme interaction
10. Altering the context of an RNA bulge switches the binding specificities of two viral Tat proteins
11. Additivity of mutational effects in proteins