In vitro assays of vesicular transport

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Volumn 2, Issue 1, 2001, Pages 19-25

  Cook, N R ^a   Davidson, H W ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Broken cell; Cell free; Membrane fusion; Vesicle budding; Vesicular transport

Indexed keywords

LIPID; PROTEIN;

ANIMAL CELL; ARTICLE; ASSAY; CELL COMPARTMENTALIZATION; CELL FRACTIONATION; CELL TRANSPORT; CELL VACUOLE; CHO CELL; CONTROLLED STUDY; CYTOLOGY; ELECTRON MICROSCOPY; ENDOCYTOSIS; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; EUKARYOTIC CELL; EXOCYTOSIS; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; GOLGI COMPLEX; HOMEOSTASIS; IN VITRO STUDY; LIPID TRANSPORT; LYSOSOME; MAMMAL CELL; MOLECULAR BIOLOGY; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; YEAST CELL;

ANIMALS; BIOCHEMISTRY; BUFFERS; CELL FRACTIONATION; INTRACELLULAR MEMBRANES; MEMBRANE FUSION; PROTEIN TRANSPORT; TRANSPORT VESICLES;

EID: 0035102571     PISSN: 13989219     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0854.2001.020104.x     Document Type: Article

References (83)

1. Semi-intact cells permeable to macromolecules: Use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex
2. Perforated MDCK cells support intracellular transport
3. Morphological analysis of protein transport from the ER to Golgi membranes in digitonin-permeabilized cells: Role of the p58 containing compartment
4. COPII vesicles derived from mammalian endoplasmic reticulum microsomes recruit COPI
5. Reconstitution of transport of vesicular stomatitis virus G protein from the endoplasmic reticulum to the Golgi complex using a cell-free system
6. Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglu-cosamine
7. Transport of the vesicular stomatitis glycoprotein to trans Golgi membranes in a cell-free system
8. Cell-free transport to distinct Golgi cisternae is compartment specific and ARF independent
9. Transport of vesicular stomatitis virus glycoprotein in a cell-free
10. A cell-free assay for the insertion of a viral glycoprotein into the plasma membrane
11. Dissection of Semliki Forest virus glycoprotein delivery from the trans-Golgi network to the cell surface in permeabilized BHK cells
12. Basolateral protein transport in streptolysin O-permeabilized MDCK cells
13. Reconstitution of constitutive secretion using semi-intact cells: Regulation by GTP but not calcium
14. Cell-free reconstitution of the transport of viral glycoproteins from the TGN to the basolateral plasma membrane of MDCK cells
15. Fusion between rat pancreatic zymogen granules and plasma membranes. Modulation by a GTP-binding protein
16. 2+-activated secretion in GH3 pituitary cells
17. Neurotransmitter release from semi-intact synaptosomes
18. A major role for protein kinase C in calcium-activated exocytosis in permeabilised adrenal chromaffin cells
19. A cell-free system for regulated exocytosis in PC12 cells
20. 2+-activated exocytosis in a cell-free system
21. Plasma membrane-derived vesicles containing receptor-ligand complexes are fusogenic with early endosomes in a cell-free system
22. Isolation of functional, coated, endocytic vesicles
23. Cytoplasmic dynein-dependent vesicular transport from early to late endosomes
24. In vitro fusion of tissue-derived endosomes and lysosomes
25. Selective recycling of the mannose 6-phosphate /IGF-II receptor to the trans Golgi network in vitro
26. A novel assay reveals a role for soluble N-ethylmaleimide-sensitive fusion attachment protein in mannose 6-phosphate receptor transport from endosomes to the trans Golgi network
27. Lysosomes can fuse with a late endosomal compartment in a cell-free system
28. Fusion of lysosomes with late endosomes produces a hybrid organelle of intermediate density and is NSF dependent
29. Syntaxin 7 and VAMP-7 are soluble N-ethylmaleimide-sensitive factor attachment protein receptors required for late endosome-lysosome and homotypic lysosome fusion in alveolar macrophages
30. ATP and cytosol requirements for transferrin recycling in intact and disrupted MDCK cells
31. In vitro fusion of phagosomes with different endocytic organelles from J774 macrophages
32. Fusion of newly formed phagosomes with endosomes in intact cells and in a cell-free system
33. Reconstitution of phagosome-lysosome fusion in streptolysin O-permeabilized cells
34. Control of protein traffic between distinct plasma membrane domains. Requirement for a novel 108000 protein in the fusion of transcytotic vesicles with the apical plasma membrane
35. Reconstitution of transcytosis in SLO-permeabilized MDCK cells: Existence of an NSF-dependent fusion mechanism with the apical surface of MDCK cells
36. Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: The acceptor Golgi compartment is defective in the sec23 mutant
37. Reconstitution of SEC gene product-dependent intercompartmental protein transport
38. Reconstitution of retrograde transport from the Golgi to the ER in vitro
39. In vitro reconstitution of intercompartmental protein transport to the yeast vacuole
40. A cell-free assay allows reconstitution of Vps33p-dependent transport to the yeast vacuole lysosome
41. Compartmental organization of the Golgi stack
42. Deficient uridine diphosphate-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase activity in a clone of Chinese hamster ovary cells with altered surface glycoproteins
43. Reconstitution of an endocytic fusion event in a cell-free system
44. Fusion between endocytic vesicles in a cell-free system
45. Detection of endocytic vesicle fusion in vitro. Using assay based on avidin-biotin association reaction
46. Fusion between vesicles from the pathway of receptor-mediated endocytosis in a cell-free system
47. Reassembly of Golgi stacks from mitotic Golgi fragments in a cell-free system
48. Oligomerization of immunoglobulin G heavy and light chains in vitro. A cell-free assay to study the assembly of the endoplasmic reticulum
49. Proteolytic cleavage of haptoglobin occurs in a subcompartment of the endoplasmic reticulum: Evidence from membrane fusion in vitro
50. The karyogamy gene KAR2 and novel proteins are required for ER-membrane fusion
51. Reconstitution of vesiculated Golgi membranes into stacks of cisternae: Requirement of NSF in stack formation
52. Homotypic fusion of immature secretory granules during maturation in a cell-free assay
53. In vitro fusion of endosomes following receptor-mediated endocytosis
54. Reconstitution of vesicle fusions occurring in endocytosis with a cell-free system
55. Microtubule-and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells
56. Homotypic lysosome fusion in macrophages: Analysis using an in vitro assay
57. In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae
58. Characterization of protein transport between successive compartments of the Golgi apparatus: Asymmetric properties of donor and acceptor activities in a cell-free system
59. Reconstitution of clathrin-coated pit budding from plasma membranes
60. Release of putative exocytic transport vesicles from perforated MDCK cells
61. Intermediates in the constitutive and regulated secretory pathways released in vitro from semi-intact cells
62. Prohormone processing in the trans-Golgi network: Endoproteolytic cleavage of prosomatostatin and formation of nascent secretory vesicles in permeabilized cells
63. Large dense-core vesicle exocytosis in PC12 cells
64. Differential inhibition of multiple vesicular transport steps between the endoplasmic reticulum and trans Golgi network
65. Distinct biochemical requirements for the budding, targeting, and fusion of ER-derived transport vesicles
66. Formation of coated vesicles from coated pits in broken A431 cells
67. COP-coated vesicles are involved in the mitotic fragmentation of Golgi stacks in a cell-free system
68. Stage-specific assays for coated pitformation and coated vesicle budding in vitro
69. Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast
70. Isolation of functional Golgi-derived vesicles with a possible role in retrograde transport
71. Stepwise assembly of functionally active transport vesicles
72. The in vitro generation of post-Golgi vesicles carrying viral envelope glycoproteins requires an ARF-like GTP-binding protein and a protein kinase C associated with the Golgi apparatus
73. Cell-free protein sorting to the regulated and constitutive secretory pathways
74. Exocytic transport vesicles generated in vitro from the trans-Golgi network carry secretory and plasma membrane proteins
75. Isolation of exocytic carrier vesicles from BHK cells
76. Biogenesis of synaptic vesicles in vitro
77. Calcium and GTP: Essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus
78. Reconstitution of endosomal proteolysis in a cell-free system. Transfer of immune complexes internalized via Fc receptors to an endosomal proteolytic compartment
79. COPII: A membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum
80. Yeast and mammals utilise similar cytosolic components to drive protein transport through the Golgi complex
81. Involvement of ezrin moesin in de novo actin assembly on phagosomal membranes
82. Protein sorting by transport vesicles
83. Coat assembly directs v-SNARE concentration into synthetic COPII vesicles