Sialic acid-dependent binding of baculovirus-expressed recombinant antigens from Plasmodium falciparum EBA-175 to glycophorin A

Molecular and Biochemical Parasitology

Volumn 113, Issue 1, 2001, Pages 9-21

  Ockenhouse, Christian F ^a   Barbosa, Arnoldo ^a   Blackall, Douglas P ^b   Murphy, Cheryl I ^c   Kashala, Oscar ^c   Dutta, Sheetij ^a   Lanar, David E ^a   Daugherty, Jon R ^a  

^a WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

^b UCLA MEDICAL CENTER   (United States)

^c Aquila Biopharmaceuticals Inc   (United States)

Author keywords

Baculovirus expression; EBA 175; Glycophorin A; Invasion; Malaria

Indexed keywords

ANTIGEN; CYSTEINE; ERYTHROCYTE BINDING ANTIGEN 175; GLYCOPHORIN A; SIALIC ACID; SYNTHETIC PEPTIDE; TRYPSIN; UNCLASSIFIED DRUG; VACCINE;

ANIMAL CELL; ANTIGEN BINDING; ANTIGEN EXPRESSION; ANTIGENICITY; ARTICLE; BACULOVIRUS; CELL CULTURE; CONTROLLED STUDY; ERYTHROCYTE; IMMUNOGENICITY; MALARIA; MEROZOITE; MOLECULAR RECOGNITION; NONHUMAN; PLASMODIUM FALCIPARUM; PRIORITY JOURNAL; PROTEIN DOMAIN;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, PROTOZOAN; BACULOVIRIDAE; CARRIER PROTEINS; ERYTHROCYTES; GENETIC VECTORS; GLYCOPHORIN; HUMANS; IMMUNE SERA; MICE; MOLECULAR SEQUENCE DATA; N-ACETYLNEURAMINIC ACID; NEURAMINIDASE; PEPTIDE FRAGMENTS; PEPTIDES; PLASMODIUM FALCIPARUM; PROTEIN BINDING; PROTOZOAN PROTEINS; RABBITS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; TRYPSIN;

INSECTA; PLASMODIUM FALCIPARUM; UNIDENTIFIED BACULOVIRUS;

EID: 0035092082     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(00)00375-3     Document Type: Article

References (27)

1. A Plasmodium falciparum antigen that binds to host erythrocytes and merozoites
2. A malaria invasion receptor, the 175-kilodalton erythrocyte binding antigen of Plasmodium falciparum recognizes the terminal Neu5Ac(alpha 2-3)Gal-sequences of glycophorin A
3. Primary structure of the 175K Plasmodium falciparum erythrocyte binding antigen and identification of a peptide which elicits antibodies that inhibit malaria merozoite invasion
4. A family of erythrocyte binding proteins of malaria parasites
5. Cloning the P. falciparum gene encoding PfEMP1, a malarial variant antigen and adherence receptor on the surface of parasitized human erythrocytes
6. Switches in expression of Plasmodium falciparum var genes correlate with changes in antigenic and cytoadherent phenotypes of infected erythrocytes
7. The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of Plasmodium falciparum-infected erythrocytes
8. Conservation of structure and function of the erythrocyte-binding domain of Plasmodium falciparum EBA-175
9. Two alleles of the 175-kilodalton Plasmodium falciparum erythrocyte binding antigen
10. Receptor and ligand domains for invasion of erythrocytes by Plasmodium falciparum
11. Identification of the erythrocyte binding domains of Plasmodium vivax and Plasmodium knowlesi proteins involved in erythrocyte invasion
12. Expression, purification and characterization of a functional region of the Plasmodium vivax Duffy binding protein
13. Baculovirus-mediated expression of Plasmodium falciparum erythrocyte binding antigen 175 polypeptides and their recognition by human antibodies
14. Human malaria parasites in continuous culture
15. The baculovirus expression vector pBSV-8His directs secretion of histidine-tagged proteins
16. General method for the rapid solid-phase synthesis of large numbers of peptides: Specificity of antigen-antibody interaction at the level of individual amino acids
17. Isolation of glycoproteins from red blood cell membranes using pyridine
18. Targeted gene disruption shows that knobs enable malaria-infected red cells to cytoadhere under physiological shear stress
19. Functional conservation of the malaria vaccine antigen MSP-1 19 across distantly related Plasmodium species
20. Targeted disruption of an erythrocyte binding antigen in Plasmodium falciparum is associated with a switch toward a sialic acid-independent pathway of invasion
21. A recombinant baculovirus-expressed Plasmodium falciparum receptor-binding domain of erythrocyte binding protein EBA-175 biologically mimics native protein
22. The role of sialic acid in the expression human MN blood group antigens
23. Plasmodium falciparum field isolates commonly use erythrocyte invasion pathways that are independent of sialic acid residues of glycophorin A
24. Antibodies against the Plasmodium falciparum receptor binding domain of EBA-175 block invasion pathways that do not involve sialic acids
25. Glycophorin B as an EBA-175 independent Plasmodium falciparum receptor of human erythrocytes
26. Falciparum malaria parasites invade erythrocytes that lack glycophorin A and B (MkMk). Strain differences indicate receptor heterogeneity and two pathways for invasion