Expression of the EPO-like domains of human thrombopoietin in Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 8, Issue 6, 1998, Pages 553-559

  Koh, Yeo Wook ^a   Koo, Tai Young ^a   Ju, Sang Myoung ^a   Kwon, Chang Hyuk ^a   Chung, Joo Young ^a   Park, Myung Hwan ^a   Yang, Jai Myung ^a   Park, Seung Kook ^a  

^a Daewoong Pharmaceutical Co Ltd   (South Korea)

Author keywords

E. coli; EPO like domain; Expression; Human thrombopoietin

Indexed keywords

THIOREDOXIN; THROMBOPOIETIN;

ARTICLE; BONE MARROW TRANSPLANTATION; DNA LIBRARY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; LIGAND BINDING; MEGAKARYOCYTE; MOLECULAR CLONING; OPEN READING FRAME; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN EXPRESSION;

ESCHERICHIA COLI;

EID: 0032446212     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Bartley, T. D., J. Bogenberger, P. Hunt, Y. S. Li, H. S. Lu, F. Martin, M. S. Chang, B. Samal, J. L. Nichol, S. Swift, M. J. Johnson, R. Y. Hsu, V. P. Parker, S. Suggs, J. D. Skrine, L. A. Merewether, C. Clogston, E. Hsu, M. M. Hokom, A. Hornkohl, E. Choi, M. Pangelinan, Y. Sun, V. Mar, J. McNinch, L. Simonet, F. Jacobsen, C. Xie, J. Shutter, H. Chute, R. Basu, L. Selander, D. Trollinger, L. Sieu, D. Padilla, G. Trail, G. Elliott, R. Izumi, T. Covery, H. Crouse, A. Garcia, W. Xu, J. Del Castillo, J. Biron, S. Cole, C. T. Hu, R. Pacifici, I. Ponting, C. Saris, D. Wen, P. Yung, H. Lin, and R. A. Bosselman. 1994. Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor mpl. Cell 77: 1117-1124.
2. de Sauvage, F. J., P. E. Hass, S. D. Spencer, B. E. Malloy, A. L. Gurney, S. A. Spencer, W. C. Darbonne, W. J. Henzel, S. C. Wong, W. Kuang, K. J. Oles, B. Hutgren, L. A. Solberg Jr, D. Goeddel, and D. L. Eaton. 1994. Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-mpl ligand. Nature 369: 533-538.
3. Eaton, D. L. and F. J. de Sauvage. 1997. Thrombopoietin: The primary regulator of megakaryocytopoiesis and thrombopoiesis. Exp. Hematol. 25: 1-7.
4. Foster, D. C., C. A. Spencer, F. J. Grant, J. M. Kramer, J. L. Kujiper, R. D. Holly, T. E. Whitmore, M. D. Heipel, L. A. Bell, A. F. T. Ching, N. K. McGrane, C. Hart, P. J. O'Hara, and S. Lok. 1994. Human thrombopoietin: Gene structure, cDNA sequence, expression, and chromosomal localization. Proc. Natl. Acad. Sci. USA 91: 13023-13027.
5. Hussain, K., C. Bowler, L. M. Robert, and J. M. Lord. 1989. Expression of ricin B chain in Escherichia coli. FEBS Lett. 244: 383-387.
6. Jiang, X., S. Li, A. Zhou, F. Li, X. Xu, and D. Zhu. 1996. Translation initiation region plays an important role in the expression of human thrombopoietin in Escherichia coli. Biochem. Molecul. Biol. Inter. 39: 1109-1113.
7. Lavallie, E. R., E. A. Diblasio, S. Kovacic, K. Grant, P. F. Schudel, and J. M. McCoy. 1993. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/technology 11: 187-193.
8. Lok, S. and D. C. Foster. 1994. The structure, biology, and potential therapeutic applications of recombinant thrombopoietin. Stem Cells 12: 586-598.
9. Lok, S., K. Kaushansky, R. D. Holly, J. L. Kuijper, C. E. Lofton-Day, P. J. Oort, F. J. Grant, M. D. Heipel, S. K. Burkhead, J. M. Kramer, L. A. Bell, C. A. Sprecher, H. Blumberg, R. Honson, D. Prunkard, A. F. T. Ching, S. L. Mathewes, M. C. Bailey, J. W. Forstrom, M. Buddle, S. G. Osborn, S. J. Evans, P. O. Sheppard, S. R. Presnell, P. J. O'Hara, F. S. Hagen, G. Roth, and D. C. Foster. 1994. Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo. Nature 369: 565-568.
10. Lunn, C. A., S. Kathju, B. J. Wallace, S. R. Kushner, and V. Pigiet. 1984. Amplification and purification of plasmid-encoded thioredoxin from Escherichia coli K12. J. Biol. Chem. 259: 10469-10474.
11. Makoff, A. Z., M. D. Oxer, M. A. Romanos, N. F. Fairweather, and S. Ballantine. 1989. Expression of tetanus toxin fragment C in E. coli: High level expression by removing rare codons. Nucl. Acids Res. 17: 10191-10202.
12. Maywald F., T. Boldcke, G. Gross, R. Frank, H. Blocker, A. Meyerhans, K. Schwellnus, J. Ebbers, W. Bruns, and G. Reinhardt. 1988. Human pancreatic secretory trypsin inhibitor (PSTI) in active form and secreted from Escherichia coli. Gene 68: 357-369.
13. Middelberg, A. P. J. 1996. Large-scale recovery of recombinant protein inclusion bodies expressed in Escherichia coli. J. Microbiol. Biotechnol. 6: 225-231.
14. Park, S. K., K. M. Lee, K. S. Nho, Y. W. Koh, C. H. Kwon, J. Y. Chung, Y. S. Jee, and Y. H. Yu. 1997. Gene coding human epidermal growth factor and process for preparing the same. United States Patent No. 5,652,120.
15. Parker, K. C. and D. C. Wiley. 1989. Overexpression of native human beta2-microglobulin in Escherichia coli and its purification. Gene 83: 117-124.
16. Ray, P., F. J. Moy, G. T. Montelione, J. F. Liu, S. A. Narang, H. A. Scheraga, and R. Wu. 1988. Structure-function studies of murine epidermal growth factor: Expression and site-directed mutagenesis of epidermal growth factor gene. Biochemistry 27: 7289-7295.