A novel transposon tagging element for obtaining gain-of-function mutants based on a self-stabilizing Ac derivative

Plant Molecular Biology

Volumn 45, Issue 2, 2001, Pages 123-131

  Suzuki, Yoshihito ^a   Uemura, Satoko ^a   Saito, Yuko ^a   Murofushi, Noboru ^a   Schmitz, Gregor ^b   Theres, Klaus ^b   Yamaguchi, Isomaro ^c  

^a UNIVERSITY OF TOKYO   (Japan)

^b MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH   (Germany)

^c NONE

Author keywords

Ac transposon; Activation tagging; Ds element

Indexed keywords

ENHANCER SEQUENCE; GENE MUTATION; GENE TAGGING; GENETIC STABILITY; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TANDEM REPEAT; TOBACCO; TRANSACTIVATION; TRANSPOSABLE ELEMENT; TRANSPOSON TAGGING;

BASE SEQUENCE; BLOTTING, SOUTHERN; CAULIMOVIRUS; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DNA, PLANT; DNA, RECOMBINANT; ENHANCER ELEMENTS (GENETICS); MUTAGENESIS, INSERTIONAL; MUTATION; PLANTS, TOXIC; PROMOTER REGIONS (GENETICS); SEQUENCE HOMOLOGY, NUCLEIC ACID; TOBACCO; TRANS-ACTIVATION (GENETICS); TRANSFORMATION, GENETIC;

EMBRYOPHYTA; NICOTIANA TABACUM;

EID: 0035089820     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006455130098     Document Type: Article

References (23)

1. Transposition of the maize controlling element 'Activator' in tobacco
2. The presence of enhancers adjacent to the Ac promoter increases the abundance of transposase mRNA and alters the timing of Ds excision in Arabidopsis
3. New plant binary vectors with selectable markers located proximal to the left T-DNA border
4. T-DNA insertion mutagenesis in Arabidopsis: Mutational spectrum
5. Gametophyte-specific transposition of the maize Ds element in transgenic tobacco
6. High rates of Ac/Ds germinal transposition in Arabidopsis suitable for gene isolation by insertional mutagenesis
7. Relative strengths of the 35S cauliflower mosaic virus 1′, 2′ and nopaline synthase promoters in transformed tobacco sugar beet and oilseed raped callus tissue
8. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid
9. A simple and general method for transferring genes into plants
10. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants
11. CKII, a histidine kinase homolog implicated in cytokinin signal transduction
12. T-DNA insertional mutagenesis in Arabidopsis
13. Somatic and germinal activities of maize Activator (Ac) transposase mutants in transgenic tobacco
14. Modification of the 5′ untranslated leader region of the maize Activator element leads to increased activity in Arabidopsis
15. The DNA sequence of the transposable element Ac of Zea mays L.
16. Comparative analysis of non-random DNA repair following Ac transposon excision in maize and Arabidopsis
17. A self-stabilizing Ac derivative and its potential for transposon tagging
18. Chloroplast targeting of spectinomycin adenyltransferase provides a cell-autonomous marker for monitoring transposon excision in tomato and tobacco
19. Elevated levels of Activator transposase mRNA are associated with high frequencies of Dissociation excision in Arabidopsis
20. A set of plant expression vectors for transcriptional and translational fusions
21. Strategies for mutagenesis and gene cloning using transposon tagging and T-DNA insertional mutagenesis
22. A model for RNA-mediated gene silencing in higher plants
23. A Dissociation insertion causes a semidominant mutation that increases expression of TINY, an Arabidopsis gene related to APETALA2