CKI1, a histidine kinase homolog implicated in cytokinin signal transduction

Science

Volumn 274, Issue 5289, 1996, Pages 982-985

  Kakimoto, Tatsuo ^a  

^a OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOLOGY; DNA; GENES; METABOLISM; PLANTS (BOTANY); TISSUE CULTURE;

CELL DIVISION; CYTOKININ; CYTOKININ SIGNAL TRANSDUCTION; DNA TAGGING; HISTIDINE KINASE; MUTANTS;

ENZYMES;

CYTOKININ; DNA; HISTIDINE; PHOSPHOTRANSFERASE; RNA;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; NONHUMAN; PLANT GROWTH; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; SOUTHERN BLOTTING;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CLONING, MOLECULAR; CYTOKININS; DNA, BACTERIAL; DNA, COMPLEMENTARY; DNA, PLANT; ETHYLENES; GENES, PLANT; MOLECULAR SEQUENCE DATA; MUTATION; OPEN READING FRAMES; PLANT PROTEINS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PROTEIN KINASES; RECEPTORS, CELL SURFACE; SEQUENCE HOMOLOGY, AMINO ACID; SIGNAL TRANSDUCTION; TRANSFORMATION, GENETIC;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0030575903     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5289.982     Document Type: Article

References (28)

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9. DNA isolated from the cki1-1 line was digested with Spe I, self-ligated. and used to transform Escherichia coli (DH10B) by electroporation, and the plasmid (pC1S1) was purified from the ampicillin-resistant E coli cells as described elsewhere [C. Koncz et al, in Plant Molecular Biology Manual, S. B. Gelvin and R. A. Schilperoort, Eds. (Kluwer Academic, Dordrecht, Netherlands, 1994), section B2].
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12. For construction of the Ti plasmid carrying CKl1 cDNA linked to the CaMV 35S RNA promoter, 157 bp of the 5′ noncoding sequence of cCKl1-16 was removed by treatment with exonuclease III, and all of the 3′ noncoding sequence was removed by digestion with Spe I (the stop codon is at the Spe I site). The remaining sequence, together with the 34-bp vector sequences flanking the 5′ end of the cDNA, was blunt-end cloned into the Sma I site of pMON530 [S. G. Rogers. H. J. Klee, R. B. Fraley, Methods Enzymol. 153, 253 (1987)] next to the CaMV 35S RNA promoter, and the plasmid that contained the insert in the sense orientation was selected and designated p35ScCKl1.
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27. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, lle; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
28. I thank D. Collings for correcting the English and for commenting on the manuscript, H. Shibaoka for extensive support, H. Hayashi for advice, and Y. Shinozaki for critical reading of the manuscript. Special thanks are due to R. Walden for providing plasmid pPCVICEn4HPT, for comments on the manuscript, and for continuous encouragement. pMON530 was supplied by Monsanto. Supported in part by Grants-in-Aid for Scientific Research on Priority Areas (The Molecular Basis of Flexible Organ Plans in Plants, number 06278103) and grants from the Nissan Foundation and the Sumitomo Foundation.