Targeting of PCNA to sites of DNA replication in the mammalian cell nucleus

Journal of Cellular Biochemistry

Volumn 81, Issue 1, 2001, Pages 56-67

  Diba, Fantahun ^a   Watson, Cheryl S ^b   Gametchu, Bahiru ^b  

^a MEDICAL COLLEGE OF WISCONSIN   (United States)

^b UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

Computer image analysis; Confocal microscopy; DNA replication sites in living cells; Green fluorescent protein; Normal diploid human fibroblasts

Indexed keywords

CYCLINE; DNA POLYMERASE; GREEN FLUORESCENT PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE;

ANIMAL CELL; ARTICLE; CELL CYCLE S PHASE; CELL NUCLEUS; DIPLOIDY; DNA REPLICATION; DNA SYNTHESIS; FIBROBLAST; IMAGE ANALYSIS; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN LOCALIZATION;

MAMMALIA;

EID: 0035089657     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-4644(20010401)81:1<56::aid-jcb1023>3.0.co;2-%23     Document Type: Article

References (54)

1. Growth regulation of the PCNA gene
2. The nuclear matrix: A structural milieu for nuclear genomic function
3. The new paradigm: Integrating genomic function and nuclear architecture
4. Synthesis of the nuclear protein cyclin (PCNA) and its relationship with DNA replication
5. A search for differential polypeptide synthesis throughout the cell cycle of Hela cells
6. Identification of a nuclear polypeptide ("cyclin") whose relative proportion is sensitive to changes in the rate of cell proliferation and to transformation
7. Cyclin/PCNA is the auxillary protein of DNA polymerase-δ
8. Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: Association with DNA replication sites
9. Reconstitution of human replication factor C from its five subunits in baculovirus-infected insect cells
10. Mapping and use of a sequence that directs DNA ligase I to sites of DNA replication in vivo
11. The organization of replication and transcription
12. Mcm2, but not RPA is a component of the mammalian early G1-phase prereplication complex
13. Initiation of DNA replication in eukaryotic cells
14. Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts
15. Tight control of gene expression in mammalian cells by tetracyclin-responsive promotors
16. Transcriptional activation by tetracyclines in mammalian cells
17. Intranuclear co-location of newly replicated DNA and PCNA by simultaneous immunofluorescent labelling and confocal microscopy in MCF-7 cells
18. Replication clusters are stable units of chromosome structure: Evidence that nuclear organization contributes to the efficient activation and propagation of S-phase in human cells
19. Inhibition of cellular proliferation by antisense oligodeoxynucleotides to PCNA cyclin
20. The timing of the formation and usage of replicase clusters in S-phase nuclei of human diploid fibroblasts
21. Studies on the activator 1 protein complex, an accessory factor for proliferating cell nuclear antigen-dependent DNA polymerase delta
22. Targeting and association of proteins with functional domains in the nucleus: The insoluble solution
23. A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei
24. Dynamics of DNA replication factories in living cells
25. Spatial and temporal dynamics of DNA replication sites in mammalian cells
26. S-phase patterns of cyclin (PCNA) antigen staining resemble topographical patterns of DNA synthesis. A role for cyclin in DNA replication?
27. Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labeling of DNA and confocal microscopy
28. Dynamic behavior of DNA replication domains
29. Identity of the proliferating cell nuclear antigen and cyclin
30. High-resolution detection of newly synthesized DNA by anti-bromodeoxyuridine antibodies identifies specific chromatin domains
31. An autoantibody to a nuclear antigen in proliferating cells
32. Structural organization of replicon domains during DNA synthetic phase in the mammalian nucleus
33. Mapping replicational sites in the eukaryotic cell nucleus
34. The architectural organization of nuclear metabolism
35. The cell-cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro
36. Coordinated leading and lagging stand synthesis during SV40 DNA replication in vitro requires PCNA
37. Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein
38. Proliferating cell nuclear antigen bound to DNA synthesis sites: Phosphorylation and association with cyclin D1 and cyclin A
39. Image analysis techniques for visualizing the spatial organization of DNA replication sites in the mammalian cell nucleus using multi-channel confocal microscopy
40. Macromolecular domains within the cell nucleus
41. Contributions of nuclear architecture to transcriptional control
42. Transcriptional control within the three-dimensional context of nuclear architecture: Requirements for boundaries and direction
43. An auxillary protein for DNA polymerase-d from foetal calf thymus
44. Functions of replication factor C and proliferating-cell nuclear antigen: Functional similarity of DNA polymerase accessory proteins from human cells and bacteriophage T4
45. Replication factors required for SV40 DNA replication in vitro. I. DNA structure-specific recognition of a primer-template junction by eukaryotic DNA polymerases and their accessory proteins
46. Mcm proteins in DNA replication
47. Subdivision of S-phase by analysis of nuclear 5-bromodeoxyuridine staining patterns
48. Nuclear domains and the nuclear matrix
49. The DNA replication fork in eukaryotic cells
50. Segregation of transcription and replication sites into higher order domains
51. Three-dimensional visualization of transcription sites and their association with splicing factor-rich nuclear speckles
52. Intranuclear targeting of AML/CBFalpha regulatory factors to nuclear matrixassociated transcriptional domains
53. Identification of a nuclear matrix targeting signal in the leukemia and bone-related AML/ CBF-alpha transcription factors
54. Involvement of proliferating cell nuclear antigen (cyclin) in DNA replication in living cells