Isolation of the escherichia coli nucleoid

Biochimie

Volumn 83, Issue 2, 2001, Pages 149-154

  Cunha, Sónia ^a,b   Odijk, Theo ^b   Süleymanoglu, Erhan ^a   Woldringh, Conrad L ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

Cell lysis; DNA compaction; Escherichia coli; Nucleoid isolation; Osmotic shock

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; BACTERIAL DNA; BACTERIAL RNA; DETERGENT; EDETIC ACID; LYSOZYME; RIBONUCLEASE; SODIUM CHLORIDE; SPERMIDINE;

ARTICLE; BACTERIAL CHROMOSOME; ESCHERICHIA COLI; ISOLATION PROCEDURE; LYSIS; MEMBRANE; NONHUMAN; OSMOTIC STRESS; SPHEROPLAST;

BACTERIOLYSIS; CELL FRACTIONATION; CELL NUCLEUS; CHROMOSOMES, BACTERIAL; DETERGENTS; DNA, BACTERIAL; ESCHERICHIA COLI; OSMOTIC PRESSURE; RNA, BACTERIAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035084721     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(01)01245-7     Document Type: Review

References (33)

1. Nuclear division as observed in live bacteria by a new technique
2. The chromatin bodies of bacteria
3. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states
4. Etude au microscope électronique de plasmas contenantde l'acide déoxyribonucléique. I. Les nucléoides des bactéries en croissance active
5. Visualization of bacterial genes in action
6. Characterization, assay, and use of isolated bacterial nucleoids
7. Structure of the isolated nucleoid
8. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex
9. On the structure of the folded chromosome of E. Coli
10. Replication of the Escherichia coli chromosome with a soluble enzyme system
11. Structure of DNA within the bacterial cell: Physics and physiology
12. Macromolecular crowding and the mandatory condensation of DNA in bacteria
13. Osmotic compaction of supercoiled DNA into a bacterial nucleoid
14. Folding of prokaryotic DNA: Isolation of characterization of nucleoids from Bacillus licheniformis
15. Isolation and characterization of spermidine nucleoids from Escherichia coli
16. The effect of lysozyme on DNA-membrane association in Escherichia coli
17. The bacterial nucleoid visualized by fluorescence microscopy of cells lysed within agarose: Comparison of Escherichia coli and spirochetes of the genus Borrelia
18. Effects of treatment with sodium dodecyl sulfate on the ultrastructure of Escherichia coli
19. Stabilization of compact spermidine nucleoids from Escherichia coli under crowded conditions: Implications for in vivo nucleoid structure
20. Factors affecting the release of folded chromosomes from Escherichia coli
21. Nucleoid release from Escherichia coli cells
22. Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli
23. RNA molecules bound to the folded bacterial genome stabilize DNA folds and segregate domains of supercoiling
24. Multiple restraints to the unfolding of spermidine nucleoids from Escherichia coli
25. The bacterial nucleoid
26. Co-translational insertion of envelope proteins: Theoretical considerations and implications
27. Structure and partitioning of bacterial DNA: Determined by a balance of compaction and expansion forces?
28. Hypothesis: Chromosome separation in Escherichia coli involves autocatalytic gene expression, translation and membrane-domain formation
29. Electron microscopy of chloramphenicol-treated Escherichia coli
30. Dynamics of the expanding DNA nucleoid released from a bacterial cell
31. Mutual suppression of mukB and seqA phenotypes might arise from their opposing influences on the Escherichia coli nucleoid structure
32. A dilatancy assay for nucleoid denaturation: The centrifugation-dependent clumping of denatured spermidine nucleoids
33. Image cytometric method for quantifying the relative amount of DNA in bacterial nucleoids using Escherichia coli