Substitutions in bacteriophage T4 AsiA and Escherichia coli σ70 that suppress T4 MotA activation mutations

Journal of Bacteriology

Volumn 183, Issue 7, 2001, Pages 2289-2297

  Cicero, M P ^a   Sharp, M M ^a   Gross, C A ^a   Kreuzer, K N ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; HOLOENZYME; MUTANT PROTEIN; RNA POLYMERASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR ASIA; TRANSCRIPTION FACTOR MOTA; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIOPHAGE T4; BACTERIUM MUTANT; CELL STRUCTURE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE ACTIVATION; GENE DISRUPTION; GENE MUTATION; GENETIC SELECTION; GENETIC TRANSCRIPTION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN PURIFICATION;

BACTERIOPHAGE T4; DNA-BINDING PROTEINS; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MUTATION; PROMOTER REGIONS (GENETICS); SIGMA FACTOR; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; T4 BACTERIOPHAGE; UNIDENTIFIED BACTERIOPHAGE;

EID: 0035083898     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.7.2289-2297.2001     Document Type: Article

References (39)

1. 70 subunit is responsible for the recognition of the 'extended -10' motif at promoters
2. Role of MotA transcription factor in bacteriophage T4 DNA replication
3. Extended - 10 promoters
4. Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins
5. The bacteriophage T4 AsiA protein: A molecular switch for sigma 70-dependent promoters
6. 70 exhibit specificity of binding to promoter DNA
7. Induced mutagenesis and isolation of T4 mutants
8. The DNA binding domain of the MotA transcription factor from bacteriophage T4 shows structural similarity to the TATA-binding protein
9. MotA transcription factor from bacteriophage T4 has an acidic activation domain
10. An N-terminal mutation in the bacteriophage T4 motA gene yields a protein that binds DNA but is defective for activation of transcription
11. The functional and regulatory roles of sigma factors in transcription
12. Transcription from bacteriophage T4 middle promoter using T4 MotA protein and phage-modified RNA polymerase
13. The bacteriophage T4 middle transcription system: T4-modified RNA polymerase, Asia (sigma-70 binding protein), and the transcriptional activator MotA
14. Mutations in rpoD that increase expression of genes in the mal regulon of Escherichia coli K-12
15. Protein-protein communication within the transcription apparatus
16. 70 subunit of Escherichia coli RNA polymerase in transcriptional activation by activator protein PhoB
17. A defective phage system reveals bacteriophage T4 replication origins that coincide with recombination hot spots
18. Characterization of a defective phage system for the analysis of bacteriophage T4 replication origins
19. 70 that result in defects in phage Λ repressor-stimulated transcription
20. 70 subunit of Escherichia coli RNA polymerase in transcription activation
21. Target of transcriptional activation function of phage Λ cI protein
22. 70 subunit
23. 70 subunit of RNA polymerase in transcriptional activation by activator protein PhoB in Escherichia coli
24. pACYC184-derived cloning vectors containing the multiple cloning site and lacZα reporter gene of pUC8/9 and pUC18/19 plasmids
25. Deletion analysis of bacteriophage T4 tertiary origins
26. 70 that are involved in interaction with RNA polymerase core enzyme
27. Bacteriophage T4 MotA and AsiA proteins suffice to direct Escherichia coli RNA polymerase to initiate transcription at T4 middle promoters
28. Host-virus interactions in the control of T4 prereplicative transcription. II. Interaction between tabC(rho) mutants and T4 mot mutants
29. Purified MotA protein binds the -30 region of a bacteriophage T4 middle promoter and activates transcription in vitro
30. The bacteriophage T4 insertion/substitution vector system
31. Expressed protein ligation, a novel method for studying protein-protein interactions in transcription
32. Inhibition of Escherichia coli RNA polymerase by bacteriophage T4 AsiA
33. 70 subunit
34. 70 fragment
35. The interface of σ with core RNA polymerase is extensive, conserved, and functionally specialized
36. Regulation of middle-mode transcription
37. Effects of umuDC, mucAB, and samAB operons on the mutational specificity of chemical mutagenesis in Escherichia coli: II. Base substitution mutagenesis
38. Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase