Study of the interaction between bacteriophage T4 and asiA and Escherichia coli σ70, using the yeast two-hybrid system: Neutralization of asiA toxicity to E. coli cells by coexpression of a truncated σ70 fragment

Journal of Bacteriology

Volumn 181, Issue 18, 1999, Pages 5855-5859

  Sharma, Umender K ^a   Ravishankar, Sudha ^a   Shandil, Radha Krishan ^a   Praveen, P V K ^a   Balganesh, T S ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BETA GALACTOSIDASE; PROTEIN; SIGMA FACTOR;

AMINO ACID SEQUENCE; ARTICLE; BACTERIOPHAGE T4; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; HYBRID; NONHUMAN; PRIORITY JOURNAL; TRANSCRIPTION REGULATION; YEAST;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; T4 BACTERIOPHAGE; T4 PHAGE; UNIDENTIFIED BACTERIOPHAGE;

EID: 0032834651     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.18.5855-5859.1999     Document Type: Article

References (24)

1. 70 subunit of E. coli RNA polymerase. J. Biol. Chem. 272:27435-27443.
2. Borukhow, S., and A. Goldfarb. 1993. Recombinant E. coli RNA polymerase: purification of individually overexpressed subunits and in-vitro assembly. Protein Expr. Purif. 4:503-511.
3. Brody, E. N., G. A. Cassavetis, M. Ouhammouch, G. M. Sanders, and E. P. Geiduschek. 1995. Old phage, new insights: two recently recognised mechanisms of transcriptional regulation in bacteriophage T4 development. FEMS Microbiol. Lett. 128:1-8.
4. Brown, K. L., and K. T. Hughes. 1995. The role of anti-sigma factors in gene regulation. Mol. Microbiol. 16:397-404.
5. Chien, C., P. L. Bartel, R. Sternglanz, and S. Fields. 1991. The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA 88:9578-9582.
6. Colland, F., G. Orsini, E. N. Brody, H. Buc, and A. Kolb. 1998. The bacteriophage T4 asiA: a molecular switch for sigma 70 dependent promoters. Mol. Microbiol. 27:819-829.
7. Das, A., L. B. Anderson, and Y.-H. Xie. 1997. Delineation of interaction domains of Agrobacterium tumefaciens VirB7 and VirB9 by use of the yeast two-hybrid assay. J. Bacteriol. 179:3404-3409.
8. Dombroski, A. J., W. A. Walter, and C. A. Gross. 1993. Amino terminal amino acids modulate sigma factor DNA binding activity. Genes Dev. 7: 2446-2455.
9. 70 exhibit specificity of binding to DNA. Cell 70:501-512.
10. Estojak, J., R. Brent, and E. A. Golemis. 1995. Correlation of two-hybrid affinity data with in vitro measurements. Mol. Cell. Biol. 15:5820-5829.
11. Fields, S., and R. Sternglanz. 1994. The two hybrid system: an assay for protein-protein interactions. Trends Genet. 10:286-292.
12. Fields, S., and O. Song. 1989. A novel genetic system to detect protein-protein interactions. Nature 340:245-246.
13. Golemis, E., J. Gyuris, and R. Brent. 1994. Interaction trap/two-hybrid system to identify interacting proteins, p. 13.14.1-13.14.17. In F. M. Ausubel et al. (ed.), Current protocols in molecular biology. John Wiley and Sons, Inc., New York, N.Y.
14. 70 binding protein; and transcriptional activator MotA. Methods Enzymol. 274:43-57.
15. Miller, J. 1992. A short course in bacterial genetics: a laboratory manual and handbook for E. coli and related bacteria. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
16. Orsini, G., M. Ouhammouch, J. P. Le Caer, and E. N. Brody. 1993. The asiA bacteriophage T4 codes for the anti-sigma 70 protein. J. Bacteriol. 175:85-93.
17. Ouhammouch, M., G. Orsini, and E. N. Brody. 1994. The asiA gene product of bacteriophage T4 is required for middle mode RNA synthesis. J. Bacteriol. 176:3956-3965.
18. Ouhammouch, M., K. Adelman, S. R. Harvey, G. Orsini, and E. N. Brody. 1995. Bacteriophage T4 motA and asiA proteins suffice to direct E. coli RNA polymerase to initiate transcription at T4 middle promoters. Proc. Natl. Acad. Sci. USA 92:1451-1455.
19. Severinova, E., K. Severinov, and S. A. Darst. 1998. Inhibition of Escherichia coli RNA polymerase by T4 AsiA. J. Mol. Biol. 279:9-18.
20. 70 subunit. J. Mol. Biol. 262:637-647.
21. Sharma, U. K., M. Balganesh, V. V. Vaidyanathan, R. K. Shandil, P. Kaur, and T. S. Balganesh. Unpublished data.
22. Stevens, A. 1976. A salt promoted inhibitor of RNA polymerase isolated from T4 phage-infected E. coli, p. 617-627. In R. Losick and M. Chamberlin (ed.), RNA polymerase. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
23. B regulators. J. Bacteriol. 178:7020-7023.
24. White, M. A. 1996. The yeast two-hybrid system: forward and reverse. Proc. Natl. Acad. Sci. USA 93:10001-10003.