Molecular heterogeneity of O-acetylserine sulfhydrylase by two-photon excited fluorescence fluctuation spectroscopy

Biophysical Journal

Volumn 80, Issue 4, 2001, Pages 1973-1985

  Chirico, Giuseppe ^a   Bettati, Stefano ^b   Mozzarelli, Andrea ^b   Chen, Yan ^c   Müller, Joachim D ^c   Gratton, Enrico ^c  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b UNIVERSITY OF PARMA   (Italy)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE SYNTHASE;

ARTICLE; ENZYME MECHANISM; FLUORESCENCE SPECTROSCOPY; NONHUMAN; PHOTOCHEMISTRY; SALMONELLA TYPHIMURIUM;

BACTERIA (MICROORGANISMS); SALMONELLA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0035070985     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)76167-X     Document Type: Article

References (10)

1. Aragon, S. R., and R. Pecora. 1976. Fluorescence correlation spectroscopy as a probe of molecular dynamics. J. Chem. Phys. 64:1791-1803.
2. Arrio-Dupont, M. 1970. Etude par fluorescence de bases de Schiff du pyridoxal, comparaison avec la L-aspartate-aminotransferase. Photochem. Photobiol. 12:297-315.
3. Becker, M. A., N. M. Kredich, and G. M. Tomkins. 1969. Purification and characterization of cysteine synthetase, a bifunctional protein complex, from Salmonella typhimurium. J. Biol. Chem. 244:2418-2427.
4. Beechem, J. M., and E. Gratton. 1988. Fluorescence spectroscopy data analysis environment: a second generation global analysis program. In Time Resolved Laser Spectroscopy in Biochemistry, J. R. Larowicz, ed. SPIE Proc. 909:70-81.
5. Benci, S., S. Vaccari, A. Mozzarelli, and P. F. Cook. 1999. Time-resolved fluorescence of O-acetylserine sulfhydrylase. Biochim. Biophys. Acta. 1429:317-330.
6. Berland, K. M., P. T. So, Y. Chen, W. W. Mantulin, and E. Gratton. 1996. Scanning two-photon fluctuation correlation spectroscopy: particle counting measurements for detection of molecular aggregation. Biophys. J. 71:410-420.
7. Bieschke, J., A. Giese, W. Schulz-Schaeffer, I. Zerr, S. Poser, M. Eigen, and H. Kretzschmar. 2000. Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets. Proc. Natl. Acad. Sci. U.S.A. 97:5468-5473.
8. Burkhard, P., G. S. J. Rao, E. Hohenester, K. D. Schnackerz, P. F. Cook, and J. N. Jansonius. 1998. Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium. J. Mol. Biol. 282:121-133.
9. Chandrasekhar, S. 1943. Stochastic problems in physics and astronomy. Rev. Mod. Phys. 15:1-89.
10. Chen, Y., J. D. Muller, P. T. C. So, and E. Gratton. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys. J. 77:553-567.