Selective culture of epithelial cells from primary breast carcinomas using irradiated 3T3 cells as feeder layer

Pathology Research and Practice

Volumn 197, Issue 3, 2001, Pages 175-181

  Wang, C S ^a   Goulet, F ^b   Tremblay, N ^b   Germain, L ^b   Auger, F ^b   Tetu B ^a  

^a LAVAL UNIVERSITY   (Canada)

^b Centre Hospitalier Affilié   (Canada)

Author keywords

Tumor associated fibroblasts; Tumor derived epithelial cells

Indexed keywords

CYTOKERATIN; ENZYME; EPITHELIAL MEMBRANE ANTIGEN;

ADULT; AGED; ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; BREAST CARCINOMA; BREAST EPITHELIUM; CELL ADHESION; CELL CULTURE; CELL GROWTH; CELL STRAIN 3T3; CELL STRUCTURE; CLINICAL ARTICLE; COCULTURE; CONTROLLED STUDY; EPITHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; HUMAN TISSUE; METHODOLOGY; NONHUMAN; PROTEIN EXPRESSION; PURIFICATION; RADIATION EXPOSURE;

EID: 0035068924     PISSN: 03440338     EISSN: None     Source Type: Journal    
DOI: 10.1078/0344-0338-00030     Document Type: Article

References (23)

1. Establishment and characterization of a new cell line from primary human breast carcinoma
2. Cocultivation of human primary breast carcinomas and embryonic mesenchyme resulting in growth and maintenance of tumor cells
3. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts
4. Fibroblast-mediated acceleration of human epithelial tumor growth in vivo
5. Assessment of methods for primary tissue culture of human breast epithelia
6. Selective cell culture of primary breast carcinoma
7. Partial enzymatic degradation of stroma allows enrichment and expansion of primary breast tumor cells
8. Vimentin is preferentially expressed in human breast carcinomas with low estrogen receptor and high Ki-67 growth fraction
9. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines
10. Differential isolation of normal luminal mammary epithelial cells and breast cancer cells from primary and metastatic sites using selective media
11. Improvement of human keratinocyte isolation and culture using thermolysin
12. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting
13. Growth of normal and malignant human mammary epithelial cells in culture
14. Clonal expansion of epithelial cells from primary human breast carcinoma with 3T3 feeder layer technique
15. Long-term culture of primary breast cancer in defined medium
16. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells
17. Clonal characterization of mouse mammary luminal epithelial and myoepithelial cells separated by fluorescence-activated cell sorting
18. Clonal proliferation of cultured nonmalignant and malignant human breast epithelia
19. Short-term primary culture of epithelial cells derived from human breast tumours
20. Effects of human bone marrow stroma on the growth of human tumor cells
21. Separation of tumor cells from fibroblasts with use of discontinuous density gradients
22. Growth requirements of human mammary epithelial cells in culture
23. Establishment and characterization of a new cell line derived from human primary breast carcinoma