Digital imaging microscopy of firefly luciferase activity to directly monitor differences in cell transduction efficiencies between AdCMVLuc and Ad5LucRGD vectors having different cell binding properties

Journal of Virological Methods

Volumn 93, Issue 1-2, 2001, Pages 175-179

  Kratzer, Sylvia ^a   Mundigl, Olaf ^a   Dicker, Frank ^a   Seeber, Stefan ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

Adenoviral vector; Adenovirus; Digital imaging microscopy; Luciferase; Reporter gene

Indexed keywords

LUCIFERASE; LUCIFERIN; TISSUE EXTRACT; VIRUS VECTOR;

ADENOVIRUS; ARTICLE; CHEMOLUMINESCENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; FIBROBLAST; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; MICROSCOPY; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE;

ADENOVIRIDAE; CELL LINE; CHEMILUMINESCENT MEASUREMENTS; FIREFLY LUCIFERIN; GENES, REPORTER; GENETIC VECTORS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LUCIFERASES; MICROSCOPY, PHASE-CONTRAST; RECEPTORS, IMMUNOLOGIC; RECEPTORS, PEPTIDE; TRANSFECTION;

ADENOVIRIDAE;

EID: 0035054046     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(01)00259-2     Document Type: Article

References (8)

1. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5
2. Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells
3. An Adenovirus vector with genetically modified fibers demonstrates expanded tropism via utilization of a Coxsackievirus and Adenovirus receptor-independent cell entry mechanism
4. Video imaging of firefly luciferase activity to identify and monitor herpesvirus infection in cell culture
5. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy
6. Insertion of an RGD motif into the HI loop of adenovirus fiber protein alters the distribution of transgene expression of the systemically administered vector
7. An advanced generation of adenoviral vectors selectively enhances gene transfer for ovarian cancer gene therapy approaches
8. 5 promote adenovirus internalisation but not virus attachment