Effect of ions and nucleotides on the interactions of yeast Rad51 protein with single-stranded oligonucleotides

Journal of Biochemistry

Volumn 129, Issue 3, 2001, Pages 469-475

  Kim, Jong Moon ^a   Maraboeuf, Fabrice ^b   Kim, Seog K ^a   Shinohara, Akira ^c   Takahashi, Masayuki ^b  

^a Yeungnam University   (South Korea)

^b UNIVERSITÉ DE NANTES   (France)

^c OSAKA UNIVERSITY   (Japan)

Author keywords

DNA binding; Homologous recombination; Nucleotide cofactor; Rad51 protein; RecA protein

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ION; MAGNESIUM ION; NUCLEOTIDE; OLIGONUCLEOTIDE; RAD51 PROTEIN; RECA PROTEIN; SINGLE STRANDED DNA;

ARTICLE; ESCHERICHIA COLI; EUKARYOTE; GENETIC RECOMBINATION; HYDROLYSIS; NONHUMAN; PROTEIN DNA INTERACTION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; YEAST;

EARIAS; ESCHERICHIA COLI; EUKARYOTA; XENOPUS;

EID: 0035053510     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a002879     Document Type: Article

References (43)

1. Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the Rad51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins
2. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein
3. Cloning of human, mouse and fission yeast genes homologous Rad51 and recA
4. Evolutionary comparisons of RecA-like proteins acrossall major kingdoms of living organisms
5. Homologous recombinationand the roles of double-strand breaks
6. Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein
7. Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro
8. Purificationand characterization of XRad51.1 protein. Xenopus Rad51 homolog: Recombinant XRad51.1 promotes strand exchange reaction
9. Activities of human recombination protein Rad51
10. Biochemistry of homologousrecombination in Escherichia coli
11. Structure of RecA-DNA complex and mechanism of DNA strand exchange reaction in homologous recombination
12. RecA protein: Structure, function, and role in recombination DNA repair
13. Similarity of the yeast RAD51 filament to the bacterial RecA filament
14. Purification and characterization of the human Rad51 protein, an analog of E. coli RecA
15. Nucleotide cofactor dependent structural change of Xenopus Rad51 protein filament detected by small-angle neutron scattering measurements in solution
16. Stimulation by Rad52 of yeast Rad51-mediated recombination
17. Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A
18. Binding of Rad51p to DNA. Interaction of Rad51p with single- and double-stranded DNA
19. Nucleotide dependent structural and kinetic changes in Xenopus Rad51.1-DNA complex stimulating the strand exchange reaction: Destacking of DNA bases and restriction of their local motion
20. The DNA binding properties of Saccharomyces cerevisae Rad51 protein
21. The hRad51 and RecA proteins show significant differences in cooperative binding to single-stranded DNA
22. Purified Escherichia coli RecA protein catalyzes homologous pairing of superhelical DNA and single-stranded fragments
23. Fibers of RecA protein and complexes of RecA protein and single-stranded φX174 DNA as visualized by negative-stain electron microscopy
24. Binding stoichiometry and structure of RecA-DNA complexes studied by the linear dichroism and fluorescence spectroscopy, evidence for multiple heterogeneous DNA coordination
25. Cofacter induced orientation of the DNA bases in single-stranded DNA complexed with RecA protein: A fluorescence anisotropy and time-decay study
26. Interaction of recA protein with single-stranded DNA. Quantitative aspect of binding affinity modulation by nudeotide cofactors
27. A simple and rapid procedures for the large scale purification of the recA protein of Escherichia coli
28. Kinetic studies of recA protein binding to a fluorescent single-stranded polynucleotide
29. Properties of the duplex DNA-dependent ATPase activity of Escherichia coli RecA protein and its role in branch migration
30. Thermochemical and kinetic evidence for necleotide-sequence-defendant RecA-DNA interactions
31. The physical and enzymatic properties of Escherichia coli recA protein display anion-specific inhibition
32. - recombination by the mutagenesis complex UmuD'C
33. Use of fluorescent DNA analog for fluorometric detection of DNase activity
34. Thermodynamic analysis of ion effects on the binding and conformational equilibria of proteins and nucleic acids: The roles of ion association or release, screening, and ion effects on water activity
35. Analysis of DNA-RecA protein interactions involving the protein self-association reaction
36. Interaction of Escherichia coli RecA protein with ATP and its analogues
37. Properties of the high-affinity single-stranded DNA binding state of the Escherichia coli RecA protein
38. Use of fiuorescein-labeled oligonucleotide for analysis of formation and dissociation kinetics of T:A:T triple-stranded DNA: Effect of divalent cations
39. Difference between active and inactive nucleotide cofactor of RecA in the effect on the DNA binding and the structure of RecA filament: Dissociation of RecA/DNA complex by inactive nucleotides
40. Biochemistry of genetic recombination: Energetics and mechanism of DNA strand exchange
41. Rapid exchange of A:T base pair isessential for recognition of DNA homology by human Rad51 recombination protein
42. Yeast Rad51 recombinase mediates polar DNA strand exchange in the absence of ATP hydrolysis