Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step

Journal of Virology

Volumn 75, Issue 9, 2001, Pages 4376-4385

  Bryant, H E ^a   Wadd, S E ^a   Lamond, A I ^a   Silverstein, S J ^a   Clements, J B ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT; PROTEIN IE63; SPLICEOSOME ASSOCIATED PROTEIN 145; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CATALYSIS; COMPLEX FORMATION; CONTROLLED STUDY; HERPES SIMPLEX VIRUS; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RNA PROCESSING; SEQUENCE HOMOLOGY; SPLICEOSOME; VIRUS INFECTION; VIRUS MUTANT; VIRUS TRANSCRIPTION;

BINDING SITES; CATALYSIS; HELA CELLS; HERPESVIRUS 1, HUMAN; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEINS; HUMANS; IMMEDIATE-EARLY PROTEINS; RIBONUCLEOPROTEINS; RNA SPLICING; RNA-BINDING PROTEINS; SPLICEOSOMES;

EID: 0035050518     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.9.4376-4385.2001     Document Type: Article

References (69)

1. Characterization of herpes simplex virus 1 α proteins 0, 4, and 27 with monoclonal antibodies
2. L15, a highly conserved herpes simplex virus 1 gene, effectively replaces the two exons of the wild-type virus
3. Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions
4. Interaction between herpes simplex virus type 1 IE63 protein and cellular protein p32
5. Identification of hnRNP P2 as TLS/FUS using electrospray mass spectrometry
6. Alternatively spliced mRNAs predicted to yield frame-shift proteins and stable intron 1 RNAs of the herpes simplex virus regulatory gene α0 accumulate in the cytoplasm of infected cells
7. The prespliceosome components SAP49 and SAP145 interact in a complex implicated in tethering U2 snRNP to the branch site
8. Herpes simplex virus ICP27 induces cytoplasmic accumulation of unspliced polyadenylated α-globin pre-mRNA in infected HeLa cells
9. An unusual spliced herpes simplex virus type 1 transcript with sequence homology to Epstein-Barr virus DNA
10. Characterisation of the nucleic acid binding activity of KH domains. Different properties of different domains
11. Isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein ICP4
12. Processing of α-globin in cells infected with herpes simplex virus type 1 ICP27 mutants
13. Trans-activation of transcription by herpes virus products: Requirement for two HSV-1 immediate-early polypeptides for maximum activity
14. A truncated form of herpes-simplex virus type 1 immediate-early protein Vmw110 is expressed in a cell-type dependent manner
15. Identification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene
16. The regions important for the activator and repressor functions of herpes simplex virus type 1 α protein ICP27 map to the C-terminal half of the molecule
17. The herpes simplex virus regulatory protein ICP27 contributes to the decrease in cellular mRNA levels during infection
18. Herpes simplex virus inhibits host cell splicing, and regulatory protein ICP27 is required for this effect
19. SnRNP Sm proteins share two evolutionarily conserved motifs which are involved in Sm protein-protein interactions
20. Herpes simplex virus turns off the TAP to evade host immunity
21. Immediate early protein IE63 of herpes simplex virus type 1 binds RNA directly
22. Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs
23. Interactions between small nuclear ribonucleoprotein particles in formation of spliceosomes
24. Structure and function in the nucleus
25. A mutational analysis of spliceosome assembly: Evidence for splice site collaboration during spliceosome formation
26. Probing the structure and function of U2 Sn RNP with antisense oligonucleotides made of 2′-Ome RNA
27. Early commitment of yeast pre-mRNA to the spliceosome pathway
28. Redistribution of the nuclear ribonucleoprotein antigens during herpes simplex virus infection
29. Herpes simplex virus type 1 ICP27 deletion mutants exhibit altered patterns of transcription and are DNA deficient
30. Herpes simplex virus
31. Regulation of herpes simplex virus type 1 poly(A) site usage and the action of the immediate-early protein IE63 in the early-late switch
32. The repressing and enhancing functions of herpes simplex virus regulatory protein ICP27 map to the C-terminal regions and are required to modulate viral gene expression very early in infection
33. Identification of nuclear and nucleolar localization signals in the herpes simplex virus regulatory protein ICP27
34. The RGG box motif of the herpes simplex virus ICP27 protein mediates an RNA-binding activity and determines in vivo methylation
35. The herpes simplex virus immediate-early protein ICP27 shuttles between nucleus and cytoplasm
36. Heterogeneous nuclear ribonucleoprotein K is a transcription factor
37. Evidence for a direct role for both the 175,000- and 110,000-molecular-weight immediate-early proteins of herpes simplex virus in the transactivation of delayed-early promoters
38. Lariat RNAs as intermediates and products in the splicing of messenger RNA precursors
39. A herpes simplex virus type 1 immediate-early gene product, IE63, regulates small nuclear ribonucleoprotein distribution
40. Herpes simplex virus type 1 immediate-early protein IE63 shuttles between nuclear compartments and the cytoplasm
41. Posttranscriptional regulation in herpes simplex virus
42. The HIV Rev protein
43. Mechanisms of fidelity in pre-mRNA splicing
44. Gene-specific transactivation by herpes simplex virus type 1 α protein ICP27
45. Genetic evidence for two distinct transactivation functions of the herpes simplex virus α protein ICP27
46. The acidic amino-terminal region of herpes simplex virus type 1 alpha protein ICP27 is required for an essential lytic function
47. Amino acid substitution mutations in the herpes simplex virus ICP27 protein define an essential gene regulation function
48. Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro
49. Herpes simplex virus type 1 ICP27 is an essential regulatory protein
50. Deletion mutants in the gene encoding the herpes simplex virus type 1 immediate-early protein ICP0 exhibit impaired growth in cell culture
51. ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif
52. The C-terminal repressor region of herpes simplex virus type 1 ICP27 is required for the redistribution of small nuclear ribonucleoprotein particles and splicing factor SC35; however, these alterations are not sufficient to inhibit host cell splicing
53. Herpes simplex virus 1 mutant deleted in the α22 gene: Growth and gene expression in permissive and restrictive cells and establishment of latency in mice
54. Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells
55. The herpes simplex virus type 1 α protein ICP27 can act as a trans-repressor or a trans-activator in combination with ICP4 and ICP0
56. Essential role for KH domains in RNA binding: Impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome
57. Evidence that the herpes simplex virus immediate-early protein ICP27 acts post-transcriptionally during infection to regulate gene expression
58. Shuttling of the herpes simplex virus type I regulatory protein ICP27 between the nucleus and the cytoplasm mediates the expression of late proteins
59. Herpesvirus mRNAs are sorted for export via Crm-1-dependent and -independent pathways
60. Identification of an export control sequence and a requirement for the KH domains in ICP27 from herpes simplex virus type 1
61. Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly
62. Herpes simplex virus α protein ICP27 can inhibit or augment viral gene transactivation
63. Yeast Sm-like proteins function in mRNA decapping and decay
64. Herpes simplex ICP27 mutant viruses exhibit reduced expression of specific DNA replication genes
65. The herpes simplex virus immediate early protein ICP27 encodes a potential metal binding domain and binds zinc in vitro
66. The multifunctional herpes simplex virus IE63 protein interacts with heterogeneous ribonucleoprotein K and with casein kinase 2
67. A herpes simplex virus type 1 function required for early and late virus RNA synthesis
68. Self-interaction of the herpes simplex virus type 1 regulatory protein ICP 27