The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site

Genes and Development

Volumn 8, Issue 16, 1994, Pages 1974-1983

  Champion Arnaud, Patrick ^a   Reed, Robin ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

branchpoint sequence; prespliceosome; proline domain; protein protein interaction; U2 snRNP

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA PRECURSOR; NUCLEAR PROTEIN; SMALL NUCLEAR RIBONUCLEOPROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; CROSS LINKING; HELA CELL; HUMAN; HUMAN CELL; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RNA STRUCTURE; SPLICEOSOME;

MAMMALIA;

EID: 0028144831     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.8.16.1974     Document Type: Article

References (16)

1. Amrein, H., M.L. Hedley, and T. Maniatis. 1994. The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by transformer 2. Cell 76: 735-746.
2. Barabino, S.M., B.J. Blencowe, U. Ryder, B.S. Sproat, and A.I. Lamond. 1990. Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly. Cell 63: 293-302.
3. Behrens, S-E., K. Tyc, B. Kastner, J. Reichelt, and R. Luhrmann. 1993a. Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions. Mol. Cell Biol. 13: 307-319.
4. Behrens, S-E., F. Galisson, P. Legrain, and R. Luhrmann. 1993b. Evidence that the 60 kD protein of 17S U2 snRNP is immunologically and functionally related to the yeast PRP9 splicing factor and is required for efficient formation of prespliceosomes. Proc. Natl. Acad. Sci. 90: 8229-8233.
5. Bennett, M. and R. Reed. 1993. Correspondence between a mammalian spliceosome component and an essential yeast splicing factor. Science 262: 105-108.
6. Bennett, M., S. Michaud, J. Kingston, and R. Reed. 1992a. Protein components specifically associated with prespliceosome and spliceosome complexes. Genes & Dev. 6: 1986-2000.
7. Bennett, M., S. Piñol-Roma, D. Staknis, G. Dreyfuss, and R. Reed. 1992b. Transcript-dependent packaging of pre-mRNA in hnRNP complexes prior to spliceosome assembly in vitro. Mol. Cell. Biol. 12: 3165-3175.
8. Black, D., B. Chabot, and J.A. Steitz. 1985. U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing. Cell 42: 737-750.
9. Staknis, D. and R. Reed. 1994. Direct interactions between premRNA and six U2 snRNP proteins during spliceosome assembly. Mol. Cell Biol. 14: 2994-3005.
10. Wassarman, D.A. and J.A. Steitz. 1992. Interactions of small nuclear RNAs with precursor messenger RNA during in vitro splicing. Science 257: 1918-1925.
11. Wu, J.Y. and J.L. Manley. 1989. Mammalian pre-mRNA branch site selection by U2 snRNP involves base pairing. Genes & Dev. 3: 1553-1561.
12. Wyatt, J.R., E.J. Sontheimer, and J.A. Steitz. 1992. Site-specific cross-linking of mammalian U5 snRNP to the 5′ splice site prior to the first step of premessenger RNA splicing. Genes & Dev. 6: 2542-2553.
13. Zamore, P.D. and M.R. Green. 1989. Identification, purification and biochemical characterization of U2 small nuclear ribonucleoprotein auxilary factor. Proc. Natl. Acad. Sci. 86: 9243-9247.
14. Zhang, M., P.D. Zamore, M. Carmo-Fonesca, A.I. Lamond, and M.R. Green. 1992. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small unit. Proc. Natl. Acad. Sci. 89: 8769-8773.
15. Zhuang, Y. and A.M. Weiner. 1989. A compensatory base change in human U2 snRNA can suppress a branch site mutation. Genes & Dev. 3: 1545-1552.
16. Zhuang, Y., A.M. Goldstein, and A.M. Weiner. 1989. UAC-UAAC is the preferred branch site for mammalian pre-mRNA splicing. Proc. Natl. Acad. Sci. 86: 2752-2756.