Specific blocking to improve biopanning in biological samples such as serum and hybridoma supernatants

BioTechniques

Volumn 30, Issue 4, 2001, Pages 798-802

  Messmer, B T ^a   Thaler, D S ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; FLOWCHARTING;

BIOPANNING;

BIOTECHNOLOGY;

EPITOPE; IMMUNOGLOBULIN M; IMMUNOGLOBULIN M ANTIBODY; POLYCLONAL ANTIBODY; SYNTHETIC PEPTIDE;

ARTICLE; BACTERIOPHAGE LAMBDA; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; HYBRIDOMA; MASS SPECTROMETRY; NONHUMAN; PEPTIDE ANALYSIS; PHAGE DISPLAY; SERUM;

BACTERIOPHAGE LAMBDA; ESCHERICHIA COLI;

EID: 0035050136     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01304st06     Document Type: Article

References (12)

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2. Encoded combinatorial chemistry
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4. Convergent solutions to binding at a protein-protein Interface
5. Peptide ligands that bind IgM antibodies and block interaction with antigen
6. Screening of phage antibody libraries
7. A sandwich method of enzymoimmunoassay. I. Application to rat and human alpha-fetoprotein
8. Sequential determination of ligands binding to discrete components in heterogeneous mixtures by iterative panning and blocking (IPAB)
9. Two human neonatal IgM antibodies encoded by different variable-region genes bind the same linear peptide: Evidence for a stereotyped repertoire of epitope recognition
10. Searching for peptide ligands with an epitope library
11. Libraries of peptides and proteins displayed on filamentous phage
12. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase