Application of pressure steps to mechanosensitive channels in membrane patches: A simple, economical, and fast system

Pflugers Archiv European Journal of Physiology

Volumn 442, Issue 1, 2001, Pages 150-156

  Hurwitz, Craig G ^b   Segal, Alan S ^a,b  

^a University of Vermont ^*  (United States)

^b UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

Instrumentation; Ion channels; Kidney; Mechanosensitive; Patch clamp technique; Pressure; Stretch activated

Indexed keywords

CATION CHANNEL;

ARTICLE; CHANNEL GATING; MECHANORECEPTOR; MOLECULAR DYNAMICS; PATCH CLAMP; PRESSURE; PRIORITY JOURNAL;

AMBYSTOMA; ANIMALS; CELL MEMBRANE; ION CHANNEL GATING; ION CHANNELS; KIDNEY TUBULES, PROXIMAL; MECHANORECEPTORS; PATCH-CLAMP TECHNIQUES; PRESSURE;

EID: 0035049671     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240100541     Document Type: Article

References (13)

1. Mechanotransducing ion channels in C6 glioma cells
2. Stretch-activated single ion channel currents in tissue-cultured embryonic chick skeletal muscle
3. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches
4. Functional significance of cell volume regulatory mechanisms
5. Pressure-clamp: A method for rapid step perturbation of mechanosensitive channels
6. Pressure-clamp technique for measurement of the relaxation kinetics of mechanosensitive channels
7. A fast pressure-clamp technique for studying mechanogated channels
8. Simplified fast pressure-clamp technique for studying mechanically gated channels
9. Mechanosensitive ion channels in nonspecialized cells
10. A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension
11. Voltage-induced slow activation and deactivation of mechanosensitive channels in Xenopus oocytes
12. Delayed activation of single mechanosensitive channels in Lymnaea neurons
13. Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels