Genotyping method for point mutation detection in the endothelial nitric-oxide synthase exon 7 using fluorescent probes. Clinical validation in systemic sclerosis patients

Clinical Chemistry and Laboratory Medicine

Volumn 39, Issue 3, 2001, Pages 281-282

  Biondi, M L ^a   Marasini, B ^a   Leviti, S ^a   Turri, O ^a   Bernini, M ^a   Seminati, R ^a   Porreca, W ^a   Guagnellini, E ^a  

^a SAN PAOLO HOSPITAL   (Italy)

Author keywords

Fluorescent probes; NO synthase exon 7 polymorphism; Systemic sclerosis

Indexed keywords

ACRYLAMIDE; ADENINE; ASPARTIC ACID; DEOXYADENOSINE TRIPHOSPHATE; DEOXYURIDINE; DNA; EDETIC ACID; ETHIDIUM BROMIDE; FLUORESCENT DYE; GLUTAMIC ACID; NITRIC OXIDE SYNTHASE; REAGENT; RESTRICTION ENDONUCLEASE; RHODAMINE; THYMINE;

ADULT; AGED; ALLELE; ASSAY; CONTROLLED STUDY; DNA CONTENT; EXON; EXTRACTION; FEMALE; FLUORESCENCE; GENE AMPLIFICATION; GENE FREQUENCY; GENETIC POLYMORPHISM; GENETIC SUSCEPTIBILITY; GENOTYPE; HUMAN; LETTER; MAJOR CLINICAL STUDY; MISSENSE MUTATION; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAYNAUD PHENOMENON; SYSTEMIC SCLEROSIS; VALIDATION PROCESS; VASCULAR ENDOTHELIUM;

ADULT; EXONS; FEMALE; FLUORESCENT DYES; GENOTYPE; HUMANS; MALE; MIDDLE AGED; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; POINT MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; REPRODUCIBILITY OF RESULTS; SCLERODERMA, SYSTEMIC;

EID: 0035034922     PISSN: 14346621     EISSN: None     Source Type: Journal    
DOI: 10.1515/CCLM.2001.043     Document Type: Letter

References (10)

1. Structure and chromosomal localization of human constitutive endothelial nitric oxide synthase gene
2. Nitric oxide and its role in the cardiovascular system
3. Studies of the microvascular endothelium in uninvolved skin of patients with systemic sclerosis: Direct evidence for a generalized microangiopathy
4. Endothelial expression of nitric oxide synthase and nitrotyrosine in systemic sclerosis skin
5. A common variant of the nitric oxide synthase gene is a risk factor for coronary atherosclerosis in the east Anglia region of UK
6. Association of the missense Glu298Asp variant of the endothelial nitric oxide synthase gene with myocardial infarction
7. Endothelial nitric oxide synthase exon 7 polymorphism, ischaemic cerebrovascular disease, and carotid atheroma
8. Subcommittee for scleroderma criteria of the American rheumatism association diagnostic and therapeutic criteria committee: Preliminary criteria for the classification of systemic sclerosis (scleroderma)
9. Allelic discrimination using fluorogenic probes and the 5′nuclease assay
10. Genotyping method for point mutation detection in the intestinal fatty acid binding protein, using fluorescent probes