The C-terminal tetrapeptide of phaseolin is sufficient to target green fluorescent protein to the vacuole

Journal of Plant Physiology

Volumn 158, Issue 4, 2001, Pages 499-503

  Frigerio, Lorenzo ^a   Foresti, Ombretta ^b   Felipe, Doramys Hernández ^c   Neuhaus, Jean Marc ^c   Vitale, Alessandro ^b  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b CNR   (Italy)

^c UNIVERSITY OF NEUCHÂTEL   (Switzerland)

Author keywords

Green fluorescent protein; Phaseolin; Plant secretory pathway; Vacuolar sorting

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; FLUORESCENCE; MICROSCOPIC EXAMINATION; PROTEINS;

PRECURSORS;

PLANTS (BOTANY);

EID: 0035034807     PISSN: 01761617     EISSN: None     Source Type: Journal    
DOI: 10.1078/0176-1617-00362     Document Type: Article

References (26)

1. A protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures
2. The barley lectin carboxyl-terminal propeptide is a vacuolar protein sorting determinant in plants
3. Transport of virally expressed green fluorescent protein through the secretory pathway in tobacco leaves is inhibited by cold shock and brefeldin A
4. A precursor of the reserve protein, phaseolin, is transiently associated with the endoplasmic reticulum of developing Phaseolus vulgaris cotyledons
5. Bean homologs of the mammalian glucose regulated proteins: Induction by tunicamycin and interaction with newly synthesized storage proteins in the endoplasmic reticulum
6. Specific accumulation of GFP in a non-acidic vacuolar compartment via a C-terminal propeptide-mediated sorting pathway
7. Regeneration of a lytic central vacuole and of neutral peripheral vacuoles can be visualised by GFP targeted to either type of vacuoles
8. Sorting of phaseolin to the vacuole is saturable and requires a short C-terminal peptide
9. Assembly, secretion and vacuolar delivery of a hybrid immunoglobulin in plants
10. Tonoplast and soluble vacuolar proteins are targeted by different mechanisms
11. Vacuolar storage proteins and the putative vacuolar sorting receptor BP-80 exit the Golgi apparatus of developing pea cotyledons in different transport vesicles
12. Interaction of a potential vacuolar targeting receptor with amino- and carboxyl-terminal targeting determinants
13. The N-terminal propeptide and the C-terminus of the precursor to 20-kilo-dalton potato tuber protein can function as different types of vacuolar sorting signals
14. The green fluorescent protein targets secretory proteins to the yeast vacuole
15. Cis-elements of protein transport to the plant vacuoles
16. Identification of a prevacuolar compartment in stigmas of Nicotiana alata
17. A short C-terminal sequence is necessary and sufficient for targeting of chitinases to the plant vacuole
18. Plant cells contain two functionally distinct vacuolar compartments
19. Protein quality control along the route to the plant vacuole
20. Binding of BiP to an assembly-defective protein in plant cells
21. Different legumin protein domains act as vacuolar targeting signals
22. The vacuolar targeting signal of the 2s albumin from Brazil nut resides at the C-terminus and involves the C-terminal propeptide as an essential element
23. Mutations that suppress the thermosensitivity of green fluorescent protein
24. Brefeldin A effects in plants. Are different Golgi responses caused by different sites of action?
25. Engineering plant protein composition for improved nutrition
26. What do proteins need to reach different vacuoles?