Lack of muscarinic regulation of CA2+ channels in Gi2α gene knockout mouse hearts

American Journal of Physiology - Heart and Circulatory Physiology

Volumn 280, Issue 5 49-5, 2001, Pages

  Chen, Fuhua ^a   Spicher, Karsten ^a   Jiang, Meisheng ^a   Birnbaumer, Lutz ^a   Wetzel, Glenn T ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Ca2+ current regulation; Gene inactivation; Signal transduction

Indexed keywords

ATROPINE; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; CALCIUM CHANNEL L TYPE; CARBACHOL; GUANINE NUCLEOTIDE BINDING PROTEIN; ISOPRENALINE; MUSCARINIC AGENT; MUSCARINIC RECEPTOR; PROTEIN SUBUNIT;

ANIMAL CELL; ARTICLE; CALCIUM CURRENT; CONTROLLED STUDY; GENE INACTIVATION; HEART MUSCLE CELL; HEART VENTRICLE; KNOCKOUT GENE; MOUSE; NONHUMAN; PRIORITY JOURNAL; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; VOLTAGE CLAMP;

EID: 0035026561     PISSN: 03636135     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpheart.2001.280.5.h1989     Document Type: Article

References (28)

1. Abramowitz J, Iyengar R, and Birnbaumer L. Guanine nucleotide and magnesium ion regulation of the interaction of gonadotropic and β-adrenergic receptors with their hormones: a comparative study using a single membrane system. Endocrinology 110: 336-346, 1982.
2. o, a GTP-binding protein: immunochemical and immunohistochemical localization in the rat. J Neurochem 50: 1164-1169, 1988.
3. Balligand JL, Kobzik L, Han X, Kaye DM, Belhassen L, O'Hara DS, Kelly RA, Smith TW, and Michel T. Nitric oxide-dependent parasympathetic signaling is due to activation of constitutive endothelial (type III) nitric oxide synthase in cardiac myocytes. J Biol Chem 270: 14582-14586, 1995.
4. Birnbaumer L and Birnbaumer M. Signal transduction by G proteins: 1994 edition. J Recept Signal Transduct Res 15: 213-252, 1995.
5. Brown AM and Birnbaumer L. Ionic channels and their regulation by G protein subunits. Annu Rev Physiol 52: 197-213, 1990.
6. Chen F, Van Dop C, Wetzel GT, Lee RH, Friedman WF, and Klitzner TS. Adenylyl cyclase integrates multiple G protein signals to modulate calcium currents in neonatal rabbit heart. Biochem Biophys Res Commun 216: 190-197, 1995.
7. 2+ current in isolated neonatal rabbit ventricular myocytes. J Investig Med 44: 583-589, 1996.
8. Chen F, Wetzel GT, Friedman WF, and Klitzner TS. Single channel recording of inwardly rectifying potassium currents in developing myocardium. J Mol Cell Cardiol 23: 259-267, 1991.
9. 2+ currents and contraction in rabbit hearts. J Mol Cell Cardiol 28: 635-642, 1996.
10. Gilman AG. G proteins and regulation of adenylyl cyclase. JAMA 262: 1819-1825, 1989.
11. Hamill OP, Marty A, Neher E, Sakmann B, and Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391: 85-100, 1981.
12. 2+ current in adult rabbit atrioventricular nodal cells. Circ Res 78: 998-1008, 1996.
13. Ca-L is absent in mice with targeted disruption of endothelial nitric oxide synthase. Proc Natl Acad Sci USA 95: 6510-6515, 1998.
14. Han X, Shimoni Y, and Giles WR. An obligatory role for nitric oxide in autonomic control of mammalian heart rate. J Physiol 476: 309-314, 1994.
15. Hildebrandt JD, Sekura RD, Codina J, Iyengar R, Manclark CR, and Birnbaumer L. Stimulation and inhibition of adenylyl cyclases is mediated by distinct proteins. Nature 302: 706-709, 1983.
16. o. Proc Natl Acad Sci USA 95: 3269-3274, 1998.
17. Katada T, Bokoch GM, Northup JK, Ui M, and Gilman AG. The inhibitory guanine nucleotide-binding regulatory component of adenyl cyclase: properties and function of the purified protein. J Biol Chem 259: 3568-3577, 1984.
18. Li Y, Mende U, Lewis C, and Neer EJ. Maintenance of cellular levels of G-proteins: different efficiencies of as and αo synthesis in GH3 cells. Biochem J 318: 1071-1077, 1996.
19. Mattera R, Pitts BJR, Entman ML, and Birnbaumer L. Guanine nucleotide regulation of a mammalian myocardiac muscarinic receptor system. J Biol Chem 260: 7410-7421, 1985.
20. Pang IH and Sternweis PC. Purification of unique α subunits of GTP-binding regulatory proteins (G proteins) by affinity chromatography with immobilized βγ subunits. J Biol Chem 265: 18707-18712, 1990.
21. Robishaw JD and Foster KA. Role of G proteins in the regulation of the cardiovascular system. Annu Rev Physiol 51: 229-244, 1989.
22. i2α locus in embryonic stem cells and mice: a modified hit and run strategy with detection by a PCR dependent on gap repair. Transgenic Res 2: 345-355, 1993.
23. i2α-/-mice. Proc Natl Acad Sci USA 93: 3209-3214, 1996.
24. 2+ channels in mouse heart. Proc Natl Acad Sci USA 94: 1727-1732, 1997.
25. 2+ current in human atrial myocytes. J Physiol 506: 653-663, 1998.
26. Wetzel GT, Chen F, and Klitzner TS. Calcium channel kinetics in acutely isolated fetal neonatal and adult rabbit cardiac myocytes. Circ Res 72: 1065-1074, 1993.
27. 2+ exchange and cell contraction in isolated neonatal rabbit cardiac myocytes. Am J Physiol Heart Circ Physiol 268: H1237-H1733, 1995.
28. o are all required for normal muscarinic inhibition of the cardiac calcium channels in nodal/atrial-like cultured cardiocytes. J Mol Cell Cardiol 31: 1771-1781, 1999.