A basic residue at position 36p of the propeptide is not essential for the correct folding and subsequent autocatalytic activation of prochymosin

European Journal of Biochemistry

Volumn 268, Issue 8, 2001, Pages 2362-2368

  Francky, Andrej ^a   Francky, Bojana Mozetic ^a   Strukelj, Borut ^a   Gruden, Kristina ^a   Ritonja, Anka ^a   Krizaj, Igor ^a   Kregar, Igor ^a   Pain, Roger H ^a   Pungercar, Joze ^a  

^a JO EF STEFAN INSTITUTE   (Slovenia)

Author keywords

Activation; Aspartic proteinase; Prochymosin; Propeptide; Site directed mutagenesis

Indexed keywords

AMINO ACID; CHYMOSIN; COMPLEMENTARY DNA; ENZYME PRECURSOR; GLUTAMIC ACID; MUTANT PROTEIN; PROCHYMOSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ENZYME ACTIVATION; ENZYME ACTIVE SITE; LAMB; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN ISOLATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BINDING SITES; CATALYSIS; CATTLE; CHYMOSIN; CIRCULAR DICHROISM; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME PRECURSORS; ESCHERICHIA COLI; EXONS; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; LYSINE; MILK; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPSINOGEN A; PEPTIDES; POLYMERASE CHAIN REACTION; PROTEIN FOLDING; PROTEIN PRECURSORS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID; SHEEP; TIME FACTORS;

ANIMALIA; CAPRA HIRCUS; CAPRINAE; ESCHERICHIA COLI; OVIS ARIES; OVIS ARIES MUSIMON; SUIDAE;

EID: 0035021776     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02119.x     Document Type: Article

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