Direct transfection and activation of human cutaneous dendritic cells

Gene Therapy

Volumn 8, Issue 8, 2001, Pages 608-617

  Larregina, A T ^a,b   Watkins, S C ^a,b   Erdos, G ^a,b   Spencer, L A ^a,b   Storkus, W J ^a   Beer Stolz, D ^a   Falo Jr , L D ^a,b  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PITTSBURGH CANCER INSTITUTE   (United States)

Author keywords

Dendritic cells; Gene gun; Genetic immunization; Human skin; Langerhans cells; Melanoma

Indexed keywords

ANTIGEN; GOLD; MESSENGER RNA; PEPTIDE; VACCINE;

ANTIGEN EXPRESSION; ARTICLE; BIOLISTIC TRANSFORMATION; CELL ACTIVATION; CELL MIGRATION; CONTROLLED STUDY; DENDRITIC CELL; DRUG DELIVERY SYSTEM; DRUG DESIGN; EPIDERMIS; GENE GUN; GENE TARGETING; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; HUMAN TISSUE; IMMUNE RESPONSE; IMMUNIZATION; LANGERHANS CELL; MELANOMA; PRESSURE; PRIORITY JOURNAL; SKIN; SKIN CULTURE; STIMULATION; TECHNIQUE; TRANSGENE; VACCINE PRODUCTION;

BIOLISTICS; CELL CULTURE TECHNIQUES; CELL MOVEMENT; DENDRITIC CELLS; EPIDERMIS; EPITOPES; GENE THERAPY; GENE TRANSFER TECHNIQUES; GOLD; HUMANS; LANGERHANS CELLS; NEOPLASM PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SKIN; T-LYMPHOCYTES, CYTOTOXIC; TRANSFECTION; TRANSGENES;

EID: 0035013594     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301404     Document Type: Article

References (39)

1. Paving the way towards DNA vaccines
2. Exposing the immunology of naked DNA vaccines
3. The skin immune system (SIS)
4. Dendritic cell loss from non-lymphoid tissue after systemic administration of lipopolysaccharide, tumor necrosis factor, and interleukin 1
5. Dendritic cells and the control of immunity
6. Langerhans cells and chemical allergy
7. DNA-based immunization by in vivo transfection of dendritic cells
8. 8+ T cells after gene gun immunization
9. Expression of naked DNA in human, pig and mouse skin
10. Pattern of cytokine receptors expressed by human dendritic cells migrated from dermal explants
11. Phenotype of Langerhans cells during maturation to veiled cells
12. Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice
13. Differential dependence on target site tissue for gene gun and intramuscular DNA immunizations
14. The skin immune system: Progress in cutaneous biology
15. DNA Immunization targeting the skin: Molecular control of adaptive immunity
16. Targeting the skin for genetic immunization
17. Introduction of foreign genes into tissues of living mice by DNA-coated microprojectiles
18. Induction of tumor-specific immunity using plasmid DNA immunization in mice
19. Route and method of delivery of DNA vaccine influence immune responses in mice and non-human primates
20. Maturation and trafficking of monocyte-derived dendritic cells in monkeys: Implications for dendritic cell-based vaccines
21. Expression and modulation of C5a receptor (CD88) on skin dendritic cells. Chemotatic effect of the C5a on skin migratory dendritic cells
22. Migration and maturation of Langerhans cells in skin transplants and explants
23. Immune activation by bacterial DNA: A new genetic code
24. Gene vaccination: Plasmid DNA is more than just a blueprint
25. Bacterial DNA and CpG-containing oligodeoxynucleotides activate cutaneous dendritic cells and induce IL-12 production: Implications for the augmentation of Th1 responses
26. Antigen presentation by dendritic cells after immunization with DNA encoding a major histocompatibility complex class II-restricted viral epitope
27. Immunization through dermal delivery of protein-encoding DNA: A role for migratory dendritic cells
28. Autologous human monocyte derived dendritic cells modified to express melanoma antigens elicit primary cytotoxic T cell responses in vitro: Enhancement by co-transfection of genes encoding the Th1 biasing cytokines IL-12 and IFN-α
29. Physical interaction between dendritic cells and tumor cells results in an immunogen that induces protective and therapeutic tumor rejection
30. Dendritic cells acquire antigen from apoptotic cells and induce class I-restricted CTLs
31. Cross presentation: A general mechanism for CTL immunity and tolerance
32. Human and murine dermis contain dendritic cells. Isolation by means of a novel method and phenotypical and functional characterization
33. Both dendritic cells and memory T lymphocytes emigrate from organ cultures of human skin and form distinctive dendritic-T-cell conjugates
34. Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells
35. Immunological surveillence of tumours in the context of major histocompatibility restriction of T-cell function
36. Gene vaccination with naked plasmid DNA. Mechanism for CTL primming
37. DNA transfection and activation of dendritic cells as a key events for immunity
38. Flow cytometric analysis of cytokines receptors on human Langerhans cells. Changes observed after short-term culture