Use of heteroduplex mobility assay for identification and differentiation of phytoplasmas in the aster yellows group and the clover proliferation group

Phytopathology

Volumn 91, Issue 6, 2001, Pages 546-552

  Wang, K ^a   Hiruki, C ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALFALFA; ASSAY; CLOVER; CULTIVAR; HETERODUPLEX; INTERGENIC SPACER; MOLECULAR EPIDEMIOLOGY; PHYTOPLASMA; POLYMERASE CHAIN REACTION; POTATO; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; RNA 16S; SEQUENCE ANALYSIS; WITCHES BROOM DISEASE;

BRACHYCOMBE MULTIFIDA; CALENDULA OFFICINALIS; CALLISTEPHUS CHINENSIS; CHRYSANTHEMUM PARTHENIUM; CLARKIA UNGUICULATA; COSMOS BIPINNATUS; DAUCUS CAROTA; ECHINACEA PARPUREA; ESCHSCHOLTZIA CALIFORNICA; HELICHRYSUM BRACTEATUM; MONARDA FISTULOSA; SCABIOSA ATROPURPUREA; TAGETES PATULA; ZINNIA ELEGANS;

EID: 0035012899     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO.2001.91.6.546     Document Type: Article

References (32)

1. Comparative molecular studies on aster yellows phytoplasmas
2. Mycoplasma-like organisms associated with phyllody of Brassica chinensis L.
3. Electron microscopy of mycoplasmalike bodies associated with clover proliferation disease
4. Effects of dark treatment on the ultrastructure of the aster yellows agent in situ
5. Occurrence of tubular structures in Vinca rosea infected with the Alberta isolate of the aster yellows agent
6. Use of heteroduplex mobility analysis (HMA) for differentiating phytoplasma isolates causing witches'-broom disease on Populus nigra cv. italica and stolbur or big bud symptoms on tomato
7. Detection of mycoplasmalike organisms in situ by indirect immunofluorescence microscopy
8. Human immunodeficiency virus type I evolution in vivo tracked by DNA heteroduplex mobility assays
9. Genetic relationships determined by a DNA heteroduplex mobility assay: Analysis of HIV-1 env Genes
10. Enhanced detection of a plant pathogenic mycoplasmalike organism by polymerase chain reaction
11. Amplification of 16S rRNA genes from culturable and non-culturable mollicutes
12. Mycoplasma or P.L.T. group-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches'-broom, aster yellows or paulownia witches'-broom
13. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs
14. Phylogeny of mycoplasmalike organisms (phytoplasmas): A basis for their classification
15. Yellows diseases of calendula (Calendula officinalis) and valerian (Valeriana officinalis) in Alberta, Canada, associated with phytoplasma infection
16. First report of a yellows phytoplasma disease in purple coneflower (Echinacea spp.) in Canada
17. The molecular genetic relatedness of willow witches'-broom phytoplasma to the clover proliferation group
18. Molecular detection of alfalfa witches'-broom phytoplasma in four leafhopper species associated with infected alfalfa plants
19. Molecular identification of aster yellows phytoplasma in purple coneflower and monarda based on PCR amplification and RFLP analyses of 16S rDNA sequences
20. Phylogenetic relationships between the western aster yellows mycoplasmalike organism and other prokaryotes established by 16S rRNA gene sequence
21. Genetic interrelatedness among clover proliferation mycoplasmalike organisms (MLOs) and other MLOs investigated by nucleic acid hybridization and restriction fragment length polymorphism analyses
22. Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms
23. Plant diseases associated with mycoplasmalike organisms
24. Classification of plant-pathogenic mycoplasma-like organisms using restriction-site analysis of PCR-amplified 16S rDNA
25. Current status of molecular classification of the phytoplasmas
26. Phylogenetic classification of phytopathogenic mollicutes by sequence analysis of 16S ribosomal DNA
27. Phytoplasma-specific PCR primers based on sequences of the 16-23S rRNA spacer region
28. Purple coneflower is a host of the aster yellows phytoplasma
29. Rapid detection of a single-base-pair mutation in phytoplasmal conserved genes by DNA heteroduplex mobility assay (HMA)
30. Heteroduplex mobility assay (HMA) detects DNA mutations for differentiation of closely related phytoplasma strains
31. Identification of a phytoplasma causing yellows of Monarda
32. Genetic differentiation of phytoplasma isolates determined by a DNA heteroduplex mobility assay