Molecular identification of aster yellows phytoplasma in purple coneflower and monarda based on PCR amplification and RFLP analyses of 16S rDNA sequences

Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz

Volumn 104, Issue 4, 1997, Pages 403-410

  Khadhair, A H ^a   Hwang, S F ^a   Chang, K F ^b   Howard, R J ^b  

^a ALBERTA RESEARCH COUNCIL   (Canada)

^b Crop Diversification Centre South   (Canada)

Author keywords

Aster yellows; Medicinal plants; Mollicutes; Mycoplasma like organisms; PCR; Phytoplasma; RFLP; Yellows diseases

Indexed keywords

MONARDA;

EID: 0030821198     PISSN: 03408159     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. AHRENS, U., E. SEEMÜLLER: Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. - Phytopathology 82, 828-832, 1992.
2. CHIYKOWSKI, L. N.: Clover phyllody virus in Canada and its transmisssion. - Can. J. Bot. 40, 397-404, 1962.
3. CHIYKOWSKI, L. N.: A yellows-type virus of alsike clover in Alberta. - Can. J. Bot. 43, 527-536, 1965.
4. DAVIS, R. E., I.-M. LEE, S. M. DOUGLAS, E. L. DALLY: Molecular cloning and detection of chromosomal and extrachromosomal DNA of the mycoplasma-like organism associated with little disease in periwinkle (Catharanthus roseus). - Phytopathology 80, 789-793, 1990.
5. DENG, S., C. HIRUKI: Genetic relatedness between two nonculturable mycoplasma-like organisms revealed by nucleic acid hybridization and polymerase chain reaction. - Phytopathology 81, 1475-1479, 1991a.
6. DENG, S., C. HIRUKI: Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. - J. Microbiol. Method. 14, 53-61, 1991b.
7. FEGHAHATI, S. M. J., R. N. REESE: Ethylene-, light-, and prechill-enhanced germination of Echinacea angustifolia seeds. -J. Amer. Soc. Hort. Sci. 119, 853-858. 1994.
8. GUNDERSEN, D. E., I.-M. LEE, S. A. REHNER, R. E. DAVIS, D. T. KINGSBURY: Phylogeny of mycoplasma-like organisms (Phytoplasmas): A Basis for their classification. - J. Bact. 176, 5244-5254, 1994.
9. HWANG, S. F., K. F. CHANG, R. J. HOWARD, A. H. KHADHAIR, R. G. GAUDIEL, C. HIRUKI: First report of a yellows disease of purple coneflower (Echinacea spp.) caused by a phytoplasma. - J. Plant Diseases and Protection 102, 182-192, 1997.
10. KHADHAIR, A. H., C. HIRUKI: The molecular genetic relatedness of willow witches'-broom phytoplasma to clover proliferation group. - Proc. Japan Acad. 71B, 145-147, 1995.
11. KUSKE, C. R., B. C. KIRKPATRICK: Distribution and multiplication of Western aster yellows mycoplasmalike organisms in Catharanthus roseus as determined by DNA hybridization analysis. - Phytopathology 82, 457-462, 1992a.
12. KUSKE, C. R., B. C. KIRKPATRICK: Phylogenetic relationships between the western aster yellows mycoplasmalike organisms and other prokaryotes established by 16S rRNA gene sequence. - Int. J. Syst. Bacteriol. 42, 226-233, 1992b.
13. LEE, I.-M., R. E. DAVIS: Detection and investigation of genetic relatedness among aster yellows and other mycoplasmalike organisms by using cloned DNA and RNA probes. - Mol. Plant-Microbe Interact. 1, 303-310, 1988.
14. LEE, I.-M., R. E. DAVIS, C. HIRUKI: Genetic relatedness among clover proliferation mycoplasmalike organisms (MLOs) and other MLOs investigated by nucleic acid hybridizations and restriction fragment length polymorphism analyses. - Appl. Environ. Microbiol. 57, 3565-3569, 1991.
15. LEE, I.-M., D. E. GUNDERSEN, R. W. HAMMOND, R. E. DAVIS: Use of mycoplasmalike organisms (MLOs) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. - Phytopathology 84, 559-566, 1994.
16. LEE, I.-M., R. W. HAMMOND, R. E. DAVIS, D. E. GUNDERSEN: Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasmalike organisms. - Phytopathology 83, 834-842, 1993.
17. LIM, P. O., B. B. SEARS: 16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionary distinct from animal mycoplasmas. - J. Bact. 171, 5901-5906, 1989.
18. MCCOY, R. E., A. CAULDWELL, C. J. CHANG, T. A. CHEN, L. N. CHIYKOWSKI, M. T. COUSIN, J. L. DALE, G. T. N. DE LEEUW, D. A. GOLINO, K. J. HACKETT, B. C. KIRKPATRICK, R. MARWITZ, H. PETZOLD, R. C. SINHA, M. SUGIURA, R. F. WHITCOMB, I. L. YANG, B. M. ZHU, E. SEEMÜLLER: Plant diseases associated with mycoplasma-like organisms. - In: WHITCOMB, R. F., J. G. TULLY (eds.): The Mycoplasma, Vol. V. Spiroplasma, acholeplasmas, and mycoplasmas of plants and arthropods, pp. 545-640. Academic Press, San Diego, 1989.
19. MURRAY, M. G., W. F. THOMPSON: Rapid isolation of high molecular weight plant DNA. - Nucleic Acids Res. 8, 4321-4326, 1980.
20. SCHNEIDER, B., U. AHRENS, B. C. KIRKPATRICK, E. SEEMÜLLER: Classification of plant-pathogenic mycoplasma-like organisms using restriction-site analysis of PCR-amplified 16S rDNA. - J. gen. Microbiol. 139, 519-527, 1993.