Human liver-specific organic anion transporter, LST-1, mediates uptake of pravastatin by human hepatocytes

Journal of Pharmacology and Experimental Therapeutics

Volumn 297, Issue 3, 2001, Pages 861-867

  Nakai, Daisuke ^a   Nakagomi, Rie ^a   Furuta, Yoshitake ^a   Tokui, Taro ^a   Abe, Takaaki ^b   Ikeda, Toshihiko ^a   Nishimura, Kenji ^a  

^a DAIICHI SANKYO CO   (Japan)

^b TOHOKU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOHIPPURIC ACID; ANTISENSE OLIGODEOXYNUCLEOTIDE; BROMSULFOPHTHALEIN; CARRIER PROTEIN; CHOLESTEROL; ESTRADIOL 17 GLUCURONIDE; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; LIVER SPECIFIC ORGANIC ANION TRANSPORTER 1; POLYADENYLATED RNA; PRAVASTATIN; SIMVASTATIN; SODIUM; TAUROCHOLIC ACID; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHOLESTEROL SYNTHESIS; COMPETITIVE INHIBITION; CONTROLLED STUDY; DRUG UPTAKE; FEMALE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; LIVER CELL; MICHAELIS CONSTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TEMPERATURE DEPENDENCE; XENOPUS;

ANIMALS; ANION TRANSPORT PROTEINS; BIOLOGICAL TRANSPORT; CARBON RADIOISOTOPES; CARCINOMA, HEPATOCELLULAR; CARRIER PROTEINS; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ESTRADIOL; FEMALE; GLUCURONIDES; HEPATOCYTES; HUMANS; HYDROXYMETHYLGLUTARYL-COA REDUCTASE INHIBITORS; IMMUNOHISTOCHEMISTRY; OOCYTES; PRAVASTATIN; TAUROCHOLIC ACID; TRITIUM; XENOPUS LAEVIS;

EID: 0035012075     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Abe T, Kakyo M, Sakagami H, Tokui T, Nishio T, Tanemoto M, Nomura H, Hebert SC, Matsuno S, Kondo H, et al. (1998) Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholic acid, and comparison with oatp2. J Biol Chem 273:22395-22401.
2. Abe T, Kakyo M, Tokui T, Nakagomi R, Nakai D, Nomura H, Unno M, Suzuki M, Naitoh T, Matsuno S, et al. (1999) Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1. J Biol Chem 274:17159-17183.
3. Azer SA and Stacy NH (1993) Differential effects of cyclosporin A on the transport of bile acids by human hepatocytes. Biochem Pharmacol 46:813-819.
4. Berk PD, Potter BJ and Stremmel W (1987) Role of plasma membrane ligand-binding proteins in the hepatocellular uptake of albumin-bound organic anions. Hepatology 7:165-176.
5. Cohen LH, van Vliet A, Roodenburg L, Jansen LMC and Griffioen M (1993) Pravastatin inhibited the cholesterol synthesis in human hepatoma cell line Hep G2 less than simvastatin and lovastatin, which is reflected in the upregulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and squalene synthase. Biochem Pharmacol 45:2203-2208.
6. De Loecker R, Fuller BJ, Gruwez J and De Loecker W (1990) The effects of cryopreservation on membrane integrity, membrane transport, and protein synthesis in rat hepatocytes. Cryobiology 27:143-152.
7. Goldin A (1992) Maintenance of Xenopus laevis and oocyte injection. Methods Enzymol 207:266-279.
8. Groothuis GMM, Sandker GW, Prium J, Weert B, Slooff MJH and Meijer DKF (1995) Comparison of human hepatocytes isolated from livers accepted or discarded for orthotopic transplantation. Toxicol In Vitro 9:951-958.
9. +/bile acid cotransporter. J Clin Invest 93:1326-1331.
10. +/bile acid cotransport system. Proc Natl Acad Sci USA 88:10629-10633.
11. Hamelin BA and Turgeon J (1998) Hydrophilicity/lipophilicity: relevance for the pharmacology and clinical effects of HMG-CoA reductase inhibitors. Trends Pharmacol Sci 19:26-37.
12. Hsiang B, Zhu Y, Wang Z, Wu Y, Sasseville V, Yang WP and Kirchgessner TG (1999) A novel human hepatic organic anion transporting polypeptide (OATP2). J Biol Chem 274:37161-37168.
13. Koga T, Shimada Y, Kuroda M, Tsujita Y, Hasegawa K and Yamazaki M (1986) Tissue selective inhibition of cholesterol synthesis in vivo by pravastatin sodium, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor. Biochim Biophys Acta 877:50-60.
14. Komai T, Shigehara E, Tokui T, Koga T, Ishigami M, Kuroiwa C and Horiuchi S (1992) Carrier-mediated uptake of pravastatin by rat hepatocytes in primary culture. Biochem Pharmacol 43:667-670.
15. König J, Cui Y, Nies AT and Keppler D (2000) A novel human organic anion transporting polypeptide localized to the basolateral hepatocyte membrane. Am J Physiol 278:G156-G164.
16. Kullak-Ublick GA, Hagenbuch B, Stieger B, Schteingart CD, Hofmann AF, Wolkoff AW and Meier PJ (1995) Molecular and functional characterization of an organic anion transporting polypeptide cloned from human liver. Gastroenterology 109: 1274-1282.
17. Lu R, Kanai N, Bao Y and Schuster V (1996) Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA (hPGT). J Clin Invest 98:1142-1149.
18. Meier PJ (1988) Transport polarity of hepatocytes. Semin Liver Dis 8:649-661.
19. Müller M and Jansen PL (1997) Molecular aspects of hepatobiliary transport. Am J Physiol 272:G1285-G1303.
20. Sandker GW, Weert B, Olinga P, Wolters H, Slooff MJH, Meijer DKF and Groothuis GMM (1994) Characterization of transport in isolated human hepatocytes. Biochem Pharmacol 47:2193-2000.
21. Scott WA, Mahoney EM and Mosley ST (1989) Mechanism of action and differential pharmacology of pravastatin, a hydrophilic and selective HMG-CoA reductase inhibitor, in New Advances in the Control of Lipid Metabolism: Focus on Pravastatin (LaRosa JC ed) pp 1-8, Royal Society of Medicine, London.
22. Sekine T, Watanabe N, Hosoyamada M, Kanai Y and Endou H (1997) Expression cloning and characterization of a novel multispecific organic anion transporter. J Biol Chem 272:18526-18529.
23. Shigemoto R, Abe T, Nomura S, Nakanishi S and Hirano T (1994) Antibodies inactivating mGluR1 metabotropic glutamate receptor block long-term depression in cultured Purkinje cells. Neuron 12:1245-1255.
24. Tiribelli C, Lunazzi GC and Sottocasa GL (1990) Biochemical and molecular aspects of the hepatic uptake of organic anions. Biochim Biophys Acta 1031:261-275.
25. Tokui T, Nakai D, Nakagomi R, Yawo H, Abe T and Sugiyama Y (1999) Pravastatin, an HMG-CoA reductase inhibitor, is transported by rat organic anion transporting polypeptide, oatp2. Pharm Res 16:904-908.
26. Tsujita Y, Kuroda M, Shimada Y, Tanzawa K, Arai M, Kaneko I, Tanaka M, Masuda H, Tamura C and Watanabe Y (1990) CS-514, a competitive inhibitor of 3-hydroxyl-3-methylglutaryl coenzyme A reductase: tissue-selective inhibition of sterol synthesis and hypolipidemic effect on various animal species. Biochim Biophys Acta 1045:115-120.
27. van Vliet AK, van Thiel GCF, Huisman RH, Moshage H, Yap SH and Cohen LH (1995) Different effects of 3-hydroxyl-3-methylglutaryl-coenzyme A reductase inhibitors on sterol synthesis in various human cell types. Biochim Biophys Acta 1254:105-111.
28. +-independent multispecific anion transporter mediates active transport of pravastatin into rat liver. Am J Physiol 264:G36-G44.
29. Ziegler K, Blumrich M and Hummelsiep S (1994) The transporter for the HMG-CoA reductase inhibitor pravastatin is not present in Hep G2 cells. Evidence for the nonidentity of the carrier for pravastatin and certain transport for BSP. Biochim Biophys Acta 1223:195-201.