Site-directed mutagenesis of human membrane-associated ganglioside sialidase: Identification of amino-acid residues contributing to substrate specificity

European Journal of Biochemistry

Volumn 268, Issue 8, 2001, Pages 2201-2208

  Wang, Yan ^a,b   Yamaguchi, Kazunori ^a   Shimada, Yukiko ^a   Zhao, Xuejian ^b   Miyagi, Taeko ^a  

^a MIYAGI CANCER CENTER RESEARCH INSTITUTE   (Japan)

^b JILIN UNIVERSITY   (China)

Author keywords

Gangliosides; Sialic acid; Sialidase; Site directed mutagenesis; Substrate specificity

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; GANGLIOSIDE; SIALIC ACID; SIALIDASE;

AMINO ACID ANALYSIS; ANIMAL CELL; ARTICLE; ENZYME ASSAY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SALMONELLA TYPHIMURIUM; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; ARGININE; ASPARTIC ACID; BINDING SITES; BLOTTING, WESTERN; CELL MEMBRANE; COS CELLS; GANGLIOSIDES; GLUTAMIC ACID; GLUTAMINE; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NEURAMINIDASE; RATS; SALMONELLA TYPHIMURIUM; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TEMPERATURE; TRANSFECTION;

ANIMALIA; MAMMALIA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0035007030     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02069.x     Document Type: Article

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