Superfluous transgene integration in plants

Critical Reviews in Plant Sciences

Volumn 20, Issue 3, 2001, Pages 215-249

  Smith, Natasha ^a   Kilpatrick, John B ^a   Whitelam, Garry C ^b  

^a ADAS BOXWORTH   (United Kingdom)

^b UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Agrobacterium tumefaciens; Agrolistics; GMOs; Microprojectile bombardment; Plant transformation; Superfluous DNA

Indexed keywords

DNA SEQUENCE; DNA TRANSFER; DNA VECTOR; GENE INSERTION; GENETIC TRANSFORMATION; HOMOLOGY; MACROGOL; MICROPROJECTILE BOMBARDMENT; MOLECULAR EVOLUTION; PLANT BIOTECHNOLOGY; PROTOPLAST; TRANSGENIC PLANT;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; EMBRYOPHYTA; RADIOBACTER; RHIZOBIUM;

EID: 0034990147     PISSN: 07352689     EISSN: None     Source Type: Journal    
DOI: 10.1080/20013591099218     Document Type: Review

References (201)

1. DNA uptake during electroporation of germinating pollen grains
2. Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome
3. Organ-specific and developmental regulation of the nopaline synthase promoter in transgenic tobacco plants
4. New cloning vehicles for transformation of higher plants
5. Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment
6. Tetracycline resistance determined by pBR322 is mediated by one polypeptide
7. Regeneration of intact tobacco plants containing full-length copies of genetically engineered T-DNA, and transmission of T-DNA to R1 progeny
8. Fertile transgenic wheat from microprojectile bombardment of scutellar tissue
9. Why do chloroplasts and mitochondria contain so many copies of their genome?
10. The CaMV 35S enhancer contains at least two domains which can confer developmental and tissue-specific expression patterns
11. A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation
12. Binary Agrobacterium vectors for plant transformation
13. Principles of gene transfer using particle bombardment
14. Competence for gene transfer by electroporation in a subpopulation of protoplasts from uniform carrot cell suspensions
15. Recovery of transgenic peanut (Arachis hypogaea L.) plants from elite cultivars utilizing ACCELL technology
16. Bacterial conjugation protein MobA mediates integration of complex DNA structures into plant cells
17. Removal of selectable marker genes from transgenic plants: Needless sophistication or social necessity?
18. Reply from Bryant and Leather
19. The mob and ori T functions of a bacterial plasmid promote its transfer to plants
20. Transkingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae
21. Integration of Agrobacterium-tumefaciens T-DNA in the Saccharomyces cerevisiae genome by illegitimate recombination
22. An ethanol inducible gene switch for plants used to manipulate carbon metabolism
23. Altering the genome by homologous recombination
24. Genetic analysis of integration mediated by single T-DNA borders
25. Targeted insertion of foreign genes into the tobacco plastid genome without physical linkage to the selectable marker gene
26. Genetic transformation of wheat mediated by Agrobacterium tumefaciens
27. Stable transformation of soybean callus by DNA-coated gold particles
28. Inheritance and expression of foreign genes in transgenic soybean plants
29. Cotransformation frequencies of foreign genes in soybean cell cultures
30. The development of a variety-independent gene transfer method for rice
31. Transformation technology
32. Cooperation interaction of Agrobacterium VirE2 protein with single stranded DNA: Implications for the T-DNA transfer process
33. The molecular structure of Agrobacterium VirE2 - Single stranded DNA complexes involved in nuclear import
34. Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression
35. Quantification of DNAs by the polymerase chain reaction using an internal control
36. Molecular and genetic characterization of elite transgenic rice plants produced by electric-discharge particle acceleration
37. Use of site-specific recombination to regenerate selectable markers
38. Biochemical-characterization of a spearmint mutant that resembles peppermint in monoterpene content
39. Fate of selectable marker DNA integrated into the genome of Nicotiana tabacum
40. Gene Transfer with subsequent removal of the selection gene from the host genome
41. The effect of selection pressure on transformation frequency and copy number in transgenic plants of tall fescue (Festuca arundinacea Schreb.)
42. Transgenic plants of Lolium multiflorum, Lolium perenne, Festuca arundinacea, and Agrostis stolonifera by silicon carbide fibre-mediated transformation of cell suspension cultures
43. Containment of herbicide resistance through genetic engineering of the chloroplast genome
44. Two T-DNAs co-transformed into Brassica napus by a double Agrobacterium infection are mainly integrated at the same locus
45. Agrobacterium tumefaciens-mediated transformation of filamentous fungi
46. T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs
47. Broad host range DNA cloning system for Gram-negative bacteria. Construction of a gene bank of Rhizobium meliloti
48. Stable transformation of maize with a chimaeric, modified phosphinotrycin-acyltransferase gene from Streptomyces viridochromogenes
49. Ti-plasmid homolgous sequences present in tissues from Agrobacterium plasmid-transformed Petunia protoplasts
50. The amount of input DNA affects phenotypic variation in tobacco transformed by the particle gun
51. Isolation of the transposable maize controlling elements Ac and Ds
52. Transformation of cotton (Gossypium hirsutum L.) via particle bombardment
53. Secondary metabolism and the risks of GMOs
54. Selectable markers genes: Safe for plants?
55. Production of fertile transgenic maize plants by silicon carbide whisker-mediated transformation
56. Identification of unexplained DNA fragments within the T-DNA borders of the Bin 19 transformation vector
57. Complete sequence of the binary vector Bin 19
58. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants
59. Safety assessment of the neomycin phosphotransferase II (NPTII) protein
60. Novel high-and low-copy stable cosmids for use in Agrobacterium and Rhizobium
61. Chemically inducible promoters in transgenic plants
62. Chemical control of gene expression
63. Hybrid genes in the analysis of transformation conditions. III. Temporal-spatial fate of NPTII gene integration its inheritance and factors affecting these processes in Nicotiana plumbaginifolia
64. Illegitimate recombination in plants: A model for T-DNA integration
65. Marker gene removal: A practical necessity?
66. Transposition mediated re-positioning and subsequent elimination of marker genes from transgenic tomato
67. Transformation of maize cells and regeneration of fertile transgenic plants
68. Indiscriminate use of selectable markers -sowing wild oats?
69. Single-copy T-DNA insertions in Arabidopsis are the predominant form of integration in root-derived transgenics, whereas multiple insertions are found in leaf discs
70. Transformation of 12 different plasmids into soybean via particle bombardment
71. Stable transformation of sorgum cell cultures after bombardment with DNA-coated microprojectiles
72. Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts
73. The small versatile pPZP family of Agrobacterium binary vectors for plant transformation
74. "Agrolistic" transformation of plant cells: Integration of T-strands generated in planta
75. T-strand integration in maize protoplasts after codelivery of a T-DNA substrate and virulence genes
76. The effect of right terminal repeat deletion on the oncogenicity of the T-region of pTiT37
77. Expression of chimeric genes transferred into plant cells using a Ti-plasmid-derived vector
78. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA
79. Transformation of yeast
80. A binary plant vector strategy based on separation of vir- and T-regions of the Agrobacterium tumefaciens Ti-plasmid
81. New Agrobacterium helper plasmids for gene transfer to plants
82. Efficiency of particle-bombardment-mediated transformation is influenced by cell cycle stage in synchronized cultured cells of tobacco
83. Analysis of mannose selection used for transformation of sugar beet
84. Parameters interacting with mannose selection employed for the production of transgenic sugar beet
85. Genetic analysis of T-DNA transcripts in nopaline crown galls
86. Genetic analysis of transfer and stabilisation of Agrobacterium DNA in plant cells
87. T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives
88. Silicon carbide fiber-mediated DNA delivery into plant cells
89. Silicon carbide fiber-mediated stable transformation of plant cells
90. Molecular analysis of transgenic plants derived from transformations of protoplasts at various stages of the cell cycle
91. Targeted disruption in Arabidopsis
92. High velocity microprojectiles for delivering nucleic acids into living cells
93. Genetic transformation of maize cells by particle bombardment
94. Enhancement of transformation rates in higher plants by low-dose irradiation - Are DNA repair systems involved in the incorporation of exogenous DNA into the plant genome
95. Effect of various irradiation treatments of plant protoplasts on the transformation rates after direct gene transfer
96. Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots
97. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers
98. High-frequency T-DNA-mediated gene tagging in plants
99. Integration of T-DNA binary vector 'backbone' sequences into the tobacco genome: Evidence for multiple complex patterns of integration
100. In vitro transformation of plant protoplasts with Ti-plasmid DNA
101. Transformation of Dendrobium orchid using particle bombardment of protocorms
102. Tagging genomic sequences that direct transgene expression by activation of a promoter trap in plants
103. Transformation in cultures of Linum usitatissium and L. suffruticosum mediated with PEG and with Agrobacterium tumefaciens
104. Microprojectile mediated plant transformation: A bibliographical search
105. FLP-mediated recombination of FRT sites in the maize genome
106. The Gin recombinase of phage Mu can catalyse site-specific recombination in plant protoplasts
107. Marker gene controversy in transgenic plants
108. Improved efficiency for T-DNA-mediated transformation and plasmid rescue in Arabidopsis thaliana
109. On defining T-DNA
110. In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens
111. Facile transformation of Arabidopsis
112. Expression of a foreign gene in electroporated pollen grains of tobacco
113. Site-specific insertion of genes into T-DNA of the Agrobacterium tumor-inducing plasmid:an approach to genetic engineering of higher plant cells
114. T-DNA integration: A mode of illegitimate recombination in plants
115. Improved binary vectors for Agrobacterium-mediated plant transformation
116. Amplification of a chimeric Bacillus gene in chloroplasts leads to an extraordinary level of an insecticidal protein in tobacco
117. Segregation of genes transferred to one plant from two separate Agrobacterium strains
118. Homology-dependent gene silencing in plants
119. Agrobacterium tumefaciens transfers extremely long T-DNAs by a unidirectional mechanism
120. An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration
121. Differences in susceptibility of Arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration
122. Agrobacterium tumefaciens transformation of the radiation hypersensitive Arabidopsis thaliana mutants uvh1 and rad5
123. Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium
124. Early transcription of Agrobacterium T-DNA genes in tobacco and maize
125. Identification of a brazil-nut allergen in transgenic soybeans
126. Extrachromasomal homologous recombination and gene targeting in plant cells after Agrobacterium mediated transformation
127. Construction and expression in tobacco of a beta-glucuronidase (GUS) reporter gene containing an intron within the coding sequence
128. Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells
129. Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region
130. Selectable marker genes engineered for specific expression in target cells for plant transformation
131. Gene targeting in plants
132. Direct gene transfer to plants
133. Transformation of Rhododendron spp using Agrobacterium tumefaciens with a GUS-intron chimeric gene
134. Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA
135. Revised sequence of the tetracycline-resistance gene of pBR322
136. Transformation of plant protoplasts with DNA: Cotransformation of non-selected calf thymus carder DNA and meiotic segregation of transforming DNA sequences
137. T-DNA border sequences required for crown gall tumorigenesis
138. Sequences signaling T-DNA ends on the Agrobacterium tumefaciens tumor-inducing plasmid
139. Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumor-inducing plasmid
140. Host range implications of plant infection by Agrobacterium rhizogenes
141. Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer
142. Gene transfer to plants: Assessment of published approaches and results
143. Toward targeted transformation in plants
144. L-DNA
145. Agrobacterium tumefaciens and interkingdom genetic exchange
146. Structure and function of selectable and nonselectable transgenes in maize after introduction by particle bombardment
147. Stable transformation of tobacco by electroporation: Evidence for plasmid concatenation
148. Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome
149. Targeted recombination in plants using Agrobacterium coincides with additional rearrangements at the target locus
150. Molecular characterisation of the vir regulon of Agrobacterium tumefaciens: Complete nucleotide sequence and gene organization of the 28.63 kbp regulon cloned as a single unit
151. An efficient and reliable method for determining the number of transgenes inserted into transgenic plants
152. Delivery of substances into cells and tissues using a particle bombardment process
153. Factors influencing Agrobacterium tumefaciens mediated transformation and expression of kanamycin resistance in pickling cucumber
154. Direct gene transfer to protoplasts: Fate of the transferred genes
155. Micro-targetting: High-efficency gene transfer using a novel approach for the acceleration of microprojectiles
156. Efficient gene targeting in the moss Physcomitrella patens
157. Initiation of Agrobacterium tumefaciens T-DNA processing
158. Transformation of alfalfa (Medicago sativa L.) plants with gus marker containing intron
159. The right hand copy of the nopaline Ti plasmid 25 bp repeat is required for tumour formation
160. Multiple-size plasmids in Agrobacterium radiobacter and A. tumefaciens
161. Agrobacterium-plant cell DNA transport: Have virulence proteins, will travel
162. Advances in alternative DNA delivery techniques
163. Arabidopsis mutants deficient in T-DNA integration
164. Segregation of transgenes in maize
165. Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: Requirement for 5′ virD gene products
166. Sequesterpene synthases from grand fir (Abies grandis) -Comparison of constitutive and wound-induced activities, and cDNA isolation, characterization and bacterial expression of delta-selinene synthase and gamma-humulene synthase
167. Complete nucleotide sequence of the Escherichia coli plasmid pBR322
168. The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome
169. The integration of T-DNA into plant genomes
170. Maize transformation utilizing silicon carbide whiskers: A review
171. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection
172. Construction of an intron-containing marker gene-splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation
173. Deviating T-DNA transfer from Agrobacterium tumefaciens to plants
174. Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens
175. Stable transformation and long-term expression of the gusA reporter gene in callus lines of perennial rye grass (Lolium perenne L.)
176. Overdrive is a T-region transfer enhancer which stimulates T-strand production in Agrobacterium tumefaciens
177. Functional analysis of the Agrobacterium tumefaciens octopine Ti plasmid left and right T-region border fragments
178. Studies on the introduction and mobility of the maize activator element in Arabidopsis thaliana and Daucus carota
179. Rapid screening for host-bacterium interactions in Agrobacterium-mediated gene transfer to Chrysanthemum, by using the GUS-intron gene
180. Stably transformed callus lines from microprojectile bombardment of cell suspension cultures of wheat
181. Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus
182. Regeneration of plants from embryonic suspension culture protoplasts of wheat (Triticum aestivum L.)
183. Virulence genes, borders and overdrive generate single-stranded T-DNA molecules from the A6 Ti plasmid of Agrobacterium tumefaciens
184. Cre/lox site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana by transient expression of cre
185. Site-specific integration of Agrobacterium T-DNA in Arabidopsis thaliana mediated by Cre recombinase
186. Generation of large numbers of independently transformed fertile barley plants
187. Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome
188. Site-specific nick occurs within the 25 bp transfer promoting border sequence following induction of vir gene expression in Agrobacterium tumefaciens
189. Sequence context of the T-DNA border repeat element determines its relative activity during T-DNA transfer to plant cells
190. Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum)
191. Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation
192. Evidence that more than 90% of β-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus
193. Biolistic transformation of haploid isolated microspores of barley (Hordeum vulgare L.)
194. A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure
195. Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity
196. Basic processes underlying Agrobacterium-mediated DNA transfer to plant cells
197. Chronicles from the Agrobacterium-plant cell DNA transfer story
198. The basis of crown gall tumorigenesis
199. The Agrobacterium DNA transfer complex