Chemically inducible promoters in transgenic plants

Current Opinion in Biotechnology

Volumn 7, Issue 2, 1996, Pages 168-172

  Gatz, Christiane ^a  

^a BIELEFELD UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

COPPER; DEXAMETHASONE; SALICYLIC ACID; TETRACYCLINE; TRANSCRIPTION FACTOR;

GENE EXPRESSION REGULATION; HIGHER PLANT; PRIORITY JOURNAL; PROMOTER REGION; REVIEW; TRANSGENIC PLANT;

ARABIDOPSIS THALIANA; EMBRYOPHYTA; INSECTA;

EID: 0029924094     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(96)80008-5     Document Type: Article

References (27)

1. Gatz C, Quail PH: Tn10-encoded tet repressor can regulate an operator-containing plant promoter. Proc Natl Acad Sci USA 1988. 85:1394-1397.
2. Hillen W, Berens C: Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu Rev Microbiol 1994, 48:345-369.
3. Frohberg C, Heins L, Gatz C: Characterization of the interaction of plant transcription factors using a bacterial repressor protein. Proc Natl Acad Sci USA 1991, 88:10470-10474.
4. Heins L, Frohberg C, Gatz C: The Tn10 encoded Tet repressor blocks early but not late steps of assembly of the RNA polymerase II inititation complex in vivo. Mol Gen Genet 1992, 232:328-331.
5. Gatz C, Frohberg C, Wendenburg R: Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants. Plant J 1992, 2:397-404.
6. Gatz C, Kaiser A, Wendenburg R: Regulation of a modified CaMV 35S promoter by the Tn10-encoded-Tet repressor in transgenic tobacco. Mol Gen Genet 1991, 227:229-237.
7. Gatz C: Novel inducible/repressible gene expression systems. Methods Cell Biol 1995, 50:411-424.
8. Röder FT, Schmülling T, Gatz C: Efficiency of the tetracycline dependent gene expression system - complete suppression and efficient induction of the RoIB phenotype in transgenic plants. Mol Gen Genet 1994, 243:32-38. The rolB gene is studied as an example of a transgene that, when highly expressed, interferes with the regeneration process. The tetracycline-inducible promoter is shown to exhibit sufficiently tight control to suppress growth inhibition and necrosis; the application of tetracycline induces a severe phenotype.
9. Rieping M, Fritz M, Prat S, Gatz C: A dominant negative mutant of PG13 suppresses transcription from a cauliflower mosaic virus 35S truncated promoter in transgenic tobacco plants. Plant Cell 1994, 6:1087-1098. Expression of a dominant-negative mutant of transcription factor PG13, which leads to reduced amounts of DNA-binding complex ASF-1 in transgenic plants, is placed under the control of the tetracycline-inducible promoter. Use of the tetracycline regulatory system helps to show that the activity of the truncated CaMV 35S promoter is dependent on the presence of ASF-1. Low expression of the truncated CaMV 35S promoter does not result from position effects in these plants. In the presence of tetracycline, expression is low because the dominant-negative PG13 derivative is induced in the absence of tetracycline, expression is high because the dominant-negative PG13 mutant is not expressed.
10. Gossen M, Bonin AL, Bujard H: Control of gene activity in higher eukaryotic cells by prokaryotic regulatory elements. Trends Biochem Sci 1993, 18:471-475.
11. Wilde RJ, Shufflebottom E, Cooke S, Jasinska I, Merryweather A, Beri R, Brammar WJ, Bevan MW, Schuch W: Control of gene expression in tobacco cells using a bacterial operator-repressor system. EMBO J 1992, 11:1251-1259.
12. Picard D, Schena M, Yamamoto R: An inducible expression vector for both fission and budding yeast. Gene 1990, 257-261.
13. Schena M, Lloyd AM, Davis RW: A steroid-inducible gene expression system for plant cells. Proc Natl Acad Sci USA 1991, 88:10421-10425.
14. Lloyd AM, Schena M, Walbot V, Davis RW: Epidermal cell fate determination in Arabidopsis: patterns defined by a steroid-inducible regulator. Science 1994, 266:436-439. A fusion protein consisting of maize transcriptional activator R and the ligand-binding domain of GR is expressed in the Arabidopsis mutant ttg. Thus, an Arabidopsis plant is created containing a steroid hormone dependent conditional allele of R. The response of the chimaeric protein to pulses of hormone is used to define the pattern and timing of trichome formation on the developing leaf epidermis.
15. Melt VL, Lochhead LP, Reynolds PHS: Copper controllable gene expression system for whole plants. Proc Natl Acad Sci USA 1993, 90:4567-4571.
16. Gossen M, Bujard H: Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 1992, 89:5547-5551.
17. Weinmann P, Gossen M, Hillen W, Bujard H, Gatz C: A chimeric transactivator allows tetracycline-responsive gene expression in whole plants. Plant J 1994. 5:559-569. This work describes the function of the tTA system in transgenic plants. The advantages and disadvantages of the two tetracycline-dependent expression systems are discussed.
18. Zeidler M, Gatz C, Hartmann E, Hughes J: Tetracycline regulated reporter gene expression in the moss Physcomytrella patens. Plant Mol Biol 1996, in press.
19. Wirt E, Clayton C: Inducible gene expression in trypanosomes mediated by B prokaryotic repressor. Science 1995, 268:1179-1183.
20. Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, Bujard H: Transcriptional activation by tetracycline in mammalian cells. Science 1995, 268:1766-1769.
21. Klessig DF, Malamy J: The salicylic acid signal in plants. Plant Mol Biol 1994, 126:1439-1458.
22. Van da Rhee MD, Van Kan JAL, González-Jaén MT, Bol JF: Analysis of regulatory elements involved in the induction of two tobacco genes by salicylate treatment and virus infection. Plant Cell 1990, 2:357-366.
23. Uknes S, Dincher S, Friedrich L, Negrotto D, Williams S, Thompson-Taylor T, Potter S, Ward E, Ryals J: Regulation of pathogenesis-related protein-1a gene expression in tobacco. Plant Cell 1993, 5:159-169.
24. Williams S, Friedrich L, Dincher S, Carozzi N, Kessmann H, Ward E, Ryals J: Chemical regulation of Bacillus thuringiensis δ-endotoxin expression in transgenic plants. Biotechnology 1992, 10:540-543.
25. Strittmatter G, Janssens J, Opsomer C, Botterman J: Inhibition of fungal disease development in plants by engineering controlled cell death. Biotechnology 1995, 13:1085-1089.
26. Ulmasov T, Ohmiya A, Hagen G, Guilfoyle T: The soybean GH2/4 gene that encodes a gluthatione S-transferase has a promoter that is activated by a wide range of chemical agents. Plant Physiol 1995, 108:919-927.
27. Jepson I, Lay VJ, Holt DC, Bright SWJ, Greenland AJ: Cloning and characterization of maize herbicide safener-induced cDNAs encoding subunits of gluthatione S-transferase isoforms I, II and IV. Plant Mol Biol 1994, 26:1855-1866.