Generation of dual resistance to 4-hydroperoxycyclophosphamide and methotrexate by retroviral transfer of the human aldehyde dehydrogenase class 1 gene and a mutated dihydrofolate reductase gene

Molecular Therapy

Volumn 3, Issue 1, 2001, Pages 88-96

  Takebe, Naoko ^a   Zhao, Shi Cheng ^a   Adhikari, Debasis ^a   Mineishi, Shin ^a   Sadelain, Michel ^a   Hilton, John ^b   Colvin, Michael ^c   Banerjee, Debabrata ^a   Bertino, Joseph R ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

^c DUKE CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 HYDROPEROXYCYCLOPHOSPHAMIDE; ALDEHYDE DEHYDROGENASE; CD34 ANTIGEN; COMPLEMENTARY DNA; CYCLOPHOSPHAMIDE; DIHYDROFOLATE REDUCTASE; METHOTREXATE; PHENYLALANINE; RECOMBINANT GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; RECOMBINANT INTERLEUKIN 6; RECOMBINANT STEM CELL FACTOR; RECOMBINANT THROMBOPOIETIN; SERINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTINEOPLASTIC ACTIVITY; ARTICLE; BONE MARROW SUPPRESSION; BONE MARROW TRANSPLANTATION; CHEMOTHERAPY; COLONY FORMING UNIT GM; CONTROLLED STUDY; CROSS RESISTANCE; CYTOSOL; DEATH; DRUG MEGADOSE; DRUG PULSE THERAPY; DRUG RESISTANCE; DRUG TOLERABILITY; GENE CONSTRUCT; GENE MUTATION; GENE TRANSFER; GENETIC TRANSDUCTION; HEMATOPOIETIC CELL; HEMATOPOIETIC STEM CELL; IN VITRO STUDY; IN VIVO STUDY; IRRADIATION; LETHAL DOSE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; RADIATION DOSE; RETROVIRUS; TOXICITY; VIRUS INFECTION;

ANIMALIA; UNIDENTIFIED RETROVIRUS;

EID: 0034985885     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1006/mthe.2000.0236     Document Type: Article

References (37)

1. Selection of drug-resistant bone marrow cells in vivo after retroviral transfer of human MDR1
2. Use of safety modified retroviruses to introduce chemotherapy resistance sequences into normal hematopoietic cells for chemoprotection during the therapy of breast cancer: A pilot trial
3. Retroviral mediated transfer of the human multidrug resistance gene(MDR-1) into hematopoietic stem cells during autologous transplantation after intensive chemotherapy for metastatic breast cancer
4. A phase I clinical trial of retroviral-mediated transfer of the human MDR1 gene as marrow chemoprotection in patients undergoing high-dose chemotherapy and autologous stem-cell transplantation
5. Chemotherapy immediately following autologous stem-cell transplantation in patients with advanced breast cancer
6. Resistance to cytosine arabinoside by retrovirally mediated gene transfer of human cytidine deaminase into murine fibroblast and hematopoietic cells
7. Long-term protection of hematopoiesis against the cytotoxic effects of multiple doses of nitrosourea by retrovirus-mediated expression of human O6-alkylguanine DNA-alkyltransferase
8. Dose-intensive therapy in breast cancer
9. Ifosfamide, carboplatin, and etoposide: A highly effective cytoreduction and peripheral-blood progenitor-cell mobilization regimen for transplant-eligible patients with non-Hodgkin's lymphoma
10. Transduction of murine bone marrow cells with an MDR1 vector enables ex vivo stem cell expansion, but these expanded grafts cause a myeloproliferative syndrome in transplanted mice
11. Serial transplantation of methotrexate resistant bone marrow: Protection of murine recipients from drug toxicity by progeny of transduced stem cells
12. Development of a retroviral construct containing a human mutated dihydrofolate reductase cDNA for hematopoietic stem cell transduction
13. Protection of mice from lethal doses of methotrexate by transplantation with transgenic marrow expressing drug-resistant dihydrofolate reductase activity
14. Active site-directed double mutants of dihydrofolate reductase
15. Sensitization of hematopoietic stem and progenitor cells to trimetrexate using nucleoside transport inhibitors
16. + peripheral blood progenitor cells after introduction of a mutated dihydrofolate reductase cDNA via retroviral gene transfer
17. Role of aldehyde dehydrogenase in cyclophosphamide-resistant L1210 leukemia
18. Induction of cyclophosphamide-resistance by aldehyde-dehydrogenase gene transfer
19. Direct demonstration of elevated aldehyde-dehydrogenase in human hematopoietic progenitor cells
20. Construction and use of a safe and efficient amphotropic packaging cell line
21. Cloning and expression of the full-length cDNAs encoding human liver class 1 and class 2 aldehyde dehydrogenase
22. Methods for the construction of retroviral vectors and the generation of high-titer producers
23. Retroviral-mediated transfer of the green fluorescent protein gene into murine hematopoietic cells facilitates scoring and selection of transduced progenitors in vitro and identification of genetically modified cells in vivo
24. Co-expression of the herpes simplex virus thymidine kinase gene potentiates methotrexate resistance conferred by transfer of a mutated dihydrofolate reductase gene
25. An improved colorimetric assay for cell protection and viability utilizing the tetrazolium salt XTT
26. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction
27. 36B4 cDNA used as an estradiol-independent mRNA control is the cDNA for human acidic ribosomal phosphoprotein PO
28. Method for detection of specific RNAs in agarose gels by transfer to diazobenzoyloxymethyl-paper and hybridization with DNA probes
29. Role of E2F-1 in chemosensitivity
30. Identification of the mouse aldehyde dehydrogenases important in aldophosphamide detoxification
31. Increased gene transfer into human hematopoietic progenitor cells by extended in vitro exposure to a pseudotyped retroviral vector
32. Post-transplant methotrexate administration leads to improved curability of mice bearing a mammary tumor transplanted with marrow transduced with a mutant human dihydrofolate reductase cDNA
33. + cells from a Gaucher patient
34. Coding region-specific destabilization of mRNA transcripts attenuates expression from retroviral vectors containing class I aldehyde dehydrogenase cDNAs
35. In vitro selection for K562 cells with higher retrovirally mediated copy number of aldehyde dehydrogenase class-I and higher resistance to 4-hydroperoxycyclophosphamide
36. Long term protection of recipient mice from lethal doses of methotrexate by marrow infected with a double copy vector retrovirus containing a mutant dihydrofolate reductase
37. In vivo selection of retrovirally transduced hematopoietic stem cells