A proteolytic method for distinguishing between lipid-free and lipid-bound apolipoprotein A-I

Journal of Lipid Research

Volumn 42, Issue 5, 2001, Pages 864-872

  Safi, W ^a   Maiorano, J N ^a   Davidson, W S ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

Cholesterol; Enteropeptidase; Lipid free apoA I; Lipid poor apoA I; Lipoprotein; Pre HDL

Indexed keywords

AMINO ACID; APOLIPOPROTEIN A1; CHOLESTEROL; ENTEROPEPTIDASE; HIGH DENSITY LIPOPROTEIN; LIPID;

ARTICLE; CONFORMATIONAL TRANSITION; DENSITOMETRY; DISSOCIATION; HUMAN; IN VITRO STUDY; LIPID ANALYSIS; LIPOPROTEIN BLOOD LEVEL; MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION;

APOLIPOPROTEIN A-I; BIOLOGICAL ASSAY; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENTEROPEPTIDASE; HUMANS; LIPID METABOLISM; PROTEIN BINDING; PROTEIN CONFORMATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0034980877     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (47)

1. Comparison of apo A-I-containing subpopulations of dog plasma and prenodal peripheral lymph: Evidence for alteration in subpopulations in the interstitial space
2. Presence and formation of 'free apolipoprotein A-I-like' particles in human plasma
3. Characterization of dog prenodal peripheral lymph lipoproteins. Evidence for the peripheral formation of lipoprotein-unassociated apoA-I with slow pre-beta electrophoretic mobility
4. Apolipoprotein A-I-containing lipoproteins with pre-beta electrophoretic mobility
5. Unique epitope of apolipoprotein A-I expressed in pre-beta-1 high-density lipoprotein and its role in the catalyzed efflux of cellular cholesterol
6. Early incorporation of cell-derived cholesterol into pre-beta-migrating high-density lipoprotein
7. Role of free apolipoprotein A-I in cholesterol efflux. Formation of pre-alpha-migrating high-density lipoprotein particles
8. Pre-beta high-density lipoprotein determined by immunoblotting with chemiluminescent detection
9. Prebeta-1 HDL in plasma of normolipidemic individuals: Influences of plasma lipoproteins, age, and gender
10. ABCA1-mediated transport of cellular cholesterol and phospholipids to HDL apolipoproteins
11. Tangier disease is caused by mutations in the gene encoding ATP-binding cassette transporter 1
12. The gene encoding ATP-binding cassette transporter 1 is mutated in Tangier disease
13. Mutations in ABC1 in Tangier disease and familial high-density lipoprotein deficiency
14. Altered properties of high density lipoprotein subfractions in obese subjects
15. Biochemical characterization of pre-beta 1 high-density lipoprotein from human ovarian follicular fluid: Evidence for the presence of a lipid core
16. Very small apolipoprotein A-I-containing particles from human plasma: Isolation and quantification by, high-performance size-exclusion chromatography
17. Preparative free-solution isotachophoresis for separation of human plasma lipoproteins: Apolipoprotein and lipid composition of HDL subfractions
18. Analytical capillary isotachophoresis of human serum lipoproteins
19. Packing of cholesterol molecules in human low-density lipoprotein
20. The orientation of helix 4 in apolipoprotein A-I-containing reconstituted high density lipoproteins
21. Sterol to phospholipid molar ratios of L cells with qualitative and quantitative variations of cellular sterol
22. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples
23. Effects of acceptor particle size on the efflux of cellular free cholesterol
24. Effect of apolipoprotein A-I lipidation on the formation and function of pre-beta and alpha-migrating LpA-I particles
25. High yield overexpression and characterization of human recombinant proapolipoprotein A-I
26. Structural characterization of porcine enteropeptidase
27. Autolysis of bovine enteropeptidase heavy chain: Evidence of fragment 118-465 involvement in trypsinogen activation
28. Specificity of bovine enterokinase toward protein substrates
29. Structural analysis of apolipoprotein A-I: Effects of amino- and carboxy-terminal deletions on the lipid-free structure
30. Structural models of human apolipoprotein A-I
31. On the mechanism of the displacement of apolipoprotein A-I by apolipoprotein A-II from the high density lipoprotein surface. Effect of concentration and molecular forms of apolipoprotein A-II
32. Displacement of apo A-I from HDL by apo A-II or its C-terminal helix promotes the formation of pre-beta1 migrating particles and decreases LCAT activation
33. The effect of apolipoprotein A-II on the structure and function of apolipoprotein A-I in a homogeneous reconstituted high density lipoprotein particle
34. Interconversion of pre-beta-migrating lipoproteins containing apolipoprotein A-I and HDL
35. The molecular basis for the difference in charge between pre-beta- and alpha-migrating high density lipoproteins
36. Differential effects of lecithin and cholesterol on the immunoreactivity and conformation of apolipoprotein A-I in high density lipoproteins
37. The epitopes of apolipoprotein A-I define distinct structural domains including a mobile middle region
38. Localization of two epitopes of apolipoprotein A-I that are exposed on human high density lipoproteins using monoclonal antibodies and synthetic peptides
39. Enzymatic hydrolysis of reconstituted dimyristoylphosphatidylchholine-apo A-I complexes
40. Pre-beta high density lipoprotein. Unique disposition of apolipoprotein A-I increases susceptibility to proteolysis
41. Structural and functional domains of apolipoprotein A-I within high density lipoproteins
42. Properties of an N-terminal proteolytic fragment of apolipoprotein AI in solution and in reconstituted high density lipoproteins
43. Structural analysis of apolipoprotein A-I limited proteolysis of methionine-reduced and -oxidized lipid-free and lipid-bound human apo A-I
44. Interconversion between apolipoprotein A-I-containing lipoproteins of pre-beta and alpha electrophoretic mobilities
45. Cycling of apolipoprotein A-I between lipid-associated and lipid- free pools
46. Human apolipoprotein A-II inhibits the formation of pre-beta high density lipoproteins
47. Structure of human serum lipoproteins inferred from compositional analysis