Expression and purification of monospecific and bispecific recombinant antibody fragments derived from antibodies that block the CD80/CD86-CD28 costimulatory pathway

Protein Expression and Purification

Volumn 22, Issue 1, 2001, Pages 11-24

  Dincq, Stéphanie ^a   Bosman, Fons ^a   Buyse, Marie Ange ^a   Degrieck, Rosina ^a   Celis, Linda ^a   Boer, Marc De ^b   Van Doorsselaere, Veronique ^a   Sablon, Erwin ^a  

^a INNOGENETICS   (Belgium)

^b Tanox Pharma BV   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

B7 ANTIGEN; BACTERIAL PROTEIN; CD28 ANTIGEN; CD86 ANTIGEN; COMPLEMENTARY DNA; ENZYME LINKED IMMUNOSORBENT ASSAY; HYBRID PROTEIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; SPECIFICITY;

ESCHERICHIA COLI;

EID: 0034978577     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1417     Document Type: Article

References (29)

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6. Transplantation tolerance induced by CTLA-4Ig
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11. A dimeric bispecific miniantibody combines two specificities with avidity
12. Functional characterization of a novel anti B7 monoclonal antibody
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14. Protein thiolation and reversible protein-protein conjugation: N-succinimidyl-3-(2-pyridyldithio) propionate, a new heterobifunctional reagent
15. Both LFA-1-positive and deficient T cell clones require the CD2/LFA-3 interaction for specific cytolytic activation
16. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
17. Single step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides
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19. A new family of sugar inducible expression vectors for E. coli
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21. Regulatory sequences involved in the promotion and termination of RNA transcriptie
22. Overexpression of mRNA dependent on rare codons inhibits protein synthesis and cell growth
23. Construction, expression, and activity of a bivalent bispecific single-chain antibody
24. Solubility and secretability
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29. One hundred seventy fold increase in excretion of a Fv fragment-tumor necrosis factor alfa fusion protein from E. coli caused by synergistic effects of glycine and Triton X-100