Aberrant mobility phenomena of the DNA repair protein XPA

Protein Science

Volumn 10, Issue 7, 2001, Pages 1353-1362

  Iakoucheva, Lilia M ^a,c   Kimzey, Amy L ^a   Masselon, Christophe D ^b   Smith, Richard D ^b   Dunker, A Keith ^c   Ackerman, Eric J ^a  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^c WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

DNA repair; ESI FTICR mass spectrometry; Gel electrophoresis; Intrinsic disorder; Partial proteolysis; SDS PAGE; XPA

Indexed keywords

CISPLATIN; CYSTEINE; DNA; GLUCOSE; GLUTARALDEHYDE; MONOMER; PROTEIN; PROTEIN XPA; TRYPSIN; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; CYCLOTRON; DNA BINDING; DNA REPAIR; EXCISION REPAIR; FOURIER TRANSFORMATION; GEL FILTRATION CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN MODIFICATION; PROTEIN PURIFICATION; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN TRANSPORT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TRANSLATION REGULATION; XENOPUS;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, GEL; CISPLATIN; CROSS-LINKING REAGENTS; DNA-BINDING PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OLIGONUCLEOTIDES; PEPTIDE FRAGMENTS; RNA-BINDING PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SULFHYDRYL COMPOUNDS; XENOPUS; XERODERMA PIGMENTOSUM GROUP A PROTEIN;

EID: 0034967777     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.40101     Document Type: Article

References (46)

1. Nucleotide excision repair in oocyte nuclear extracts from Xenopus laevis
2. The anomalous electrophoretic behavior of the human papillomavirus type 16 E7 protein is due to the high content of acidic amino acid residues
3. The XPA protein is a zinc metalloprotein with an ability to recognize various kinds of DNA damage
4. Anomalous electrophoretic mobility of Drosophila phosphorylated H1 histone: Is it related to the compaction of satellite DNA into heterochromatin?
5. Probing the reactivity of S-S bridges to acrylamide in some proteins under high pH conditions by matrix-assisted laser desorption/ionisation
6. Structural features of the minimal DNA binding domain (M98-F219) of human nucleotide excision repair protein XPA
7. Extended X-ray absorption fine structure evidence for a single metal binding domain in Xenopus laevis nucleotide excision repair protein XPA
8. Recognition of nonhybridizing base pairs during nucleotide excision repair of DNA
9. Guide to protein purification
10. Mobility of sodium dodecyl sulphate-protein complexes
11. Observations on molecular weight determinations on polyacrylamide gel
12. Probing the conformational state of apomyoglobin by limited proteolysis
13. Bioconjugate Techniques
14. Bipartite substrate discrimination by human nucleotide excision repair
15. Counting integral numbers of residues by chemical modification
16. The conserved, hydrophilic and arginine-rich N-terminal domain of cucumovirus coat proteins contributes to their anomalous electrophoretic mobilities in sodium dodecylsulfate-polyacrylamide gels
17. Identification of intrinsic order and disorder in the DNA repair protein XPA
18. Solution structure of the DNA- and RPA-binding domain of the human repair factor XPA
19. Preferential binding of the xeroderma pigmentosum group A complementing protein to damaged DNA
20. Molecular weight abnormalities of the CTCF transcription factor: CTCF migrates aberrantly in SDS-PAGE and the size of the expressed protein is affected by the UTRs and sequences within the coding region of the CTCF gene
21. Structural studies of p21Waf1/Cip1/Sdi1 in the free and Cdk2-bound state: Conformational disorder mediates binding diversity
22. Probing protein structure using biochemical and biophysical methods. Proteolysis, matrix-assisted laser desorption/ionization mass spectrometry, high-performance liquid chromatography and size-exclusion chromatography of p21Waf1/Cip1/Sdi1
23. Identification of a damaged-DNA binding domain of the XPA protein
24. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
25. Replication protein A interactions with DNA. III. Molecular basis of recognition of damaged DNA
26. Quality control by DNA repair
27. Statistical determination of the average values of the extinction coefficients of tryptophan and tyrosine in native proteins
28. Anomalous behavior of the major avian myeloblastosis virus glycoprotein in the presence of sodium dodecyl sulfate
29. Mutational analysis of the structure and function of the xeroderma pigmentosum group A complementing protein. Identification of essential domains for nuclear localization and DNA excision repair
30. Differential human nucleotide excision repair of paired and mispaired cisplatin-DNA adducts
31. Isolation of proteins for microsequence analysis
32. Recognition and repair of compound DNA lesions (base damage and mismatch) by human mismatch repair and excision repair systems
33. Probing conformations of amyloidogenic proteins by hydrogen exchange and mass spectrometry
34. DNA polymerases a and b are required for DNA repair in an efficient nuclear extract from Xenopus oocytes
35. The binding of sodium dodecyl sulphate to various proteins
36. Molecular weight estimation using sodium dodecyl sulfate - Pore gradient electrophoresis
37. A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein
38. Probing the native structure of stathmin and its interaction domains with tubulin. Combined use of limited proteolysis, size exclusion chromatography, and mass spectrometry
39. The gross conformation of protein-sodium dodecyl sulfate complexes
40. Evidence for sodium dodecyl sulfate/protein complexes adopting a necklace structure
41. Free-boundary electrophoresis of sodium dodecyl sulfate-protein polypeptide complexes with special reference to SDS-polyacrylamide gel electrophoresis
42. Distribution of mutations in the human xeroderma pigmentosum group A gene and their relationships to the functional regions of the DNA damage recognition protein
43. Reevaluation of the electrophoretic migration behavior of soluble globular proteins in the native and detergent-denatured states in polyacrylamide gels
44. Intrinsically unstructured proteins: Re-assessing the protein structure-function paradigm
45. Electrospray ionization-mass spectrometry study of the interaction of cisplatin-adducted oligonucleotides with human XPA minimal binding domain protein