Development of a six-stage culture system for simulating the gastrointestinal microbiota of weaned infants

Microbial Ecology in Health and Disease

Volumn 13, Issue 2, 2001, Pages 111-123

  De Boever, P ^a   Wouters, R ^a   Vermeirssen, V ^a   Boon, N ^a   Verstraete, W ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Culture system; Gut fermentation; Infant; Intestinal microbiota

Indexed keywords

AMMONIA; FATTY ACID; HEXANOIC ACID; PROPIONIC ACID; SUGAR; VALERIC ACID;

ARTICLE; ARTIFICIAL MILK; CHILD; CONTROLLED STUDY; CULTURE MEDIUM; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA FINGERPRINTING; FECES ANALYSIS; FERMENTATION; GASTROINTESTINAL ABSORPTION; HUMAN; INFANT FEEDING; INTESTINE FLORA; NORMAL HUMAN; PRIORITY JOURNAL; WEANING;

MEGASPHAERA; MICROBIOTA;

EID: 0034963890     PISSN: 0891060X     EISSN: None     Source Type: Journal    
DOI: 10.1080/089106001300136183     Document Type: Article

References (59)

1. Creating and maintaining the gastrointestinal ecosystem: What we know and need to know from gnotobiology
2. Intestinal flora of infants
3. Microbial ecology of the large bowel of breast-fed and formula-fed infants during the first year of life
4. Diet and faecal flora in the newborn: Iron
5. The effect of humanised milks and supplemented breast feeding on the faecal flora of infants
6. Development of intestinal microbiota
7. Normal alimentary tract microflora
8. Overview of gut flora and probiotics
9. Colonic sulfide in pathogenesis and treatment of ulcerative colitis
10. Effects of calcium and deoxycholic acid on human colonic cell proliferation in vitro
11. Human colonic microbiota: Ecology, physiology and metabolic potential of intestinal bacteria
12. In vivo and in vitro models of the human colonic flora
13. Polysaccharide degradation by human intestinal bacteria during growth under multi-substrate limiting conditions in a three-stage continuous culture system
14. Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon
15. Influence of retention time on degradation of pancreatic enzymes by human colonic bacteria grown in a 3-stage continuous culture system
16. Influence of mucin on glycosidase, protease and arylamidase activities of human gut bacteria grown in a 3-stage continuous culture system
17. A computer-controlled system to simulate conditions of the large intestine with peristaltic mixing, water absorption and absorption of fermentation products
18. In vitro fermentation of carbohydrate by breast fed and formula fed infants
19. Characterisation of carbohydrate fermentation in faeces of formula fed and breast fed infants
20. Faecal concentrations and production rates of short chain fatty acids in normal neonates
21. Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization
22. Statistical comparisons of community catabolic profiles
23. Monitoring Biolog patterns and r/K strategists in the intensive culture of Artemiai juveniles
24. Evaluation of community-level catabolic profiling using BIOLOG GN microplates to study microbial community changes in potato phyllosphere
25. Comparison of the spatial homogeneity of physico-chemical parameters and bacterial 16S rRNA genes in sediment samples of a dumping site of dredging sludge
26. Effect of phenylurea herbicides on soil microbial communities estimated by analysis of 16S rRNA gene fingerprints and community-level physiological profiles
27. Distribution of bacterioplankton in meromictic lake Saelevannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA
28. Pofiling of complex microbial populations using denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
29. Denaturing gradient gel electrophoresis of PCR amplified 16S rDNA-a new molecular approach to analyse the genetic diversity of mixed microbial communities
30. Faecal tryptic activity in humans: Influence of antibiotics on microbial intestinal degradation
31. Effect of 2-bromoethane-sulfonic acid and Peptostreptoccus productus ATCC 35244 addition on stimuation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis
32. Steam distillation methods for determination of ammonium, nitrate and glycine
33. Validation of the simulator of the human intestinal microbial ecosystem (SHIME) reactor using microorganism-associated activities
34. Development of a 5-step multi-chamber reactor as a simulation of the human intestinal microbial ecosystem
35. Comparison of fermentation reactions in different regions of the human colon
36. Pattern of metabolism and composition of the faecal microflora in infants 10 to 18 months old from day care centers
37. The bowel habit of young children
38. Bowel behaviour in young black and white children
39. Randomised controlled trial of trophic feeding and gut motility
40. Use of a three-stage continuous culture system to study the effect of mucin on dissimilatory sulfate reduction and methanogenesis by mixed populations of human gut bacteria
41. The control and consequences of bacterial fermentation in the human colon
42. The metabolic importance of unabsorbed dietary lipids in the colon
43. Does digestibility of meat protein help explain large bowel cancer risk
44. Developmental microbial ecology of the neonatal gastrointestinal tract
45. Relationship between oxygen sensitivity and oxygen metabolism of Bifidobacterium sp.
46. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria
47. Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers
48. Analysis of BIOLOG GN substrate utilization patterns by microbial communities
49. Application of denaturing gradient gel electrophoresis for the analysis of the porcine gastrointestinal microbiota
50. The gut microflora of the developing infant: Microbiology and metabolism
51. Production of short chain fatty acids by the intestinal microflora during the first 2 years of human life
52. Pathways of acetate, propionate, and butyrate formation by the human fecal microbial flora
53. Concentrations and correlations of faecal short-chain fatty acids and faecal water content in man
54. Gastro-enteric methane versus sulphate and volatile fatty acid production
55. Alternative hydrogen sink pathways in hindgut fermentation
56. Metabolism of lactate and sugars by dairy propionibacteria: A review
57. Section 8 Anaerobic Gram negative cocci
58. Substrate preference in a strain of Megaspaera elsdenii, a ruminal bacterium, and its implication in propionate production and growth competition
59. Isolation of Clostridium propionicum strain 19aery3 and further characteristics of the species