Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon

Microbial Ecology

Volumn 35, Issue 2, 1998, Pages 180-187

  Macfarlane, G T ^b   Macfarlane, S ^b   Gibson, G R ^a,b  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^b MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID METABOLISM; CARBOHYDRATE METABOLISM; CLOSTRIDIUM PERFRINGENS; CULTURE MEDIUM; ESCHERICHIA COLI; FATTY ACID METABOLISM; MICROBIAL FUNCTION;

CLOSTRIDIUM PERFRINGENS;

EID: 0031944894     PISSN: 00953628     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002489900072     Document Type: Article

References (21)

1. Allison C, McFarlan C, Macfarlane GT (1989) Studies on mixed populations of human intestinal bacteria grown in single and multistage continuous culture systems. Appl Environ Microbiol 55:679-683
2. Cowan ST (1974) Cowan and Steel's manual for the identification of medical bacteria. Cambridge University Press, Cambridge
3. Cummings JH, Macfarlane GT (1991) The control and consequences of bacterial fermentation in the human colon - a review. J Appl Bacteriol 70:443-459
4. Cummings JH, Hill MJ, Bone ES, Branch WJ, Jenkins DJA (1979) The effect of meat protein and dietary fiber on colonic function and metabolism. Part II. Bacterial metabolites in feces and urine. Am J Clin Nutr 32:2094-2101
5. Cummings JH, Pomare EW, Branch WJ, Naylor CPE, Macfarlane GT (1987) Short chain fatty acids in human large intestine, portal, hepatic, and venous blood. Gut 28:1221-1227
6. Cummings JH, Bingham SA, Heaton KW, Eastwood MA (1993) Fecal weight, colon cancer risk, and dietary intake of non-starch polysaccharides (dietary fiber). Gastroenterology 103:1783-1789
7. Degnan BA, Macfarlane GT (1995) Arabinogalactan utilization in continuous cultures of Bifidobacterium longum: Effect of co-culture with Bacteroides thetaiotaomicron. Anaerobe 1: 103-112
8. Englyst HN, Quigley ME, Hudson GJ (1994) Determination of dietary fibre as non-starch polysaccharides with gas-liquid chromatographic, high performance liquid chromatographic, or spectrophotometric measurement of constituent sugars. Analyst 119:1479-1509
9. Finegold SM, Sutter VL, Mathisen GE (1983) Normal indigenous intestinal flora. In: Hentges DJ (ed) Human intestinal microflora in health and disease. Academic Press, New York, pp 3-31
10. Holdeman LV, Cato EP, Moore WEC (eds) (1977) Anaerobic laboratory manual, 4th edn. Virginia Polytechnic Institute Anaerobe Laboratory, Blacksburg
11. Macfarlane GT, Cummings JH (1991) The colonic flora, fermentation, and large bowel digestive function. In: Phillips SF, Pemberton JH, Shorter RG (eds) The large intestine: physiology, pathophysiology, and disease. Raven Press, New York, pp 51-92
12. Macfarlane GT, Englyst HN (1986) Starch utilization by the human large intestinal microflora. J Appl Bacteriol 60:195-201
13. Macfarlane GT, Gibson GR (1994) Metabolic activities of the normal colonic flora. In: Gibson SAW (ed) Human health: the contribution of microorganisms. Springer-Verlag, London, pp 17-52
14. Macfarlane GT, Gibson GR (1995) Microbiological aspects of short chain fatty acid production in the large bowel. In: Cummings JH, Rombeau JL, Sakata T (eds) Physiological and clinical aspects of short chain fatty acid metabolism. Cambridge University Press, Cambridge, pp 87-105
15. Macfarlane S, Macfarlane GT (1995) Proteolysis and amino acid fermentation. In: Gibson GR, Macfarlane GT (eds) Human colonic bacteria: role in nutrition, physiology, and pathology. CRC Press, Boca Raton, pp 75-100
16. Macfarlane GT, Hay S, Macfarlane S, Gibson GR (1990) Effect of different carbohydrates on growth, polysaccharidase and glycosidase production by Bacteroides ovatus, in batch and continuous culture. J Appl Bacteriol 68:179-187
17. Macfarlane GT, Gibson GR, Cummings JH (1992) Comparison of fermentation reactions in different regions of the human colon. J Appl Bacteriol 72:57-64
18. Macfarlane GT, Gibson GR, Macfarlane S (1994) Short chain fatty acid and lactate production by human intestinal bacteria grown in batch and continuous culture. In: Binder HJ, Cummings JH, Soergel KH (eds) Short chain fatty acids. Kluwer Publishing, London, pp 44-60
19. Macfarlane GT, Gibson GR, Drasar BS, Cummings JH (1995) Metabolic significance of the colonic microflora. In: Whitehead R (ed) Gastrointestinal and oesophageal physiology. Churchill Livingstone, Edinburgh, pp 249-274
20. Quigley ME, Englyst HN (1992) Determination of neutral sugars and hexosamines by high-performance liquid chromatography with pulsed amperometric detection. Analyst 117: 1715-1718
21. Wiggins HS, Cummings JH (1976) Evidence for the mixing of residue in the human gut. Gut 17:1007-1011