Purification of a bacterially expressed herpes simplex virus type 1 origin binding protein for use in posttranslational processing studies

Protein Expression and Purification

Volumn 22, Issue 2, 2001, Pages 276-285

  Bronstein, Joel C ^a   Weber, Peter C ^a  

^a PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMATOGRAPHY; CYCLIC AMP DEPENDENT PROTEIN KINASE; DNA SYNTHESIS; HERPES SIMPLEX VIRUS; OPEN READING FRAME; PHOSPHORUS 32; PROTEIN BINDING; PROTEIN MODIFICATION; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; VIRUS DNA; VIRUS INFECTION; VIRUS INFECTIVITY; VIRUS PROTEIN; VIRUS REPLICATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HERPES; HUMAN HERPESVIRUS 1; MAMMALIA;

EID: 0034962757     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1446     Document Type: Article

References (59)

1. Replication of herpes simplex virus DNA
2. Herpes simplex virus DNA replication
3. Herpes simplex virus DNA replication: The UL9 gene encodes an origin-binding protein
4. Interaction of origin binding protein with an origin of replication of herpes simplex virus 1
5. Two binding sites for the herpes simplex virus type 1 UL9 protein are required for efficient activity of the oriS replication origin
6. The herpes simplex virus 1 origin binding protein: A DNA helicase
7. Purification and characterization of UL9, the herpes simplex virus type 1 origin-binding protein
8. The herpes simplex virus type I origin binding protein. DNA-dependent nucleoside triphosphatase activity
9. The herpes simplex virus type-1 origin binding protein. DNA helicase activity
10. Characterisation of the nucleotide and DNA coeffector binding sites of the herpes simplex virus type 1 (HSV-1) encoded helicase-primase complex and UL9 origin binding protein
11. The herpes simplex virus type 1 origin-binding protein. Sequence-specific activation of adenosine triphosphatase activity by a double-stranded DNA containing box I
12. The herpes simplex virus type-1 single-strand DNA-binding protein, ICP8, increases the processivity of the UL9 protein DNA helicase
13. Interaction between the herpes simplex virus type 1 origin-binding and DNA polymerase accessory proteins
14. The herpes simplex virus type 1 origin-binding protein interacts specifically with the vital UL8 protein
15. Interaction of herpes simplex virus 1 origin-binding protein with DNA polymerase alpha
16. Herpes simplex virus phosphoproteins. II. Characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion
17. Herpes simplex virus phosphoproteins. I. Phosphate cycles on and off some viral polypeptides and can alter their affinity for DNA
18. Domain separation in the activation of glycogen phosphorylase a
19. Structural changes in glycogen phosphorylase induced by phosphorylation
20. The allosteric transition of glycogen phosphorylase
21. Regulation of an enzyme by phosphorylation at the active site
22. Identification of phosphorylation sites within the herpes simplex virus tegument protein VP22
23. Phosphorylation of structural components promotes dissociation of the herpes simplex virus type 1 tegument
24. Identification and characterization of the virion-induced host shutoff product of herpes simplex virus gene UL41
25. Characterization of the novel protein kinase activity present in the R1 subunit of herpes simplex virus ribonucleotide reductase
26. Studies on the herpes simplex virus alkaline nuclease: Detection of type-common and type-specific epitopes on the enzyme
27. Characterization of herpes simplex virus 1 alpha proteins 0, 4, and 27 with monoclonal antibodies
28. Tyrosine phosphorylation of the herpes simplex virus type 1 regulatory protein ICP22 anda cellular protein which shares antigenic determinants with ICP22
29. Analysis of phosphorylation sites of herpes simplex virus type 1 ICP4
30. Analysis of the phosphorylation sites of herpes simplex virus type 1 regulatory protein ICP27
31. The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1
32. Phosphorylation of the herpes simplex virus type 1 origin binding protein
33. Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase alpha-primase
34. SV40 T antigen binds directly to the large subunit of purified DNA polymerase alpha
35. Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen
36. In vitro phosphorylation of SV40 large T antigen
37. Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication
38. Structure and function of simian virus 40 large tumor antigen
39. Regulation of SV40 DNA replication by phosphorylation of T antigen
40. The replication functions of SV40 T antigen are regulated by phosphorylation
41. A subset of herpes simplex virus replication genes induces DNA amplification within the host cell genome
42. A 269-amino-acid segment with a pseudo-leucine zipper and a helix-turn-helix motif codes for the sequence-specific DNA-binding domain of herpes simplex virus type 1 origin-binding protein
43. Purification and characterization of herpes simplex virus type 1 alkaline exonuclease expressed in Escherichia coli
44. An improved assay for nanomole amounts of inorganic phosphate
45. A DNA helicase induced by herpes simplex virus type 1
46. A DNA binding protein specific for an origin of replication of herpes simplex virus type 1
47. Herpes simplex virus type 1 gene products required for DNA replication: Identification and overexpression
48. Nucleoprotein complex formed between herpes simplex virus UL9 protein and the origin of DNA replication: Inter- and intramolecular interactions
49. Binding of the herpes simplex virus type 1 UL9 gene product to an origin of viral DNA replication
50. Structural elements required for the cooperative binding of the herpes simplex virus origin binding protein to oriS reside in the N-terminal part of the protein
51. The origin binding protein of herpes simplex virus 1 binds cooperatively to the viral origin of replication oris
52. Activation of second-messenger pathways reactivates latent herpes simplex virus in neuronal cultures
53. Repression of viral transcription during herpes simplex virus latency
54. Tissue-specific accumulation of latency-associated transcripts in herpes virus-infected rabbits
55. Gene expression during reactivation of herpes simplex virus type 1 from latency in the peripheral nervous system is different from that during lytic infection of tissue cultures
56. Quantification of transcripts from the ICP4 and thymidine kinase genes in mouse ganglia latently infected with herpes simplex virus
57. Restricted expression of herpes simplex virus lytic genes during establishment of latent infection by thymidine kinase-negative mutant viruses
58. Herpes simplex virus gene expression in neurons: Viral DNA synthesis is a critical regulatory event in the branch point between the lytic and latent pathways
59. Cell-type-specific induction of the UL9 gene of HSV-1 by cell signaling pathway