Purification and characterization of hexahistidine-tagged cyclohexanone monooxygenase expressed in Saccharomyces cerevisiae and Escherichia coli

Protein Expression and Purification

Volumn 21, Issue 1, 2001, Pages 81-86

  Cheesman, Matthew J ^a   Kneller, M Byron ^a   Kelly, Edward J ^a   Thompson, Stella J ^a   Yeung, Catherine K ^a   Eaton, David L ^a   Rettie, Allan E ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; BACTERIAL ENZYME; CARBOXY TERMINAL SEQUENCE; CYCLOHEXANONE MONOOXYGENASE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; FLAVOPROTEIN; HETEROLOGOUS EXPRESSION; HISTIDINE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR WEIGHT; REACTION MECHANISM; SITE DIRECTED MUTAGENESIS;

ACINETOBACTER; ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 0034951924     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1340     Document Type: Article

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