Discrepancy between polymerase chain reaction assay and western blot analysis in the assessment of CagA status in dyspeptic patients

Helicobacter

Volumn 6, Issue 2, 2001, Pages 130-135

  Paoluzi, Omero Alessandro ^a,d   Rossi, Pina ^b   Montesano, Carla ^c   Bernardi, Sabrina ^a   Carnieri, Emanuela ^d   Marchione, Orazio Paolo ^b   Nardi, Francesco ^a   Iacopini, Federico ^c   Pica, Roberta ^a   Paoluzi, Paolo ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

^c Laboratory of Clinical Diagnosis PRODA   (Italy)

^d POLICLINICO UMBERTO I   (Italy)

Author keywords

CagA; Helicobacter pylori; Polymerase chain reaction

Indexed keywords

ADULT; ARTICLE; BACTERIAL GENE; CLINICAL ARTICLE; CONTROLLED STUDY; DUODENUM ULCER; DYSPEPSIA; FEMALE; GASTROINTESTINAL ENDOSCOPY; GRAM NEGATIVE INFECTION; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STOMACH BIOPSY; WESTERN BLOTTING;

ADULT; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BIOPSY; BLOTTING, WESTERN; DUODENUM; DYSPEPSIA; ENDOSCOPY, DIGESTIVE SYSTEM; FEMALE; GASTRIC MUCOSA; GENES, BACTERIAL; HELICOBACTER PYLORI; HUMANS; INTESTINAL MUCOSA; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; UREASE;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI; NEGIBACTERIA;

EID: 0034951025     PISSN: 10834389     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-5378.2001.00019.x     Document Type: Article

References (15)

1. Cumulative 10-years risk of symptomatic duodenal and gastric ulcer in patients with or without chronic gastritis: A clinical follow-up study
2. Cloning and expression of a high-molecular mass major antigen of Helicobacter pylori: Evidence of a linkage to cytotoxin production
3. Prevalence of CagA-bearing Helicobacter pylori strains detected by the anti-CagA assay in patients with peptic ulcer disease and in controls
4. Serologic detection of CagA positive Helicobacter pylori infection in a northern Italian population: Its association with peptic ulcer disease
5. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin
6. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer
7. Detection and identification of cagA of Helicobacter pylori by polymerase chain reaction
8. Serologic detection of infection with cagA+ Helicobacter pylori strains
9. Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains
10. The Sydney system: Histological division
11. Sensitive detection of Helicobacter pylori by using polymerase chain reaction
12. Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fingerprinting
13. Heterogeneous Helicobacter pylori isolates from members of a family with a history of peptic ulcer disease
14. CagA positive and negative Helicobacter pylori strains are simultaneously present in the stomach of most patients with non-ulcer dyspepsia: Relevance of histological damage
15. Validation of a serologic test for the diagnosis of Helicobacter pylori infection and the immune response to urease and CagA in children