One-step calibration of specimen induced focal shifts and spherical aberration for quantitative 3D microscopy: Approach and first results

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 4261, Issue , 2001, Pages 69-80

  Thomann, D ^a   Stussi E ^a   Danuser, G ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Calibration; Confocal Microscopy; Deconvolution; Focal Shifts; Spherical Aberration

Indexed keywords

ABERRATIONS; ALGORITHMS; BIOMEDICAL ENGINEERING; CALIBRATION; DIFFRACTION; IMAGE RECONSTRUCTION; INTERPOLATION; MEDICAL IMAGING; MULTIPHOTON PROCESSES; OPTICAL TRANSFER FUNCTION; REFRACTIVE INDEX; SIGNAL TO NOISE RATIO;

CONFOCAL MICROSCOPY; DECONVOLUTIONS; FOCAL SHIFTS;

OPTICAL MICROSCOPY;

EID: 0034945339     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.424521     Document Type: Conference Paper

References (11)

1. Aberrations in confocal fluorescence microscopy induced by mismatch in refractive index
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4. Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimens
5. Strategies for the compensation of specimen induced spherical aberration in confocal microscopy of skin
6. Parametric blind deconvolution: A robust method for simultaneous estimation of image and blur
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8. Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refraction indices: Structure of the elctromagnetic field
9. Equivalence of the huygens-fresnel and debye approach for the calculation of high aperture point-spread functions in the presence of refractive index mismatch
10. Model-based resolution: Applying the theory in quantitative microscopy
11. Photogrammetric calibration of a stereo light microscope