Crystal structure of methylmalonyl-coenzyme A epimerase from P. shermanii: A novel enzymatic function on an ancient metal binding scaffold

Structure

Volumn 9, Issue 7, 2001, Pages 637-646

  McCarthy, Andrew A ^a   Baker, Heather M ^a   Shewry, Steven C ^a   Patchett, Mark L ^c   Baker, Edward N ^a,b  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

^c MASSEY UNIVERSITY   (New Zealand)

Author keywords

Epimerization; Metal assisted catalysis; Metalloenzyme structure; Methylmalonyl CoA; Protein evolution; Vicinal oxygen chelate superfamily

Indexed keywords

2 METHYLMALONATE; BLEOMYCIN; BLEOMYCIN RESISTANCE PROTEIN; COBALT; ENZYME; EPIMERASE; EXTRADIOL DIOXYGENASE; GLUTAMIC ACID; GLYCINE; HISTONE; LACTOYLGLUTATHIONE LYASE; M PROTEIN; MALONIC ACID DERIVATIVE; METHYLMALONYL COENZYME A EPIMERASE; UNCLASSIFIED DRUG;

ARTICLE; BETA SHEET; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; GENE DUPLICATION; ISOMERISM; METAL BINDING; MOLECULAR EVOLUTION; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROPIONIBACTERIUM FREUDENREICHII SHERMANII; PROTEIN DOMAIN; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS;

BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; ENZYME STABILITY; EVOLUTION, MOLECULAR; METALS; MODELS, MOLECULAR; PROPIONIBACTERIUM; PROTEIN CONFORMATION; RACEMASES AND EPIMERASES;

ANIMALIA; BACTERIA (MICROORGANISMS); PROPIONIBACTERIUM; PROPIONIBACTERIUM FREUDENREICHII SUBSP. SHERMANII;

EID: 0034928937     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00622-0     Document Type: Article

References (45)

1. The metabolism of propionic acid
2. Disorders of propionate and methylmalonate metabolism
3. Stereospecific acy ltransfers on the erythromycin-producing polyketide synthase
4. Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli
5. Purification and characterization of methylmalonyl-CoA epimerase from Propionibacterium shermanii
6. Isolation and characterization of DL-methylmalonyl-Coenzyme A racemase from rat liver
7. Proton transfer in methylmalonyl-CoA epimerase from Propionibacterium shermanii. Studies with specifically tritiated (2R)-methylmalonyl-CoA as substrate
8. Mechanism of the reaction catalyzed by mandelate racemase. 1. Chemical and kinetic evidence for a two-base mechanism
9. RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase
10. The crystal structure of ADP-L-glycero-D-mannoheptose-6-epimerase: Catalysis with a twist
11. Understanding enzyme superfamilies: Chemistry as the fundamental determinant in the evolution of new catalytic activities
12. Elucidation of a monovalent cation dependence and characterization of the divalent cation binding site of the fosfomycin resistance protein (FosA)
13. Mechanistic diversity in a metalloenzyme superfamily
14. Crystal structure of human glyoxalase I - Evidence for gene duplication and 3D domain swapping
15. All in the family: Structural and evolutionary relationships among three modular proteins with diverse functions and variable assembly
16. Determination of macromolecular structures from anomalous diffraction of synchrotron radiation
17. Crystallography & NMR system: A new software suite for macromolecular structure determination
18. Phi/psi-cology: Ramachandran revisited
19. Protein-protein interactions: A review of protein dimer structures
20. Protein structure comparison by alignment of distance matrices
21. Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering
22. Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading Pseudomonad
23. Three-dimensional structures of free form and two substrate complexes of an extradiol ring-cleavage type dioxygenase, the BphC enzyme from Pseudomonas sp. strain KKS102
24. Crystal structure of Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase: An enzyme involved in the tyrosine degradation pathway
25. An archetypical extradiol-cleaving catecholic dioxygenase: The crystal structure of catechol 2,3-dioxygenase (metapyrocatechase) from Pseudomonas putida mt-2
26. Active monomeric and dimeric forms of Pseudomonas putida glyoxalase I: Evidence for 3D domain swapping
27. Determination of the structure of Escherichia coli glyoxalase I suggests a structural basis for differential metal activation
28. Reaction mechanism of glyoxalase I explored by an X-ray crystallographic analysis of the human enzyme in complex with a transition state analogue
29. Proton transfer in methylmalonyl-CoA epimerase from Propionibacterium shermanii. The reaction of (2R)-methylmalonyl-CoA in tritiated water
30. 28, models for metal complexes of γ-carboxyglutamic acid
31. Expression, crystallization and preliminary characterization of methylmalonyl coenzyme A epimerase from Propionibacterium shermanii
32. Processing of X-ray diffraction data collected in oscillation mode
33. The design and implementation of SnB version 2.0
34. Maximum likelihood refinement of heavy atom parameters
35. Miscellaneous algorithms for density modification
36. Improved methods for building protein models in electron density maps and the location of errors in these models
37. The free R value: A novel statistical quantity for assessing the accuracy of crystal structures
38. AMoRe: An automated package for molecular replacement
39. The CCP4 (Collaborative Computational Project Number 4) suite: Programs for protein crystallography
40. Hydrogen bonding in globular proteins
41. Molscript: A program to produce both detailed and schematic plots of protein structures
42. Raster3D: Photorealistic molecular graphics
43. Accurate bond and angle parameters for X-ray protein structure refinement